A micromethod for fractionation of lipids by silicic acid chromatography

Lis, Elaine W.; Tinoco, J.; Okey, Ruth

doi:10.1016/0003-2697(61)90058-6

Cited by 77 publications

(25 citation statements)

References 6 publications

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“…The lipid fraction was taken to dryness and dissolved in n-hexane-diethyl ether (99:1), and an aliquot (10 mg) was placed on a silicic acid column for separation. Separation was achieved by modification of the methods described by Lis et al (12) and Rouser et al (18). Five fractions were obtained from the following series of elutions: 15 ml of n-hexane-diethyl ether (99:1); 15 ml of n-hexanediethyl ether (96:4); 15 ml of n-hexane-diethyl ether (50:50); 75 ml of acetone; and 15 ml of methanol; hereafter these are referred to as fractions 1, 2, 3, 4, and 5, respectively.…”

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confidence: 99%

Effect of Growth Temperature on the Lipid Composition of Cyanidium caldarium

Kleinschmidt

McMahon

1970

Plant Physiol.

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Cyanidium caldarium is a thermophilic eucaryote which can grow at temperatures below 20 C and has been reported growing at temperatures up to 56 C (5). This acid-tolerant and heattolerant unicellular alga is a regular member of the microflora found in acidic hot springs throughout the world (2,4). Electron microscopic investigations show a highly differentiated cell containing: a thick cell wall, plasmalemma, ground matrix, endoplasmic reticulum, chloroplast, mitochondria, nucleus, and vacuoles (14). Cell division occurs by formation of four daughter cells within the mother cell wall, and after rupturing the four daughter cells are released. These morphological characteristics make it quite suitable for studying the effect of temperature on lipid composition and its possible role in the molecular mechanism of thermophily.In order to develop and function properly, organisms must be capable of adjusting to their environment. Of the environmental factors, temperature is an important parameter determining the lethal ranges of survival. Since temperature affects all biological reactions, the over-all effect of temperature is rather complex; therefore, this investigation is limited to a narrower set of biological reactions: lipid metabolism.It has been well documented that an increase in temperature causes a corresponding decrease in the amount of unsaturated fatty acids (3,6, 8,10,13). Farkas and Herodek (6)

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confidence: 99%

Effect of Growth Temperature on the Lipid Composition of Cyanidium caldarium

Kleinschmidt

McMahon

1970

Plant Physiol.

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“…These extracts were evaporated at reduced pressure, taken up in petroleum ether, and separated into four fractions (cholesterol esters, triglycerides, free fatty acids, and phospholipids) by silicic acid chromatography (Lis, Tinoco, and Okey 1961). For ease in estimation, columns were run in duplicate-fractions collected from one column were methylated for separation by gas chromatography while those from the second column were assayed for lipid content by gravimetric means.…”

Section: (B) Extraction and Fractionation Of Lipidsmentioning

confidence: 99%

Fatty Acid Components of Ovine Tissue Lipids, and The Response to Prolonged Protein Depletion

Horgan¹,

Masters²

1963

Aust. Jnl. Of Bio. Sci.

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SummaryThe lipids of ovina liver and plasma have been separated by silicic acid chromatography into cholesterol ester, triglyceride, free fatty acid, and phospholipid fractions. Fatty acid constituents of these fractions have been determined by gas chromatography. The lipid content of these tissues was also determined for animals subjected to a prolonged protein depletion. Several significant differences were caused by the experimental diet, with the liver showing more alterations than plasma. The unsaturated C 18 fatty acids were affected to the greatest degree.Implications of these results have been discussed in relation to comparative biochemistry and intermediary lipid metabolism.

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“…3 porosity). The purified lipids were fractionated by the micro-method of Lis, Tinoco & Okey (1961). The Jipid mixture (3-7mg.)…”

Section: Methodsmentioning

confidence: 99%

The extraction of cell walls of Pseudomonas aeruginosa with aqueous phenol. Material from the phenol layer

Clarke

Gray

Reaveley

1967

Biochemical Journal

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1. A method is described for the fractionation of cell walls of Pseudomonas aeruginosa by using aqueous phenol. 2. With this technique, cell walls are quantitatively separated into five fractions: two water-soluble fractions (AqI and AqII), a residual insoluble fraction (R), and two phenol-soluble fractions (PhMP and PhMS). 3. The compositions of fractions PhMP and PhMS are discussed. Fraction PhMP consists almost entirely of protein, which is soluble in aqueous sodium dodecyl sulphate. Analytical ultracentrifugation of the solution yields a single peak, but several components may be resolved by gel electrophoresis. 4. Fraction PhMS consists principally of phospholipid (approx. 44%) and fatty acids (approx. 27%), although smaller amounts (approx. 13%) of protein are present.

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A micromethod for fractionation of lipids by silicic acid chromatography

Cited by 77 publications

References 6 publications

Effect of Growth Temperature on the Lipid Composition of Cyanidium caldarium

Effect of Growth Temperature on the Lipid Composition of Cyanidium caldarium

Fatty Acid Components of Ovine Tissue Lipids, and The Response to Prolonged Protein Depletion

The extraction of cell walls of Pseudomonas aeruginosa with aqueous phenol. Material from the phenol layer

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