A microplate incubation method for assessing egg quality of the barfin flounder: effects of well size and rearing medium on larval viability

Unuma, Tatsuya; Ichikawa, Takashi; Sawaguchi, Sayumi; Hasegawa, Natsuki

doi:10.1007/s10499-018-0316-5

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…The survival rate of the eggs was calculated as follows: survival rate (%) = (number of viable eggs/number of total eggs) Â 100. To determine the normal hatching rate (hereafter referred to as hatching rate) of the eggs subjected to simulated transportation, approximately 100 floating eggs were collected and incubated in the wells of six-well plates (30-33 eggs/well) filled with UV-sterilized seawater (5 mL/well) containing antibiotics (50 IU/mL penicillin and 50 μg/mL streptomycin, Nacalai Tesque, Kyoto, Japan) at 24 C in an incubator until hatching (Hayashida et al, 2021;Higuchi et al, 2015;Unuma et al, 2004Unuma et al, , 2019. The number of normally hatched larvae (with straight bodies) (Higuchi et al, 2015) was counted under a light microscope (SZX-7).…”

Section: Experiments 1: Simulated Egg Transportationmentioning

confidence: 99%

Optimization of stocking density and shipping duration for transportation of Pacific bluefin tuna, Thunnus orientalis (Temminck et Schlegel), eggs, and experimental verification of polyethylene glycol treatment to reduce collision damage

Hayashida

Higuchi

Hashimoto

et al. 2023

J World Aquaculture Soc

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Pacific bluefin tuna (PBT), Thunnus orientalis, is one of the most important species for aquaculture worldwide. For the conservation of this species and sustainable development of the tuna farming industry, production based on closed‐cycle aquaculture should be promoted. In this study, to develop an efficient method for transporting PBT eggs, we simulated egg transportation to optimize stocking density and shipping duration, which are the two variables critical to the transportation procedure of PBT. Based on the findings of this study, we concluded that PBT eggs should be transported at a density of 1 × 104 eggs/L within 12 h in the field. Furthermore, we showed that collisions between eggs during transportation negatively affected the normal hatching rates of eggs, and polyethylene glycol (PEG) treatment (at 1000 μg/mL for 12 h) could reduce such damage. Our results suggest that PEG treatment is a practical approach that contributes to the stable transportation of PBT eggs, particularly in cases where the effects of physical shock damage is considerably increased.

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Section: Experiments 1: Simulated Egg Transportationmentioning

confidence: 99%