A Microfluidic System to Evaluate Intestinal Absorption

Imura, Yoshimi; Asano, Yasuyuki; Sato, Katsuaki; Yoshimura, Etsuro

doi:10.2116/analsci.25.1403

Cited by 149 publications

(141 citation statements)

References 16 publications

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“…Although Imura et al 18 reported a bubble trap made of silicone tubes, the bubble trap could not trap all of the bubbles well. A lot of air bubbles appeared in a silicone tube, which was filled with a culture medium and then incubated in a CO2 incubator overnight (Fig.…”

Section: Resultsmentioning

confidence: 98%

Fluidic Culture and Analysis of Pulmonary Artery Smooth Muscle Cells for the Study of Pulmonary Hypertension

et al. 2016

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There is an urgent need to develop novel in-vitro models to mimic the disease conditions in pulmonary hypertension (PH). We developed a microfluidic cell culture device for PH studies that withstood high shear stress. Techniques were also developed for cell recovery from the microchannel and mRNA isolation from the collected cells. Using this device, we found that shear stress caused a 7.5-fold increase in the transcription levels of a PH-related molecule, Cyclin D1.

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Section: Resultsmentioning

confidence: 98%

Fluidic Culture and Analysis of Pulmonary Artery Smooth Muscle Cells for the Study of Pulmonary Hypertension

et al. 2016

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“…S1), there could be a difference in the kinetics of cell growth due to the different culture membranes, but after cells reach confluency and begin to form the barrier, TEER values become more uniform between the parallels and reflect good barrier properties. Higher Lucifer yellow permeability has been described for the same cell type cultured in a microfluidic chamber, but that system did not allow measurement of TEER [30]. Note that Caco-2 cells in a gut-on-a-chip model have reached tighter barrier properties when co-cultured with bacteria and/or immune cells, and exposed to low shear stress and cyclic strain to mimic peristaltic motion [4,32].…”

Section: Intestinal Modelmentioning

confidence: 99%

“…There are several setups built for similar purposes. Most of them consist of two channels, either parallel [30] or perpendicular to each other [19,31], with a relatively small overlapping area. The arrangement of our channels enables a much larger overlapping area (ca.…”

Section: Chip Structure and Assemblymentioning

confidence: 99%

A versatile lab-on-a-chip tool for modeling biological barriers

Walter

Valkai

Kincses

et al. 2016

Sensors and Actuators B: Chemical

148

156

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Keywords: Microfluidics Lung and intestinal epithelial cells Endothelial cells Blood-brain barrier PermeabilityTrans-epithelial/endothelial electric resistance a b s t r a c t Models of biological barriers are important to study physiological functions, transport mechanisms, drug delivery and pathologies. However, there are only a few integrated biochips which are able to monitor several of the crucial parameters of cell-culture-based barrier models. The aim of this study was to design and manufacture a simple but versatile device, which allows a complex investigation of barrier functions. The following functions and measurements are enabled simultaneously: co-culture of 2 or 3 types of cells; flow of culture medium; visualization of the entire cell layer by microscopy; real-time transcellular electrical resistance monitoring; permeability measurements. To this end, a poly(dimethylsiloxane)-based biochip with integrated transparent gold electrodes and with a possibility to connect to a peristaltic pump was built. Unlike previous systems, the structure of the device allowed a constant visual observation of cell growth over the whole membrane surface. Morphological characterization of the layers was also accomplished by immunohistochemical staining. The chip was applied to monitor and characterize models of the intestinal and lung epithelial barriers, and the blood-brain barrier. The models were established using human Caco-2 intestinal and A549 lung epithelial cell lines, hCMEC/D3 human brain endothelial cell line and primary rat brain endothelial cells co-cultured with primary astrocytes and brain pericytes. This triple primary co-culture blood-brain barrier model was assembled on a lab-on-a-chip device and investigated under fluid flow for the first time. Such a versatile tool is expected to facilitate the kinetic investigation of various biological barriers.

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“…Development of these micro engineering approaches has opened entirely new possibilities to create in vitro models that reconstitute more complex 3D organ level structures and to integrate crucial dynamic mechanical cues as well as chemical signals. There have been different kinds of tissue or organ chip models for specific research (Figure 6), such as liver-on-a-chip, [96] kidney-on-a-chip, [97,98] gut-on-a-chip, [99,100] lung-on-a-chip, [101,102] heart-on-a-chip [103] and vessel-on-a-chip [104].…”

Section: Microfluidic Applications In Cell Biologymentioning

confidence: 99%

Development and Applications of Microfluidic Devices for Cell Culture in Cell Biology

Yi¹,

Lin²

2016

Mol Biol

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Development of microfluidic culture technology combined with tissue engineering catalyzes the progress in study of cell biology. These tools will promote the understanding of physiological and pathological changes. Cancer cells and stem cells are sensitive to their surroundings, thus could be better explored by controllable microfluidic devices. In this review, we describe the ways to control cell microenvironment and explain how the influencing factors influence cellular behaviors, then present microfluidic-chip-based exemplary applications for cancer models and stem cell differentiation.

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A Microfluidic System to Evaluate Intestinal Absorption

Cited by 149 publications

References 16 publications

Fluidic Culture and Analysis of Pulmonary Artery Smooth Muscle Cells for the Study of Pulmonary Hypertension

Fluidic Culture and Analysis of Pulmonary Artery Smooth Muscle Cells for the Study of Pulmonary Hypertension

A versatile lab-on-a-chip tool for modeling biological barriers

Development and Applications of Microfluidic Devices for Cell Culture in Cell Biology

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