A microfluidic platform for trapping, releasing and super-resolution imaging of single cells

Zhou, Ying; Basu, Srinjan; Wohlfahrt, Kai J.; Lee, Steven F.; Klenerman, David; Laue, Ernest D.; Seshia, Ashwin A.

doi:10.1016/j.snb.2016.03.131

Cited by 57 publications

(50 citation statements)

References 23 publications

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“…The trapping and transport of microscopic entities via hydrodynamic flow is an emergent field of research that could lead to novel and exciting developments in lab on a chip devices, such as the controlled release and site specific delivery of chemical or biological cargos. In microfluidic systems, where pressure fields are used to displace nanoliter volumes of reagent in 100 m m wide channels, the trapping, assembly and positioning of microspheres via hydrodynamic flow has been demonstrated in different works [1][2][3][4][5][6]. The time reversal nature of fluid flow at low Reynolds (Re) number [7] allows for realizing precise single particle operations at the microscale, since inverting the fluid current does not lead to the formation of swirls or turbulence that can randomize the motion of the dispersed particles.…”

Section: Introductionmentioning

confidence: 99%

Propulsion and hydrodynamic particle transport of magnetically twisted colloidal ribbons

et al. 2017

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We describe a method to trap, transport and release microscopic particles in a viscous fluid using the hydrodynamic flow field generated by a magnetically propelled colloidal ribbon. The ribbon is composed of ferromagnetic microellipsoids that arrange with their long axis parallel to each other, a configuration that is energetically favorable due to their permanent magnetic moments. We use an external precessing magnetic field to torque the anisotropic particles forming the ribbon, and to induce propulsion of the entire structure due to the hydrodynamic coupling with the close substrate. The propulsion speed of the ribbon can be controlled by varying the driving frequency, or the amplitude of the precessing field. The latter parameter is also used to reduce the average inter particle distance and to induce the twisting of the ribbon due to the increase in the attraction between the rotating ellipsoids. Furthermore, non magnetic particles are attracted or repelled with the hydrodynamic flow field generated by the propelling ribbon. The proposed method may be used in channel free microfluidic applications, where the precise trapping and transport of functionalized particles via non invasive magnetic fields is required.

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Section: Introductionmentioning

confidence: 99%

Propulsion and hydrodynamic particle transport of magnetically twisted colloidal ribbons

et al. 2017

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“…Microfluidics is increasingly being used in many areas of biotechnology and chemistry [1][2][3][4][5] to achieve reduced consumption of reagents /analytes, improved performance, low costs of fabrications and decrease time of analyses, and also, among other advantages, to obtain miniaturization and integration of fluidic components, developed as well known concept of "lab on a chip" [3,4,5].…”

Section: Introductionmentioning

confidence: 99%

Design of Microfluidic Device and Measurements of MPWM for Single Cell /Particle Manipulation

Ardeleanu

Mihai

Vidu

et al. 2019

Scientific Bulletin of Valahia University - Materials and Mechanics

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A microfluidic device designated for measurement of fluidic flows with different viscosity, necessary within trapping/realising of cells/particles system has been developed. We use a new concept as Microfluidic Pulse Width Modulation (MPWM) for controlling transport of a single cell/particle. The image processing helped the nano-hydraulic volumes/flow rates measurement, through tracking inovative methods with the purpose to build a flow sensor. The device open an unique opportunitie for single cell study with applications in biomedical devices, tools for biochemistry or analytical systems.

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“…As the cells in microwell arrays are only fixed in their position by gravity, continued perfusion of medium causes the cells to be washed out of the microfluidic device. To address this issue, cell traps consisting of several individual micropillars have been devised . Here, the cell spheroids are loaded into the traps through the flow of medium and later fixed in place with the same mechanism.…”

Section: Introductionmentioning

confidence: 99%

Micropillar‐based microfluidic device to regulate neurite networks of uniform‐sized neurospheres

et al. 2018

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The inability of neurons to undergo mitosis renders damage to the central or peripheral nervous system. Neural stem cell therapy could provide a path for treating the neurodegenerative diseases. However, reliable and simple tools for the developing and testing neural stem cell therapy are still required. Here, we show the development of a micropillar-based microfluidic device to trap the uniform-sized neurospheres. The neurospheres trapped within micropillar arrays were largely differentiated into neuronal cells, and their neurite networks were observed in the microfluidic device. Compared to conventional cultures on glass slides, the neurite networks generated with this method have a higher reproducibility. Furthermore, we demonstrated the effect of thapsigargin on the neurite networks in the microfluidic device, demonstrating that neural networks exposed to thapsigargin were largely diminished and disconnected from each other. Therefore, this micropillar-based microfluidic device could be a potential tool for screening of neurotoxins.

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A microfluidic platform for trapping, releasing and super-resolution imaging of single cells

Cited by 57 publications

References 23 publications

Propulsion and hydrodynamic particle transport of magnetically twisted colloidal ribbons

Propulsion and hydrodynamic particle transport of magnetically twisted colloidal ribbons

Design of Microfluidic Device and Measurements of MPWM for Single Cell /Particle Manipulation

Micropillar‐based microfluidic device to regulate neurite networks of uniform‐sized neurospheres

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