A microfluidic cell culture platform for real-time cellular imaging

Hsieh, Chia‐Chun; Huang, Song Bin; Wu, Ping; Shieh, Dar Bin; Lee, Gwo‐Bin

doi:10.1007/s10544-009-9307-7

Cited by 30 publications

(23 citation statements)

References 30 publications

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“…Whilst in some cases some degree of stress has been found to induce growth and differentiation, 146 direct exposure to the flow rates generally required to supply sufficient nutrients is undesirable due to the damaging effect on the cells. Consequently, a wide range of approaches have been employed to protect cells from shear stress, including low flow rates, 43,48,102 changes in channel depth, 42 or geometry; 103,110 incorporation of microwells, 101 microchannels, 108,109 grooves, 41 micropillars, 66,67 or membranes 40,47,120,121 in or around the cell culture zone.…”

Section: Further Discussion and Concluding Remarksmentioning

confidence: 99%

“…2(C)) was described by Hsieh et al 67 The cell culture module (CCM) consisted of five reservoirs for medium or drugs, which were delivered to a thin micro-culture chamber (180 lm depth, designed for high-resolution cellular and sub-cellular imaging) via microchannels (500 lm wide initially and 5000 lm wide at the culture area) controlled by S-shape pneumatic micropumps and check valves. A temperature-control module maintained the cell culture environment at 37 C. The influence of shear stress was minimised by the low flow rate and a series of ring-shape pillars that encompassed the cell culture area.…”

Section: Integration and Multiplexingmentioning

confidence: 99%

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Microfluidic devices for cell cultivation and proliferation

et al. 2013

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Microfluidic technology provides precise, controlled-environment, cost-effective, compact, integrated, and high-throughput microsystems that are promising substitutes for conventional biological laboratory methods. In recent years, microfluidic cell culture devices have been used for applications such as tissue engineering, diagnostics, drug screening, immunology, cancer studies, stem cell proliferation and differentiation, and neurite guidance. Microfluidic technology allows dynamic cell culture in microperfusion systems to deliver continuous nutrient supplies for long term cell culture. It offers many opportunities to mimic the cell-cell and cell-extracellular matrix interactions of tissues by creating gradient concentrations of biochemical signals such as growth factors, chemokines, and hormones. Other applications of cell cultivation in microfluidic systems include high resolution cell patterning on a modified substrate with adhesive patterns and the reconstruction of complicated tissue architectures. In this review, recent advances in microfluidic platforms for cell culturing and proliferation, for both simple monolayer (2D) cell seeding processes and 3D configurations as accurate models of in vivo conditions, are examined.

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Section: Further Discussion and Concluding Remarksmentioning

confidence: 99%

Section: Integration and Multiplexingmentioning

confidence: 99%

Microfluidic devices for cell cultivation and proliferation

et al. 2013

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“…The microsystem itself is comparable to others in size and structure (Chang et al, 2003;Hsieh et al, 2009;Hung et al, 2005). It has a circular cultivation chamber, which is 3 mm in diameter, and several supply channels.…”

Section: Introductionmentioning

confidence: 88%

Cell cultures in microsystems: Biocompatibility aspects

Fischer

Steinert

Fröber

et al. 2010

Biotech & Bioengineering

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Bio-Micro-Electro-Mechanical Systems (BioMEMS) are a new tool in life sciences, supporting cell biology research by providing reproducible and miniaturized experimental platforms. In order to cultivate cells in such systems, appropriate microenvironmental conditions are required. Due to the multitude and variety of microbioreactors and cultivated cell types available, standardized cell handling methods and comprehensive biocompatibility data are sparse. The bioreactor developed at Ilmenau University of Technology features BioMEMS consisting of silicon, glass, and polymers, supplied by peripheral components. To verify the system's suitability for cell cultivation, it was necessary to prove whether materials and surfaces are biocompatible. Custom-tailored biocompatibility test procedures along with adequate cell seeding and handling methods had to be developed. According to this, proper positive and negative control samples had to be identified. The cultivation procedures were carried out using osteoblast-like murine fibroblasts (MC3T3-E1) and primary human osteoblasts (hOB). We could provide evidence that cultivation of these cells in our BioMEMS is feasible. In this context the relevant materials and the system's structure can be regarded as to be biocompatible. We could show that cell seeding and handling methods possess a strong impact on growth, development, and cellular activity of cell cultures in BioMEMS. Statistical biocompatibility data for the materials used is given.

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“…Nanoparticles can penetrate into capillaries and enter cells easily due to their nanoscale sizes (Wong et al 2011;Hsieh et al 2009). Furthermore, nanoparticles could preferably accumulate in tumor tissues with larger quantities than in normal tissues, due to the enhanced permeation and retention (EPR) effect of the cancerous tissues (Maeda et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Controlled delivery of hollow corn protein nanoparticles via non-toxic crosslinking: in vivo and drug loading study

Shen

et al. 2015

Biomed Microdevices

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In this research, controlled delivery of hollow nanoparticles from zein, the corn storage protein, to different organs of mice was achieved via crosslinking using citric acid, a non-toxic polycarboxylic acid derived from starch. Besides, crosslinking significantly enhanced water stability of nanoparticles while preserving their drug loading efficiency. Protein nanoparticles have been widely investigated as vehicles for delivery of therapeutics. However, protein nanoparticles were not stable in physiological conditions, easily cleared by mononuclear phagocyte system (MPS), and thus mainly accumulated and degraded in spleen and liver, the major MPS organs. Effective delivery to major non-MPS organs, such as kidney, was usually difficult to achieve, as well as long resident time of nanoparticles. In this research, hollow zein nanoparticles were chemically crosslinked with citric acid. Controlled delivery and prolonged accumulation of the nanoparticles in kidney, one major non-MPS organ, were achieved. The nanoparticles showed improved stability in aqueous environment at pH 7.4 without affecting the adsorption of 5-FU, a common anticancer drug. In summary, citric acid crosslinked hollow zein nanoparticles could be potential vehicles for controllable delivery of anticancer therapeutics.

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A microfluidic cell culture platform for real-time cellular imaging

Cited by 30 publications

References 30 publications

Microfluidic devices for cell cultivation and proliferation

Microfluidic devices for cell cultivation and proliferation

Cell cultures in microsystems: Biocompatibility aspects

Controlled delivery of hollow corn protein nanoparticles via non-toxic crosslinking: in vivo and drug loading study

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