A micromethod for the quantitation of sialoglycoconjugate immobilization on insoluble supports: Its use in the preparation of supports with varying ligand concentration

Corfield, Anthony P.; Parker, Terry L.; Schauer, Roland

doi:10.1016/0003-2697(79)90223-9

Cited by 9 publications

(2 citation statements)

References 22 publications

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“…This ensures that the xyloglucan is covalently bound to the AFM tip at one end only. The unstable Schiff's base is then reduced by sodium cyanoborohydride to give a stable secondary amine [8,9].…”

Section: Covalent Binding Of Xyloglucan Molecules To the Afm Tipmentioning

confidence: 99%

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The self-assembly of plant cell wall components by single-molecule force spectroscopy and Monte Carlo modelling

Sa¹,

Hanna²,

Miles³

2004

Nanotechnology

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The nature of the interaction between plant cell wall polysaccharides was investigated by force spectroscopy and computer simulations. Single xyloglucan molecules were tethered between a cellulose substrate and an atomic force microscope tip, which was cycled at rates from 0.1 to 7 Hz. The force–extension curves showed force plateaux of tens of piconewtons, in some cases multiple, with no clear rate dependence. Similar force plateaux were predicted by simulations of polymer chains adsorbed to a flat surface and a virtual cantilever, using a coarse-grained Monte Carlo approach. The simulated plateaux were five to ten times smaller than those obtained by experiment. The experimental plateau energies suggest that at least one hydrogen bond per backbone glucose residue is involved in the interaction. Multiple plateaux always decrease in height with extension, implying a self-tensioning system. This behaviour in a biological nanocomposite could have applications in the manufacture of novel synthetic nanocomposites.

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Section: Covalent Binding Of Xyloglucan Molecules To the Afm Tipmentioning

confidence: 99%

“…Computer simulations were also performed in order to inform the interpretation of the experimental data. Because of the desirability of tethering molecules to the AFM tip covalently at one end of the molecule [7], a novel method was developed for functionalizing the tip, which made use of a Schiff's base intermediate [8,9].…”

Section: Introductionmentioning

confidence: 99%