A microfluidic device for simultaneous electrical and mechanical measurements on single cells

Chen, Jian; Zheng, Yi; Tan, Qingyuan; Zhang, Yan Liang; Li, Jason; Geddie, William R.; Jewett, Michael A.S.; Sun, Yu

doi:10.1063/1.3571530

Cited by 85 publications

(63 citation statements)

References 66 publications

(78 reference statements)

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“…We developed a microfluidic device for single-cell electrical and mechanical characterization using impedance spectroscopy and micropipette aspiration. 82 Cellular deformation was recorded as a function of increasing pressure, while cellular impedance was measured via two Ag/AgCl electrodes inserted into the culture medium. With pipette aspiration and equivalent circuit models, both mechanical properties and dielectric properties of single cells were quantified.…”

Section: Aspiration-induced Deformationmentioning

confidence: 99%

Recent advances in microfluidic techniques for single-cell biophysical characterization

et al. 2013

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Section: Aspiration-induced Deformationmentioning

confidence: 99%

Recent advances in microfluidic techniques for single-cell biophysical characterization

et al. 2013

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“…According to the patch-clamp study, when the cell is aspired onto the mouth of the pipette, the electric effect on the whole feedback circuit can be modeled as an equivalent circuit as shown in Figure 7B [9]. When the direct voltage is applied to the feedback circuit in Figure 7 A, the current just goes through the external R, Rpipette and R1 eak , and is blocked out by the capacitor.…”

Section: B the Overall Operation Precedure Of The Systemmentioning

confidence: 99%

Automated micropipette aspiration of cell using resistance-based voltage feedback control

Dong

Wang

et al. 2013

The 7th IEEE International Conference on Nano/Molecular Medicine and Engineering

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Abstract-This paper presents an automation operation system for the single cell aspiration and placement with high throughput. In the system, a glass capillary micropipette is utilized to move above the microwell substrate which contains living cells, select and aspire individual cells of interest, transfer and release them to target locations. With the microwell biochip coordinate system, the micropipette can be positioned precisely and moves automatically to target microwell with path planning and control. With the visual feedback, living cells of interest are selected and then the micropipette is moved to approach to the cells for aspiration. The cell-resistance based voltage feedback is established for cell aspiration and placement control. The goal of this work is to realize the automated and efficient operation of the cells in the microwell array including aspiration, transfer and placement.

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“…To further mimicking the in vivo microcirculation and the role of capillaries, a number of works described the cell deformation using gap shape constrictions [53]. Squeezing zone allows for deformation have been tested on RBC [19,54], leukocytes [55], cancer [56] and osteoblasts cells [57]. Parameters like cell transit time through constrictions, transit or passage time, entrance time during squeezing and transit velocity were used for cell deformation measurements.…”

Section: Constricted Geometrymentioning

confidence: 99%

Trends in characterizing single cell's stiffness properties

Ahmad

2014

Micro and Nano Syst Lett

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Biophysical properties of living cells such as mechanical and chemical have been proven to play important roles in regulations of various biological activities including disease progression both at the cellular and molecular levels. In the past decades, a number of research tools have been developed to provide better understanding towards cell's biophysical states. This growing interest was supported by the emergence of researches focusing on single cell analysis (SCA) which serves as a platform enabling various experimentation works to be carried out. In this context, various techniques have been developed for single cell's mechanical characterization to improve robustness, accuracy and operational flexibility. The generic solution varies from traditional approach, microelectromechanical system (MEMS) and microfluidic. This paper presents a review of progress and developments in the field of single cell mechanical properties specifically discussing on stiffness characterizations. An analytical comparison of the reviewed solutions is presented, and the advantages and disadvantages of different techniques are compared.

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A microfluidic device for simultaneous electrical and mechanical measurements on single cells

Cited by 85 publications

References 66 publications

Recent advances in microfluidic techniques for single-cell biophysical characterization

Recent advances in microfluidic techniques for single-cell biophysical characterization

Automated micropipette aspiration of cell using resistance-based voltage feedback control

Trends in characterizing single cell's stiffness properties

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