A Microfluidic Sensor for Continuous, in Situ Surface Charge Measurement of Single Cells

Ni, Liwei; Shaik, Rubia; Xu, Ruiting; Zhang, Ge; Zhe, Jiang

doi:10.1021/acssensors.9b02411

Cited by 14 publications

(11 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to the different nature of biomolecules (e.g., dimensions, surface charges, mobility, etc.) [ 6 , 7 , 8 , 9 ], various detection methods have been developed. Commercially available instruments for biomolecular detection include surface-enhanced Raman spectroscopy (SERS) [ 10 , 11 ], surface plasmon resonance (SPR) [ 12 , 13 ], and gas chromatography-mass spectroscopy (GC-MS) [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles

Ouyang

Chen

et al. 2023

Biosensors

Self Cite

View full text Add to dashboard Cite

The fast, accurate detection of biomolecules, ranging from nucleic acids and small molecules to proteins and cellular secretions, plays an essential role in various biomedical applications. These include disease diagnostics and prognostics, environmental monitoring, public health, and food safety. Aptamer recognition (DNA or RNA) has gained extensive attention for biomolecular detection due to its high selectivity, affinity, reproducibility, and robustness. Concurrently, biosensing with nanoparticles has been widely used for its high carrier capacity, stability and feasibility of incorporating optical and catalytic activity, and enhanced diffusivity. Biosensors based on aptamers and nanoparticles utilize the combination of their advantages and have become a promising technology for detecting of a wide variety of biomolecules with high sensitivity, reliability, specificity, and detection speed. Via various sensing mechanisms, target biomolecules have been quantified in terms of optical (e.g., colorimetric and fluorometric), magnetic, and electrical signals. In this review, we summarize the recent advances in and compare different aptamer–nanoparticle-based biosensors by nanoparticle types and detection mechanisms. We also share our views on the highlights and challenges of the different nanoparticle-aptamer-based biosensors.

show abstract

Section: Introductionmentioning

confidence: 99%

Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles

Ouyang

Chen

et al. 2023

Biosensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…There has been significant recent progress in single cell analysis due to the importance of cellular heterogeneity in drug discovery, disease diagnosis, and therapy design. − The complex nature of cell-to-cell heterogeneity has motivated the development of tools for single cell analysis, including patch clamping, , in situ fluorescence hybridization, , flow cytometry, , immunospot assays, microfluidics, − single cell sequencing, , etc. These methods provide valuable information on basic biological processes in living cells, which promotes our understanding of cellular heterogeneity.…”

Section: Introductionmentioning

confidence: 99%

Specially Resolved Single Living Cell Perfusion and Targeted Fluorescence Labeling Based on Nanopipettes

Wang

Zhou

et al. 2022

Anal. Chem.

View full text Add to dashboard Cite

Targeted delivery and labeling of single living cells in heterogeneous cell populations are of great importance to understand the molecular biology and physiological functions of individual cells. However, it remains challenging to perfuse fluorescence markers into single living cells with high spatial and temporal resolution without interfering neighboring cells. Here, we report a single cell perfusion and fluorescence labeling strategy based on nanoscale glass nanopipettes. With the nanoscale tip hole of 100 nm, the use of nanopipettes allows special perfusion and high-resolution fluorescence labeling of different subcellular regions in single cells of interest. The dynamic of various fluorescent probes has been studied to exemplify the feasibility of nanopipette-dependent targeted delivery. According to experimental results, the cytoplasm labeling of Sulfo-Cyanine5 and fluorescein isothiocyanate is mainly based on the Brownian movement due to the dyes themselves and does not have a targeting ability, while the nucleus labeling of 4′,6-diamidino-2-phenylindole (DAPI) is originated from the adsorption between DAPI and DNA in the nucleus. From the finite element simulation, the precise manipulation of intracellular delivery is realized by controlling the electro-osmotic flow inside the nanopipettes, and the different delivery modes between nontargeting dyes and nucleus-targeting dyes were compared, showcasing the valuable ability of nanopipette-based method for the analysis of specially defined subcellular regions and the potential applications for single cell surgery, subcellular manipulation, and gene delivery.

show abstract

“…However, this method usually detects the motions of a group of cells; it is challenging to identify zeta potential for single cells [ 9 ], not to mention the surface charge distribution on a cell. Recently Ni et al developed a microfluidic sensor based on resistive pulse sensing to assess single cells’ surface charge and sizes [ 10 ]. However, it still cannot measure the cell surface charge distribution.…”

Section: Introductionmentioning

confidence: 99%

Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle

Ouyang

Shaik

et al. 2021

Cells

Self Cite

View full text Add to dashboard Cite

Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.

show abstract

A Microfluidic Sensor for Continuous, in Situ Surface Charge Measurement of Single Cells

Cited by 14 publications

References 46 publications

Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles

Recent Advances in Biomolecular Detection Based on Aptamers and Nanoparticles

Specially Resolved Single Living Cell Perfusion and Targeted Fluorescence Labeling Based on Nanopipettes

Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle

Contact Info

Product

Resources

About