A microfluidic device enabling surface-enhanced Raman spectroscopy at chip-integrated multifunctional nanoporous membranes

Krafft, Benjamin; Panneerselvam, Rajapandiyan; Geißler, David; Belder, Detlev

doi:10.1007/s00216-019-02228-9

Cited by 23 publications

(14 citation statements)

References 45 publications

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“…During the experiments, ions can penetrate from the PBS filled extraction channel to the sample channel which is filled with tap water by diffusion and electromigration. As previously described, diffusion through the membrane is negligible (concerning the time scale of the whole experiment) and does not interfere with the measurement [30,32]. During the application of voltage, cations travel back to the extraction channel, whereas anions migrate towards the sample channel.…”

Section: Resultsmentioning

confidence: 99%

“…As most bacterial cells are in the range of a few micrometers, a membrane with a pore size of 0.2 μm was chosen as it allows fluidic transfer, whereas cells are retained. The microfluidic channels were fabricated in PDMS, Sylgard 184, Dow Corning, USA) according to established rapid prototyping and soft lithography processes which were described previously [30]. Briefly, a replica master was prepared by structuring a 60 μm thick layer of the negative photoresist SU-8 2050 (MicroChem, USA) on a clean 4-inch silicon wafer by common photolithography.…”

Section: Device Fabrication and Operationmentioning

confidence: 99%

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Microfluidic device for concentration and SERS‐based detection of bacteria in drinking water

et al. 2020

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There is a constant need for the development of easy-to-operate systems for the rapid and unambiguous identification of bacterial pathogens in drinking water without the requirement for time-consuming culture processes. In this study, we present a disposable and lowcost lab-on-a-chip device utilizing a nanoporous membrane, which connects two stacked perpendicular microfluidic channels. Whereas one of the channels supplies the sample, the second one attracts it by potential-driven forces. Surface-enhanced Raman spectrometry (SERS) is employed as a reliable detection method for bacteria identification. To gain the effect of surface enhancement, silver nanoparticles were added to the sample. The pores of the membrane act as a filter trapping the bodies of microorganisms as well as clusters of nanoparticles creating suitable conditions for sensitive SERS detection. Therein, we focused on the construction and characterization of the device performance. To demonstrate the functionality of the microfluidic chip, we analyzed common pathogens (Escherichia coli DH5α and Pseudomonas taiwanensis VLB120) from spiked tap water using the optimized experimental parameters. The obtained results confirmed our system to be promising for the construction of a disposable optical platform for reliable and rapid pathogen detection which couples their electrokinetic concentration on the integrated nanoporous membrane with SERS detection.

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Section: Resultsmentioning

confidence: 99%

Section: Device Fabrication and Operationmentioning

confidence: 99%

Microfluidic device for concentration and SERS‐based detection of bacteria in drinking water

et al. 2020

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“…A microfluidic implementation of SERS was proposed by Chang et al, who developed a detection device equipped with a membrane whose purpose was to concentrate a bacterial suspension and separate metabolites for the subsequent SERS aimed to the determination of antibiotic susceptibility [ 188 ]. In another study, Krafft et al set up a microfluidic chip equipped with a membrane, which fulfilled two functions at the same time, namely enriching the liquid sample and hosting the nanoparticles necessary for the SERS detection [ 189 ].…”

Section: Dynamic Investigation Techniquesmentioning

confidence: 99%

Membrane Fouling Phenomena in Microfluidic Systems: From Technical Challenges to Scientific Opportunities

2021

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The almost ubiquitous, though undesired, deposition and accumulation of suspended/dissolved matter on solid surfaces, known as fouling, represents a crucial issue strongly affecting the efficiency and sustainability of micro-scale reactors. Fouling becomes even more detrimental for all the applications that require the use of membrane separation units. As a matter of fact, membrane technology is a key route towards process intensification, having the potential to replace conventional separation procedures, with significant energy savings and reduced environmental impact, in a broad range of applications, from water purification to food and pharmaceutical industries. Despite all the research efforts so far, fouling still represents an unsolved problem. The complex interplay of physical and chemical mechanisms governing its evolution is indeed yet to be fully unraveled and the role played by foulants’ properties or operating conditions is an area of active research where microfluidics can play a fundamental role. The aim of this review is to explore fouling through microfluidic systems, assessing the fundamental interactions involved and how microfluidics enables the comprehension of the mechanisms characterizing the process. The main mathematical models describing the fouling stages will also be reviewed and their limitations discussed. Finally, the principal dynamic investigation techniques in which microfluidics represents a key tool will be discussed, analyzing their employment to study fouling.

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“…However, melamine is toxic and can cause many serious health issues, including reproduction damage, bladder infection, kidney stones, acute kidney failure, bladder cancer, and infant death [ 60 ]. The detection of melamine in milk samples (non-detectable) requires an extremely high resolution, and hence recent microfluidic devices for melamine analysis generally use high-sensitivity detection techniques such as electrochemistry (EC) [ 61 , 62 ], colorimetric AuNP sensing [ 63 , 64 ], surface-enhanced Raman scattering (SERS) [ 65 , 66 ], and immunosensor [ 67 , 68 ]. For example, Li et al [ 62 ] developed a microfluidic EC DNA-based sensor platform for continuous, real-time melamine detection in whole milk samples.…”

Section: Microfluidic Platforms For Milk Sample Analysismentioning

confidence: 99%

“…Krafft et al [ 66 ] developed a microfluidic lab-on-a-chip device combined with a SERS detector for the detection of melamine in whole milk samples. As shown in Figure 2 c, the device consisted of a nanoporous membrane designed to both enrich the sample through a selective electrokinetic filtering process and facilitate SERS through the use of a AgNP layer on its under surface.…”

Section: Microfluidic Platforms For Milk Sample Analysismentioning

confidence: 99%

Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

Lee

Kung

et al. 2021

Micromachines

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Milk is a necessity for human life. However, it is susceptible to contamination and adulteration. Microfluidic analysis devices have attracted significant attention for the high-throughput quality inspection and contaminant analysis of milk samples in recent years. This review describes the major proposals presented in the literature for the pretreatment, contaminant detection, and quality inspection of milk samples using microfluidic lab-on-a-chip and lab-on-paper platforms in the past five years. The review focuses on the sample separation, sample extraction, and sample preconcentration/amplification steps of the pretreatment process and the determination of aflatoxins, antibiotics, drugs, melamine, and foodborne pathogens in the detection process. Recent proposals for the general quality inspection of milk samples, including the viscosity and presence of adulteration, are also discussed. The review concludes with a brief perspective on the challenges facing the future development of microfluidic devices for the analysis of milk samples in the coming years.

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A microfluidic device enabling surface-enhanced Raman spectroscopy at chip-integrated multifunctional nanoporous membranes

Cited by 23 publications

References 45 publications

Microfluidic device for concentration and SERS‐based detection of bacteria in drinking water

Microfluidic device for concentration and SERS‐based detection of bacteria in drinking water

Membrane Fouling Phenomena in Microfluidic Systems: From Technical Challenges to Scientific Opportunities

Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk

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