A Microsatellite-Based Multipoint Index Map of Human Chromosome 22

No evidence for linkage of chromosome 22 markers to schizophrenia in Southern African Bantu-speaking families

“…Nine previously described polymorphic loci were chosen in the region 22qll-q13, i.e., eight highly polymorphic short tandem repeats (STRs) [Buetow et al, 1993;Gyapay et al, 19941 and one restriction fragment length polymorphism (RFLP) [Tenhunen et al, 19941. Four of the STRs have heterozygosities >0.8, three >0.6, and one >0.5.…”

The t(II; 22)(pI5.5; qII.23) in a retroperitoneal rhabdoid tumor also includes a regional deletion distal to CRYBB2 on 22q

“…Since the first cytogenetic observation of a 22qll deletion in a malignant rhabdoid of the brain (Douglas et al, 1990), it has become increasingly evident that the q l l band of chromosome 22 is involved in the development of MRTs arising at different anatomic sites. In this study, we have further mapped the t(11;22)(p15.5;qll.3) identified in the retroperitoneal M R T cell line TM87-16 (Kames et al, 1991) and have shown that the translocation breakpoint is located between the CRYBBZ and DZ2SZ58 markers, which are separated by an estimated 1.1 cM (Buetow et al, 1993). T h e translocation occurred about 2 Mb distal to BCR and was accompanied by a deletion of a least 2 Mb in the vicinity of CRYBBZ, which includes the D22S258, CRYBA4, D22S300, D22S1, and D22S310 loci and, potentially, CRYBBZPZ.…”

“…T h e 14 primer sets utilized were designed from the published sequences: D22S303, TOPIPZ, D22S343, CRYBBZ, D22S258, D22S300, D22S310, and ILZRB (Buetow et al, 1993); CRYBBZPI and D22S1 (Collins et al, 1992); D22S42, D22S32, and D22S29 (MacCollin et al, 1993); and CRYBA4 (Bijlsma et al, 1993). T h e primer end-labeling procedure included 16-100 pM primer, 30 pCi [y3'P]dATP (Amersham), and 30 units T 4 polynucleotide kinase (NEB) incubated in a 25 p1 reaction at 37°C for 2 h, followed by 65°C for 10 min.…”

Cytogenetic and molecular analysis of a t(1;22)(p36;q11.2) in a rhabdoid tumor with a putative homozygous deletion of chromosome 22

“…This region contains, as the most centromeric marker, D22S257, which is located in BCR intron 1. D22S301, which maps 1.4 cM telomeric to D22S257, is immediately distal to the overlapping region of the 361D9 and 446B5 YAC clones (Buetow et al, 1993).…”