A microbiological screening method for the indication of irradiation of frozen poultry meat

Wirtanen, Gun; Salo, Satu; Karwoski, Merja; Sjöberg, Anna‐Maija

doi:10.1007/bf01190493

Cited by 7 publications

(4 citation statements)

References 9 publications

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“…DEFT is a method originally developed for the rapid enumeration of microorganisms in raw milk samples, 67 and it has been used for the detection on several foodstuffs, such as spices, beans, poultry, meat, and minimally processed vegetables. 66,[68][69][70][71][72][73][74][75] In this method, a specified volume of the sample is passed through a membrane filter to concentrate the microorganisms on the filter. The microorganisms are then stained with the fluorochrome acridine orange.…”

Section: Direct Epifluorescent Filter Technique Combined With Aerobicmentioning

confidence: 99%

“…As a result of the abovementioned studies, a log DEFT/APC ratio of 2.0 has been suggested as a threshold criterion for sample irradiation at doses of 0.5 kGy or higher. Nonetheless, this method has limitations when there are too few microbes in the sample (APCo10 3 CFU g À1 ) as the log DEFT/APC ratio can vary with the degree of initial contamination 69,76 and, for that reason, the suggested log DEFT/APC ratio should not be an absolute criterion. In addition, similar differences between DEFT and APC values can be induced by other food treatments leading to the death of microorganisms, such as heat, preservatives, or storage.…”

Section: Direct Epifluorescent Filter Technique Combined With Aerobicmentioning

confidence: 99%

“…The resulting higher loads of living microbes give rise to smaller differences between the DEFT and APC assessments and lower apparent levels of irradiation. 69 An advantage of the microbial method is that it provides additional information on the hygienic quality of the food. 77 The DEFT/APC method is specified in EN 13783:2001 as a screening method for the detection of irradiation treatment of herbs and spices, where a threshold criterion for irradiation of 3 to 4 is recommended.…”

Section: Direct Epifluorescent Filter Technique Combined With Aerobicmentioning

confidence: 99%

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Chapter 15. Biological Techniques

Rodrigues¹,

Venâncio²

2017

Food Chemistry, Function and Analysis

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DNA is a large molecule particularly sensitive to ionizing radiation, which suffers several kinds of damage: fragmentation resulting from both singlestrand and double-strand breaks, denaturation of the DNA helix, crosslinking (e.g., production of thymine dimers, or between DNA and a protein) and base damage. 1-3 It causes primarily single strand breaks (SSBs) in genomic DNA, in addition to double strand breaks (DSBs) at ratios of SSB/ DSB of 20/1 to 70/1, as well as some detectable membrane damage. 4 In foods, this DNA susceptibility is the cause of death of most if not all living contaminants, such as microorganisms, insects, or parasites, 4 and is also the cause of changes in the food's DNA itself, which can reflect on various morphological and physiological features. DNA damage occurs predominantly by the indirect action of gamma rays, which interact with other atoms or molecules, particularly water, to produce reactive free radicals. 5 Cell death (defined for proliferating cells as the loss of reproductive capability) is predominantly induced by double-strand breaks in DNA, separated by not more than a few base pairs, which cannot generally be repaired by the cell. 6 Since irradiation with just 1 Gy introduces about 1000 DNA single-strand breaks and about 50 double-strand breaks per cell, 7

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Section: Direct Epifluorescent Filter Technique Combined With Aerobicmentioning

confidence: 99%

Section: Direct Epifluorescent Filter Technique Combined With Aerobicmentioning

confidence: 99%

Section: Direct Epifluorescent Filter Technique Combined With Aerobicmentioning

confidence: 99%

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Chapter 15. Biological Techniques

Rodrigues¹,

Venâncio²

2017

Food Chemistry, Function and Analysis

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“…Outro trabalho, avaliando o método DEFT/APC a amostras de frango congeladas irradiadas com doses de 0; 3,0; 5,0 e 7,0kGy em acelerador de elétrons, encontraram valores médios da diferença entre log DEFT e log APC de 1,14; 3,16; 3,68 e 3,79 unidades logarítmicas, respectivamente. Neste estudo, propõem que uma diferença de pelo menos duas unidades logarítmicas podem ser consideradas o valor limite indicativo de um provável tratamento por radiação (Wirtanen et al, 1995).…”

Section: Método Deft/apcunclassified

Aplicação do método microbiológico DEFT/APC e do Teste do Cometa na detecção do tratamento com radiação ionizante de hortaliças minimamente processadas

Araújo¹

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O comércio de vegetais minimamente processados (VMP) tem crescido substancialmente nos últimos anos devido a sua conveniência, frescor e aparente salubridade. No entanto, o processamento mínimo não reduz as populações de microrganismos patogênicos para níveis seguros. A irradiação de alimentos é utilizada para estender a vida de prateleira e inativar patógenos presentes nos alimentos. Seu uso combinado com o processamento mínimo poderia aumentar a segurança e qualidade dos VMP. Dois diferentes métodos de detecção de alimentos irradiados, um biológico, o DEFT/APC, e outro bioquímico, o teste do cometa, foram aplicados a VMP com o objetivo de testar sua aplicabilidade na detecção do tratamento por radiação. O DEFT/APC é um método de varredura microbiológico baseado no uso da técnica de epifluorescência direta em filtro (DEFT) e da contagem padrão em placas (APC). O teste do cometa detecta o dano no DNA devido, por exemplo, a radiação ionizante. Amostras de acelga, agrião, alface, catalônia, couve, escarola, espinafre e repolho do comércio varejista foram irradiadas com 0,5kGy e 1,0kGy utilizando um irradiador de 60 Co. O processamento por irradiação garantiu a redução de pelo menos dois ciclos logarítmicos nas populações de microrganismos aeróbios e psicrotróficos. Em geral, com o aumento das doses de radiação, as contagens DEFT se mantiveram similares independentemente do processamento por irradiação, enquanto as contagens APC diminuíram gradualmente. A diferença das duas contagens aumentou gradualmente com o incremento da dose em todas as amostras. Uma diferença entre o valor de DEFT e do APC maior a 2,0 log seria indicativa de que o VMP foi tratado por irradiação. O teste do cometa permitiu distinguir amostras não irradiadas das irradiadas, que mostraram diferentes tipos de cometas decorrentes da fragmentação do DNA. Tanto o método DEFT/APC quanto o teste do cometa foram satisfatoriamente utilizados como métodos de varredura para a detecção do tratamento por irradiação.

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Nachweis der Behandlung von Lebensmitteln mit ionisierenden Strahlen

Marx

1998

Analytiker-Taschenbuch

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A microbiological screening method for the indication of irradiation of frozen poultry meat

Cited by 7 publications

References 9 publications

Chapter 15. Biological Techniques

Chapter 15. Biological Techniques

Aplicação do método microbiológico DEFT/APC e do Teste do Cometa na detecção do tratamento com radiação ionizante de hortaliças minimamente processadas

Nachweis der Behandlung von Lebensmitteln mit ionisierenden Strahlen

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