A Micromechanics Model for Turgor Pressure of Arabidopsis thaliana Protoplast

Phytagel media were evaluated as systems to mechanically impede roots of A. thaliana. Studying mechanical properties of Phytagel and exploring the root response to mechanical stimulation can facilitate plant culture and plant development. Breaking strengths of 0.5-2.0% phytagel media were tested by uniaxial compression test. Different phytagel concentrations were set to alter the strength of layers in growth medium. Negative correlations were observed between root length, straightness and medium strength. When roots elongated through soft upper-layer (0.6%), penetration ratio decreased with the increase of lower-layer strength (0.6-1.2%) and all roots couldn't penetrate into lower-layer with concentration ≥1.2%. Roots could grow into soft lower-layer (0.6%) from hard upper-layer (0.6-1.2%), with decreased penetration ratio. When roots grew in soft lower-layer, the growth rate linked with upper-layer strength increased to peak. Roots penetration capability into 1.2% lower-layer was improved by growing plants through moderate layer inserted between soft and hard layer, and roots in 0.8% moderate medium have a significant higher penetration ratio than that in 1.0%. It was concluded that the Phytagel systems studied were suitable for studying the effect of mechanical impedance on the elongation of A. thaliana roots. The medium strength affected root penetration significantly and acclimation can improve root penetration capability.

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A root penetration model of Arabidopsis thaliana in phytagel medium with different strength

Yan

Wang

Zhou

2017

J Plant Res

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The study on mechanical properties of Phytagel medium

Zhou

Yan

et al. 2019

IOP Conf. Ser.: Earth Environ. Sci.

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Background: The mechanical properties of plant culture medium such as Phytagel affect plant growth and development. Given that the mechanical properties of Phytagel medium are vital for biomechanical experiments designing, a systematic study on mechanical properties of Phytagel medium with different concentrations were carried out here to better understand the response of plant to mechanical stimulation. Results: Uniaxial compression test was conducted for the mechanical strength and Young’s modulus. The variation of concentrations of media results in different mechanical strength. The linear-regression analysis of the breaking load shows that there is a lack of fit of the linear regression model to the observed data points for all these Phytagel concentrations (R2 = 0.9708). The spline regression model, however, fits well to the Young’s modulus for Phytagel medium data. The rheological measurements from the oscillation tests (frequency sweep from 0.1 to 20 Hz at 1% strain) indicate that increasing the Phytagel concentrations results in a stiffer structure at 0.5%-1.2%. Conclusions: Although the biological effects of Phytagel on plant along with mechanical power are expected to check, this contribution provides a useful reference in biomechanical experiments to choose the best Phytagel concentration for the culture of plants and tissues.

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A Micromechanics Model for Turgor Pressure of Arabidopsis thaliana Protoplast

Cited by 2 publications

References 16 publications

A root penetration model of Arabidopsis thaliana in phytagel medium with different strength

A root penetration model of Arabidopsis thaliana in phytagel medium with different strength

The study on mechanical properties of Phytagel medium

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