A Microplate Format for Characterizing the Growth of Molybdenum-reducing Bacteria

Shukor, Mohd Shukri; Shukor, Mohd Yunus

doi:10.54987/jemat.v2i2.164

Cited by 12 publications

(17 citation statements)

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“…Then the ABIS online system was utilized to interpret the results carried out via [29]. Preparation of resting cells for molybdenum reduction characterization using microplate or microtiter format resting cells as before [30]. To monitor Mo-blue production, the plate was first incubated at room temperature or designated temperatures when the temperature was optimized and then read at 750 nm using a microplate reader with the specific extinction coefficient of 11.69 mM.…”

Section: Isolation Of Molybdenum-reducing Bacteriummentioning

confidence: 99%

Isolation and Characterization of a Molybdenum-reducing and the Congo Red Dye-decolorizing Pseudomonas putida strain Neni-3 in soils from West Sumatera, Indonesia

Rusnam

Gusmanizar

2022

J Biochem Microbiol Biotechnol

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The elimination of heavy metals and organic contaminants, such as phenols, hydrocarbons, and amides, by bioremediation, is the most effective choice for the foreseeable future. This is especially true at low levels, where other methods, such as physical or chemical methods, may not be successful. Each year, a few million tons of these contaminants are emitted. In this study, we examined the ability of a molybdenum-reducing bacteria that were isolated from polluted soil to decolorize azo dyes independently of its ability to reduce molybdenum. The ideal conditions for the bacterium to convert molybdate to molybdate blue are a pH range of 6.0 to 6.5 and a temperature range of 25 to 37 degrees Celsius. After glucose, fructose and galactose were the most effective donors of electrons to enable the reduction of molybdate. Galactose was the least effective supplier of electrons. There are a few other prerequisites that need to be met as well, such as a phosphate concentration of between 2.5 and 7.5 mM and a molybdate concentration of between 10 and 15 mM. Its absorption spectra were identical to that of the phosphomolybdate reduction process and to that of the earlier Mo-reducing bacterium. At a concentration of 2 ppm, the heavy metals Ag (I), Hg (II), and Cu (II) each inhibited the reduction of molybdenum by a per centage of 62.8, 61.1, and 36.8 per cent, respectively. We put the bacterium through a test to see if it can remove the color from a variety of dyes. The Congo Red dye was able to lose its color when exposed to the bacterium. Based on the results of the biochemical study, the bacterium has been provisionally identified as Pseudomonas putida strain Neni-3. This bacteria's ability to detoxify various toxicants is a desirable quality, as it makes the bacterium an efficient bioremediation approach. As a result, this bacterium is in high demand. Purification of the molybdenum-reducing enzyme that was produced by this bacterium is presently being studied in order to characterize decolorization research in a more accurate manner.

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Section: Isolation Of Molybdenum-reducing Bacteriummentioning

confidence: 99%

Isolation and Characterization of a Molybdenum-reducing and the Congo Red Dye-decolorizing Pseudomonas putida strain Neni-3 in soils from West Sumatera, Indonesia

Rusnam

Gusmanizar

2022

J Biochem Microbiol Biotechnol

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“…The effects of pH, phosphate, temperature, and sodium molybdate concentrations on molybdenum reduction to Mo-blue were studied statically employing the resting cells form in a microplate or microtiter format, as had been described earlier [23]. Growth was carried out on an orbital shaker at room temperature and shaken at 120 rpm in a 1-L overnight culture in High Phosphate medium (HPM).…”

Section: Preparation Of Bacterial Resting Cellsmentioning

confidence: 99%

“…strain DRY1 [35] that have been reported previously. In this study the microplate format was employed to expedite characterization work and collect more data than the conventional shake-flask technique [23,40]. Ghani et al initiated the use of resting cells under static circumstances to study bacterial molybdenum reduction [25].…”

Section: Identification Of Molybdenum Reducing Bacteriummentioning

confidence: 99%

Characterization of a Molybdenum-reducing and Phenol-degrading Pseudomonas sp. strain Neni-4 from soils in West Sumatera, Indonesia

Rusnam

Gusmanizar

Rahman

et al. 2022

Bull Environ Sci Sust Manage

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Millions of tonnes of these chemicals are manufactured each year and a large quantity is determined to be contaminating the environment, making them important worldwide pollutants. The fact that they pollute the environment is a major problem on a worldwide scale. There is a continuing search for bioremediation of these contaminants employing bacteria capable of numerous detoxifications. Analysis of the bacterium yielded a preliminary identification of the organism as Pseudomonas aeruginosa Neni-4. Screening for the capacity of molybdenum-reducing bacteria to decolorize different polyphenols was conducted in this study. Reduction was optimum at pH 6.3 and between 25 and 40 oC. The bacterium used glucose as the best carbon source or molybdenum reduction followed by galactose, 2-ketogluconate, and citrate in decreasing order. Phosphate between 5.0 and 7.5 mM and sodium molybdate between 15 and 20 mM maximally supported reduction. Like earlier Mo-reducing bacteria, a reduction of phosphomolybdate is seen in the absorption spectra of the Mo-blue generated. Heavy metals prevented molybdenum reduction. None of the phenolic compounds can reduce molybdenum when provided as sole carbon sources. In contrast, the bacterium was able to grow on phenol, benzoate, salicylic acid, and catechol, all of which are substances that include phenolic components. A significant bioremediation technology is this bacterium's capacity to metabolise molybdenum and thrive on poisonous phenolics.

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“…As previously developed, monitoring of Mo-blue output at different sodium molybdate concentrations was carried out statically using resting cells in a microplate or microtiter format. [46]. Cells was grown overnight in High Phosphate media (HPM) at room temperature on orbital shaker (150 rpm) with the phosphate concentration fixed at 100 mM.…”

Section: Preparation Of Resting Cells For Molybdenum Reduction Charac...mentioning

confidence: 99%

Mathematical Modeling of the Molybdenum Blue Production from Serratia sp. strain DRY5

Syed

Shamaan

Shukor

2020

J Environ Microbiol Toxicol

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The reduction of molybdenum to molybdenum blue is a detoxification process and the development of Mo-blue is associated with growth. Significant parameters such as precise reduction rate, theoretical maximum reduction and whether reduction at high molybdenum concentration influenced the lag time of reduction can be discovered by mathematical modeling of the reduction phase. While common, the use of the linearization method by the use of natural logarithm transformation is inaccurate and can only provide an approximate value for the calculated single parameter, the real growth rate. In this work, a variety of models for such as logistic, Gompertz, Richards, Schnute, Baranyi-Roberts, Von Bertalanffy, Buchanan three-phase and more recently Huang were utilized to obtain values for the above parameters or constants. The Huang model was the best model in modelling the Mo-blue production curve of the Serratia sp. strain DRY5 based on statistical tests such as root-mean-square error (RMSE) (0.043), adjusted coefficient of determination (R2) (0.994), bias factor (BF) (1.00), accuracy factor (AF) (1.03) and corrected AICc (Akaike Information Criterion) (-67.02). Parameters obtained from the fitting exercise were maximum Mo-blue production rate (ïm), lag time (ï¬) and maximal Mo-blue production (Ymax). We make use of primary growth models for modeling Mo-blue output in this work. This is an emerging method of identifying constants of parameters that control the reduction of molybdenum. For the creation of further secondary models, the constants calculated from this work will be utilized for such purpose. The use of these primary models can also be broadened to processes involving the detoxification of other heavy metals.

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A Microplate Format for Characterizing the Growth of Molybdenum-reducing Bacteria

Cited by 12 publications

References 0 publications

Isolation and Characterization of a Molybdenum-reducing and the Congo Red Dye-decolorizing Pseudomonas putida strain Neni-3 in soils from West Sumatera, Indonesia

Isolation and Characterization of a Molybdenum-reducing and the Congo Red Dye-decolorizing Pseudomonas putida strain Neni-3 in soils from West Sumatera, Indonesia

Characterization of a Molybdenum-reducing and Phenol-degrading Pseudomonas sp. strain Neni-4 from soils in West Sumatera, Indonesia

Mathematical Modeling of the Molybdenum Blue Production from Serratia sp. strain DRY5

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