A microfluidic device for label-free separation sensitivity enhancement of circulating tumor cells of various and similar size

Varmazyari, Vali; Ghafoorifard, Hassan; Habibiyan, Hamidreza; Ebrahimi, Marzieh; Ghafouri‐Fard, Soudeh

doi:10.1016/j.molliq.2021.118192

Cited by 14 publications

(12 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This paper investigates the performance of a proposed DEP device for three different configurations of 3D electrodes to separate MDA-MB-231 cancer cells from the different subtypes of WBCs with a cell viability approach. It should be noted that WBCs and CTCs are slightly larger than other blood components, so before separating WBCs from CTCs in the proposed system, they can be separated from other blood components using these two methods: (1) conventional cell sorting techniques such as centrifugation with density gradient, and (2) cell sorting based on microfluidic systems using deterministic lateral displacement (DLD) 36 , pinch flow fractionation (PFF) 37 , hydrodynamic filtration 38 , and inertial migration 39 mechanisms. In this section, various parameters such as cell trajectory, the electric field in the microchannel, DEP force applied to cells, and Joule heating are surveyed.…”

Section: Resultsmentioning

confidence: 99%

A dielectrophoresis-based microfluidic system having double-sided optimized 3D electrodes for label-free cancer cell separation with preserving cell viability

Varmazyari

Habibiyan

Ghafoorifard

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

Early detection of circulating tumor cells (CTCs) in a patient's blood is essential to accurate prognosis and effective cancer treatment monitoring. The methods used to detect and separate CTCs should have a high recovery rate and ensure cells viability for post-processing operations, such as cell culture and genetic analysis. In this paper, a novel dielectrophoresis (DEP)-based microfluidic system is presented for separating MDA-MB-231 cancer cells from various subtypes of WBCs with the practical cell viability approach. Three configurations for the sidewall electrodes are investigated to evaluate the separation performance. The simulation results based on the finite-element method show that semi-circular electrodes have the best performance with a recovery rate of nearly 95% under the same operational and geometric conditions. In this configuration, the maximum applied electric field (1.11 × 105 V/m) to separate MDA-MB-231 is lower than the threshold value for cell electroporation. Also, the Joule heating study in this configuration shows that the cells are not damaged in the fluid temperature gradient (equal to 1 K). We hope that such a complete and step-by-step design is suitable to achieve DEP-based applicable cell separation biochips.

show abstract

Section: Resultsmentioning

confidence: 99%

A dielectrophoresis-based microfluidic system having double-sided optimized 3D electrodes for label-free cancer cell separation with preserving cell viability

Varmazyari

Habibiyan

Ghafoorifard

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Leveraging these differences, the deterministic lateral displacement (DLD) method has emerged as a powerful tool for the separation, 64 Cell experiences two internal forces, pushing them either toward or away from the channel walls. 75,76 The disparity in these forces anchored CTCs in the center of the microchannel while pushing blood cells toward the walls. Building upon this principle, inertial spiral combining with DLD sorting has been proposed for high-accuracy CTC separation from diluted blood samples (Figure 2C).…”

Section: Deformability Based Ctc Isolationmentioning

confidence: 99%

“…Microfluidic‐based techniques leverage CTC hydrodynamics properties within the microchannels to drive the migration of cells, enabling the isolation of CTCs 64 . Cell experiences two internal forces, pushing them either toward or away from the channel walls 75,76 . The disparity in these forces anchored CTCs in the center of the microchannel while pushing blood cells toward the walls.…”

Section: Advanced Technologies For Isolating and Releasing Ctcsmentioning

confidence: 99%

Recent advances of liquid biopsy: Interdisciplinary strategies toward clinical decision‐making

Bao,

Min,

et al. 2023

Interdisciplinary Medicine

View full text Add to dashboard Cite

Liquid biopsy has emerged as a promising avenue for non‐invasive and rapid retrieval of pathological information from patient body fluids. Over the years, liquid biopsy has garnered significant attention for clinically treating cancer by selecting appropriate biomarkers such as circulating tumor cells (CTCs) and extracellular vesicles (EVs). Further integration of advanced technologies has facilitated the efficient capture of biomarkers for liquid biopsy, revolutionizing clinical decision‐making in the multiple processes and stages of cancer patients. Underscoring the intersection of different disciplines, this review provides a holistic summary of recent breakthroughs specifically designed for the capture and application of distinctive biomarkers to blend real‐world clinical decision‐making with material science. Firstly, we focus on the main principles of liquid biopsy and recent technologies that facilitate the capture and release of biomarkers (e.g., CTCs and EVs), leveraging their physicochemical properties. Then, the clinical applications of biomarkers are summarized, highlighting their potential for providing comprehensive clinical information. Later, the incorporation of machine learning is also emphasized for enhancing clinical applications and enabling deeper insights in the design of next‐generation platforms for specific biomarker isolation. Finally, future opportunities for clinical decision‐making are explored by combining advanced nanotechnologies and artificial intelligence, thereby offering inconceivable possibilities for improving patient care.

show abstract

“…Depending on the pillar size and spacing, cells with diameters less than the critical diameter move directly through the gaps between the micropillars, while cells bigger than the critical diameter bump into the micropillars and displace laterally to the next streamline. 39 A number of DLD devices have been investigated to isolate CTCs and CTC clusters from blood samples. 40 For example, Loutherback et al developed a DLD microfluidic device consisting of arrays of triangular-shaped micro-posts to increase throughput and reduce clogging.…”

Section: Hydrodynamic-based Techniquementioning

confidence: 99%

Section: Current Technologies For Ctc and Ctc Clusters Isolationmentioning

confidence: 99%

Advances in novel strategies for isolation, characterization, and analysis of CTCs and ctDNA

Yaghoubi Naei,

Bordhan,

Mirakhorli

et al. 2023

Ther Adv Med Oncol

View full text Add to dashboard Cite

Over the past decade, the detection and analysis of liquid biopsy biomarkers such as circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) have advanced significantly. They have received recognition for their clinical usefulness in detecting cancer at an early stage, monitoring disease, and evaluating treatment response. The emergence of liquid biopsy has been a helpful development, as it offers a minimally invasive, rapid, real-time monitoring, and possible alternative to traditional tissue biopsies. In resource-limited settings, the ideal platform for liquid biopsy should not only extract more CTCs or ctDNA from a minimal sample volume but also accurately represent the molecular heterogeneity of the patient’s disease. This review covers novel strategies and advancements in CTC and ctDNA-based liquid biopsy platforms, including microfluidic applications and comprehensive analysis of molecular complexity. We discuss these systems’ operational principles and performance efficiencies, as well as future opportunities and challenges for their implementation in clinical settings. In addition, we emphasize the importance of integrated platforms that incorporate machine learning and artificial intelligence in accurate liquid biopsy detection systems, which can greatly improve cancer management and enable precision diagnostics.

show abstract

A microfluidic device for label-free separation sensitivity enhancement of circulating tumor cells of various and similar size

Cited by 14 publications

References 59 publications

A dielectrophoresis-based microfluidic system having double-sided optimized 3D electrodes for label-free cancer cell separation with preserving cell viability

A dielectrophoresis-based microfluidic system having double-sided optimized 3D electrodes for label-free cancer cell separation with preserving cell viability

Recent advances of liquid biopsy: Interdisciplinary strategies toward clinical decision‐making

Advances in novel strategies for isolation, characterization, and analysis of CTCs and ctDNA

Contact Info

Product

Resources

About