A Microfluidic Chip with Double-Slit Arrays for Enhanced Capture of Single Cells

Abstract

The application of microfluidic technology to manipulate cells or biological particles is becoming one of the rapidly growing areas, and various microarray trapping devices have recently been designed for high throughput single-cell analysis and manipulation. In this paper, we design a double-slit microfluidic chip for hydrodynamic cell trapping at the single-cell level, which maintains a high capture ability. The geometric effects on flow behaviour are investigated in detail for optimizing chip architecture, including the flow velocity, the fluid pressure, and the equivalent stress of cells. Based on the geometrical parameters optimized, the double-slit chip enhances the capture of HeLa cells and the drug experiment verifies the feasibility of the drug delivery.

Recent Development of Microfluidic Technology for Cell Trapping in Single Cell Analysis: A Review

“…The trapping efficiency was up to 70%. Similar to the design of Fan et al, Xu et al [53] developed a microfluidic device with double-slit arrays, as shown in Figure 4a. The microfluidic device consisted of an inlet reservoir, support and disperse pillars, micro-array, outlet channel and outlet reservoir.…”

“…It was found that the microstructure of type C was the most efficient in cell trapping, as shown in Figure 5a(ii). The blue bar and red bar represent the trapping efficiency Similar to the design of Fan et al, Xu et al [53] developed a microfluidic device with double-slit arrays, as shown in Figure 4a. The microfluidic device consisted of an inlet reservoir, support and disperse pillars, micro-array, outlet channel and outlet reservoir.…”

Non-invasive detection of bladder cancer via microfluidic immunoassay of the protein biomarker NMP22

“…The capture array is a critical component of a microfluidic immunoassay chip because its design and fabrication are key factors in the sensitivity of clinical detection and the performance of the assay. 14 The arrays of microwell, magnetic microsphere, and micropillar have been developed and investigated. However, each of them has limitations.…”