A microbiome record for life

Jia, Huijue

doi:10.1016/b978-0-323-91369-0.00005-4

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2024

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“…Metagenomic analyses of low-biomass environment such as tumors and placenta has been costly and controversial (10). Thus, a direct approach to characterize the microbes in tissues with over 99.9% host sequences would also be very much welcomed.…”

Section: Introductionmentioning

confidence: 99%

Single-cell omics for complex microbial communities

Lan,

Liang,

et al. 2024

Preprint

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Despite over a decade of effort, microbial single-cell genomics and transcriptomics remain a challenge, especially for complex communities such as the human microbiome. Here we report a solution based on the Laser-Induced Forward Transfer (LIFT) technology, which circumvent the need for droplet microfluidics and complex barcoding, and allow selective amplification of the genome and the transcriptome of commensal bacteria from complex samples. We capture the diverse oral microbiome, and elucidate the single-cell transcriptome in sporulation. This single-cell method is both scalable and precise, and enables investigation into tissue samples. The approach shows great promise for elucidating the single-cell fate of microbial populations and the native environment of the host-associated microbiome.

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Section: Introductionmentioning

confidence: 99%