2022
DOI: 10.1002/pat.5753
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A microplate‐based Response Surface Methodology model for growth optimization and biofilm formation on polystyrene polymeric material in a Candida albicans and Escherichia coli co‐culture

Abstract: Biofilm formation is one of the crucial factors for virulence in Candida albicans, Escherichia coli and their polymicrobial biofilm formed on medical prosthetics. In this study, high biofilm forming C. albicans M207 from a case of invasive candidiasis and a standard strain of bacterial culture E. coli ATCC 39936 were used for initial screening and media optimization using One Variable at a time (OVAT) and Response Surface Methodology (RSM) models, respectively, in polystyrene microtiter plates for in vitro bio… Show more

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Cited by 20 publications
(5 citation statements)
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“…The application of RSM in optimizing C. albicans growth and biofilm formation offers a promising approach for future research in screening therapeutic interventions and advancing the control of biofilm-related challenges. This novel method significantly enhances our understanding of pathogenic biofilm interactions and paves the way for effective biofilm management strategies [ 34 , 35 ].…”
Section: Discussionmentioning
confidence: 99%
“…The application of RSM in optimizing C. albicans growth and biofilm formation offers a promising approach for future research in screening therapeutic interventions and advancing the control of biofilm-related challenges. This novel method significantly enhances our understanding of pathogenic biofilm interactions and paves the way for effective biofilm management strategies [ 34 , 35 ].…”
Section: Discussionmentioning
confidence: 99%
“…A biofilm of E. coli was prepared on a polystyrene plate by using the protocol developed by Ashrit et al 40 The bacterial viability was investigated via an orange acridine test coupled with fluorescence microscopy observation. 41…”
Section: Methodsmentioning
confidence: 99%
“…using microplates [45,46]. Based on these techniques, a method has been established to measure polymicrobial biofilm formation by C. albicans and E. coli [47]. Moreover, the method can be used to identify samples that inhibit the polymicrobial biofilm formation of C. albicans and E. coli [48].…”
Section: Plos Onementioning
confidence: 99%