A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films

Hampe, Alexander E; Li, Zidong; Sethi, Sunjay; Lein, Pamela J.; Şeker, Erkin

doi:10.3390/nano8070452

Cited by 9 publications

(4 citation statements)

References 39 publications

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“…Nanotopography has been reported to guide complex biological processes including endocytosis, cell adhesion, cell alignment, cell proliferation, muscle maturation, neurogenesis, − and osteogenesis . Possible signaling pathways that enable the interaction between cells and nanotopographical cues have been proposed, including integrin-activated focal adhesion kinase, Rho-Rac-Myosin, and sphingosine-1-phosphate signaling .…”

mentioning

confidence: 99%

Nanostructured Architectures Promote the Mesenchymal–Epithelial Transition for Invasive Cells

et al. 2020

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Dynamic modulation of cellular phenotypes between the epithelial and mesenchymal statesthe epithelial–mesenchymal transition (EMT) and mesenchymal–epithelial transition (MET)plays an important role in cancer progression. Nanoscale topography of culture substrates is known to affect the migration and EMT of cancer cells. However, existing platforms heavily rely on simple geometries such as grooved lines or cylindrical post arrays, which may oversimplify the complex interaction between cells and nanotopography in vivo. Here, we use electrodeposition to construct finely controlled surfaces with biomimetic fractal nanostructures as a means of examining the roles of nanotopography during the EMT/MET process. We found that nanostructures in the size range of 100 to 500 nm significantly promote MET for invasive breast and prostate cancer cells. The “METed” cells acquired distinct expression of epithelial and mesenchymal markers, displayed perturbed morphologies, and exhibited diminished migration and invasion, even after the removal of a nanotopographical stimulus. The phosphorylation of GSK-3 was decreased, which further tuned the expression of Snail and modulated the EMT/MET process. Our findings suggest that invasive cancer cells respond to the geometries and dimensions of complex nanostructured architectures.

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confidence: 99%

Nanostructured Architectures Promote the Mesenchymal–Epithelial Transition for Invasive Cells

et al. 2020

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“…We suggest that the decrease in glial cell density brought about by NP-structured substrates may be ascribed to mechanisms related to the cell focal contact modulation by the topography and related mechanotransduction processes. [29][30][31][32] In addition, other features such as the reduced apparent stiffness of the nano-topographies may induce this cellular response. [33] Furthermore, a reduced glial cell proliferation may have been favored by the suspended configuration these cultures adopt in the high aspect ratio topography.…”

Section: Topography Fabrication and Characterizationmentioning

confidence: 99%

Polystyrene Nanopillars with Inbuilt Carbon Nanotubes Enable Synaptic Modulation and Stimulation in Interfaced Neuronal Networks

Calaresu

Hernández

Rauti

et al. 2021

Adv Materials Inter

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The use of nanostructured materials and nanosized‐topographies has the potential to impact the performance of implantable biodevices, including neural interfaces, enhancing their sensitivity and selectivity, while reducing tissue reactivity. As a result, current trends in biosensor technology require the effective ability to improve devices with controlled nanostructures. Nanoimprint lithography to pattern surfaces with high‐density and high aspect ratio nanopillars (NPs) made of polystyrene (PS‐NP, insulating), or of a polystyrene/carbon‐nanotube nanocomposite (PS‐CNT‐NP, electrically conductive) are exploited. Both substrates are challenged with cultured primary neurons. They are demonstrated to support the development of suspended synaptic networks at the NPs’ interfaces characterized by a reduction in proliferating neuroglia, and a boost in neuronal emergent electrical activity when compared to flat controls. The authors successfully exploit their conductive PS‐CNT‐NPs to stimulate cultured cells electrically. The ability of both nanostructured surfaces to interface tissue explants isolated from the mouse spinal cord is then tested. The integration of the neuronal circuits with the NP topology, the suspended nature of the cultured networks, the reduced neuroglia formation, and the higher network activity together with the ability to deliver electrical stimuli via PS‐CNT‐NP reveal such platforms as promising designs to implement on neuro‐prosthetic or neurostimulation devices.

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“…In order to overcome the structure weakness and leading agents in Pt black, lead-free Pt grey was invented and improved in [18,19] and used for nanostructured iridium oxide/Pt grey coating [20]. Alternatively, impedance can be reduced by increasing the surface area by introducing 3D topological nanostructures such as nanowires [21], nanotubes [22], nanopillars [23,24], and nanoporous electrodes [25][26][27][28][29]. Currently, Platinum nanograss (Ptng) has been demonstrated to increase the electrode roughness and reduce the electrode impedance at significantly reduced costs and effort compared to previously described methods [30].…”

Section: Introductionmentioning

confidence: 99%

Superior galvanostatic electrochemical deposition of platinum nanograss provides high performance planar microelectrodes for in vitro neural recording

2021

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Objective. Platinum nanograss (Ptng) has been demonstrated as an excellent coating to increase the electrode roughness and reduce the impedance of microelectrodes for neural recording. However, the optimisation of the original potentiostatic electrochemical deposition (PSED) method has been performed by the original group only and no in vitro validation of functionality was reported. Approach. This study firstly reinvestigates the use of the PSED method for Ptng coating at different charge densities which highlights non-uniformities in the edges of the microelectrodes for increasing deposition charge densities, leading to a decreased impedance which is in fact an artefact. We then introduce a novel Ptng fabrication method of galvanostatic electrochemical deposition (GSED). Main results. We demonstrate that the GSED deposition method also significantly reduces the electrode impedance, raises the charge storage capacity and provides a significantly more planar electrode surface in comparison to the PSED method with negligible edge effects. In addition, we demonstrate how high-quality neural recordings were performed, for the first time, using the Ptng GSED deposition microelectrodes from human hNT neurons and how spiking and bursting were observed. Significance. Thus, the GSED Ptng deposition method presented here provides an alternative method of microelectrode fabrication for neural applications with excellent impedance and planarity of surface.

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A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films

Cited by 9 publications

References 39 publications

Nanostructured Architectures Promote the Mesenchymal–Epithelial Transition for Invasive Cells

Nanostructured Architectures Promote the Mesenchymal–Epithelial Transition for Invasive Cells

Polystyrene Nanopillars with Inbuilt Carbon Nanotubes Enable Synaptic Modulation and Stimulation in Interfaced Neuronal Networks

Superior galvanostatic electrochemical deposition of platinum nanograss provides high performance planar microelectrodes for in vitro neural recording

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