A microcalorimetric method to study the activation of murine peritoneal macrophages

Gebreselassie, Daniel; Kasravi, Behzad; Bäckman, Per; Jeppson, Bengt

doi:10.1016/0040-6031(95)02723-8

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…Similar to other biological function, metabolism is the only source of energy required to perform phagocytosis (31). Therefore, ITMC has two advantages over other techniques that monitor NP phagocytosis: (1) there is no need to tag NPs with a fluorescent probe for intracellular detection, and (2) ITMC provides a continuous thermal record of the phagocytosis process (32). This study assessed the feasibility of using ITMC to monitor phagocytosis and investigated macrophage responses to different NP formulations and compared the reults with flow cytometry data.…”

Section: Introductionmentioning

confidence: 99%

Microcalorimetric Method to Assess Phagocytosis: Macrophage-Nanoparticle Interactions

AlHallak

Sarfraz

Azarmi

et al. 2010

AAPS J

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Abstract. This study evaluated the use of isothermal microcalorimetry (ITMC) to detect macrophagenanoparticle interactions. Four different nanoparticle (NP) formulations were prepared: uncoated poly (isobutyl cyanoacrylate) (PIBCA), polysorbate-80-coated PIBCA, gelatin, and mannosylated gelatin NPs. Changes in NP formulations were aimed to either enhance or decrease macrophage-NP interactions via phagocytosis. Alveolar macrophages were cultured on glass slabs and inserted in the ITMC instrument. Thermal activities of the macrophages alone and after titration of 100 μL of NP suspensions were compared. The relative interactive coefficients of macrophage-NP interactions were calculated using the heat exchange observed after NP titration. Control experiments were performed using cytochalasin B (Cyto B), a known phagocytosis inhibitor. The results of NP titration showed that the total thermal activity produced by macrophages changed according to the NP formulation. Mannosylated gelatin NPs were associated with the highest heat exchange, 75.4±7.5 J, and thus the highest relative interactive coefficient, 9,269±630 M-1. Polysorbate-80-coated NPs were associated with the lowest heat exchange, 15.2±3.4 J, and the lowest interactive coefficient, 890±120 M-1. Cyto B inhibited macrophage response to NPs, indicating a connection between the thermal activity recorded and NP phagocytosis. These results are in agreement with flow cytometry results. ITMC is a valuable tool to monitor the biological responses to nano-sized dosage forms such as NPs. Since the thermal activity of macrophage-NP interactions differed according to the type of NPs used, ITMC may provide a method to better understand phagocytosis and further the development of colloidal dosage forms.

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Section: Introductionmentioning

confidence: 99%

Microcalorimetric Method to Assess Phagocytosis: Macrophage-Nanoparticle Interactions

AlHallak

Sarfraz

Azarmi

et al. 2010

AAPS J

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Inhalable nanoparticles in lung cancer treatment; efficacy, safety, distribution and nanoparticle-macrophage interactions

Al-Hallak¹

2012

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A microcalorimetric method to study the activation of murine peritoneal macrophages

Cited by 2 publications

References 9 publications

Microcalorimetric Method to Assess Phagocytosis: Macrophage-Nanoparticle Interactions

Microcalorimetric Method to Assess Phagocytosis: Macrophage-Nanoparticle Interactions

Inhalable nanoparticles in lung cancer treatment; efficacy, safety, distribution and nanoparticle-macrophage interactions

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