A Microfluidic Chip for Cell Patterning Utilizing Paired Microwells and Protein Patterns

Tu, Chunlong; Huang, Bobo; Zhou, Jian; Liang, Yitao; Tian, Jian; Ji, Lin; Liang, Xiao; Ye, Xuesong

doi:10.3390/mi8010001

Cited by 38 publications

(27 citation statements)

References 46 publications

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“…This device was composed of one top PDMS channel, sandwiching a semi-porous polycarbonate membrane and a bottom PDMS channel, so that the flow and cells pass through them. Finally, the group of Xuesong Ye et al [ 82 ], in a very recent experiment, developed a microfluidic chip to pattern two cancer cell lines; HeLa and human gallbladder carcinoma cells (SGC-996) and were able to observe phenomena such as colony formation, cell migration, and cell proliferation. Firstly, PLL and Laminin proteins were printed with µCP and then a PDMS stamp, carrying paired microwells, was incubated on the substrate of the microfluidic chip ( Figure 11 ).…”

Section: Techniques and Methodsmentioning

confidence: 99%

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Methods of Micropatterning and Manipulation of Cells for Biomedical Applications

et al. 2017

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Micropatterning and manipulation of mammalian and bacterial cells are important in biomedical studies to perform in vitro assays and to evaluate biochemical processes accurately, establishing the basis for implementing biomedical microelectromechanical systems (bioMEMS), point-of-care (POC) devices, or organs-on-chips (OOC), which impact on neurological, oncological, dermatologic, or tissue engineering issues as part of personalized medicine. Cell patterning represents a crucial step in fundamental and applied biological studies in vitro, hence today there are a myriad of materials and techniques that allow one to immobilize and manipulate cells, imitating the 3D in vivo milieu. This review focuses on current physical cell patterning, plus chemical and a combination of them both that utilizes different materials and cutting-edge micro-nanofabrication methodologies.

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Section: Techniques and Methodsmentioning

confidence: 99%

“…On the right, a schema of the final microfluidic chip showing triangular microwells where cells are captured. Reproduced with permission from [ 82 ].…”

Section: Figurementioning

confidence: 99%

Methods of Micropatterning and Manipulation of Cells for Biomedical Applications

et al. 2017

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“…On the other hand, since SU-8 is photosensitive material, it is not as suitable for cell culture when compared with polystyrene. Surface treatment using a coating material or plasma treatment is needed to culture cells on the photoresist [14,26,27]. Since a porous membrane made of the photoresist is fragile, it is coated using poly D-lysine or collagen, and is not plasma treated [28,29].…”

Section: Cellular Tissue Cultured On An Mis Made Of Photoresist and Pmentioning

confidence: 99%

Micropatterning Method for Porous Materials Using the Difference of the Glass Transition Temperature between Exposed and Unexposed Areas of a Thick-Photoresist

et al. 2019

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A cell culture on a scaffold has the advantages of functionality and easy handling, because the geometry of the cellular tissue is controlled by designing the scaffold. To create complex cellular tissue, scaffolds should be complex two-dimensional (2D) and three-dimensional (3D) structures. However, it is difficult to fabricate a scaffold with a 2D and 3D structure because the shape, size, and fabrication processes of a 2D structure in creating a cell layer, and a 3D structure containing cells, are different. In this research, we propose a micropatterning method for porous materials using the difference of the glass transition temperature between exposed and unexposed areas of a thick-photoresist. Since the proposed method does not require a vacuum, high temperature, or high voltage, it can be used for fabricating various structures with a wide range of scales, regardless of the materials used. Additionally, the patterning area can be fabricated accurately by photolithography. To evaluate the proposed method, a membrane integrated scaffold (MIS) with a 2D porous membrane and 3D porous material was fabricated. The MIS had a porous membrane with a pore size of 4 μm or less, which was impermeable to cells, and a porous material which was capable of containing cells. By seeding HUVECs and HeLa cells on each side of the MIS, the cellular tissue was formed with the designed geometry.

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“…Initially used for the electronics industry, micropatterning now provides new tools for cell biology, and, combined with surface chemistry, it is particularly useful to study the interactions of cells with their microenvironment on different engineered surfaces [1,2]. Over the years, different micropatterning techniques of varying complexity have been developed, including microcontact printing, microfluidic patterning, UV-based deep etching and micro-stencils [3][4][5][6][7]. While there are advantages and drawbacks to each of these methods, they provide means to probe fundamental cellular functions, including cell adhesion and migration, cell polarity, cell shape dynamics, cytoskeletal rearrangement, and spatial coordination between cell and nuclear shape [1,[8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

A Micropatterning Strategy to Study Nuclear Mechanotransduction in Cells

2019

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Micropatterning techniques have been widely used in biology, particularly in studies involving cell adhesion and proliferation on different substrates. Cell micropatterning approaches are also increasingly employed as in vitro tools to investigate intracellular mechanotransduction processes. In this report, we examined how modulating cellular shapes on two-dimensional rectangular fibronectin micropatterns of different widths influences nuclear mechanotransduction mediated by emerin, a nuclear envelope protein implicated in Emery–Dreifuss muscular dystrophy (EDMD). Fibronectin microcontact printing was tested onto glass coverslips functionalized with three different silane reagents (hexamethyldisilazane (HMDS), (3-Aminopropyl)triethoxysilane (APTES) and (3-Glycidyloxypropyl)trimethoxysilane (GPTMS)) using a vapor-phase deposition method. We observed that HMDS provides the most reliable printing surface for cell micropatterning, notably because it forms a hydrophobic organosilane monolayer that favors the retainment of surface antifouling agents on the coverslips. We showed that, under specific mechanical cues, emerin-null human skin fibroblasts display a significantly more deformed nucleus than skin fibroblasts expressing wild type emerin, indicating that emerin plays a crucial role in nuclear adaptability to mechanical stresses. We further showed that proper nuclear responses to forces involve a significant relocation of emerin from the inner nuclear envelope towards the outer nuclear envelope and the endoplasmic reticulum membrane network. Cell micropatterning by fibronectin microcontact printing directly on HMDS-treated glass represents a simple approach to apply steady-state biophysical cues to cells and study their specific mechanobiology responses in vitro.

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A Microfluidic Chip for Cell Patterning Utilizing Paired Microwells and Protein Patterns

Cited by 38 publications

References 46 publications

Methods of Micropatterning and Manipulation of Cells for Biomedical Applications

Methods of Micropatterning and Manipulation of Cells for Biomedical Applications

Micropatterning Method for Porous Materials Using the Difference of the Glass Transition Temperature between Exposed and Unexposed Areas of a Thick-Photoresist

A Micropatterning Strategy to Study Nuclear Mechanotransduction in Cells

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