A microsurgical method for experimental kidney denervation

Kullendorff, Carl‐Magnus

doi:10.1002/micr.1920070209

Microsurgery

1986

DOI: 10.1002/micr.1920070209

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A microsurgical method for experimental kidney denervation

Carl‐Magnus Kullendorff

Abstract: A successful microsurgical method for denervation of the kidney in the rat is presented. Unilateral kidney denervation was performed in eight animals. Ten sham-operated animals were used as controls. Under ether anaesthesia, the kidney vessels on one side were exposed. The peritoneum above the vessels was incised and the area was stained with 1% toluidine blue solution. The nerves were identified. With microsurgical technique, the nerves were carefully separated from the vessels and resected. Tissue specimens … Show more

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Cited by 4 publications

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Heterotopic renal transplantation in the rat: An advanced microsurgical training exercise

Korber

Kraemer

1988

Microsurgery

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Microsurgical training programs have instructed trainees from many surgical specialties with the expansion of these techniques into all areas of clinical surgery and experimental research. Most programs employ the rat femoral vessel model in microsurgical training. A more complex and less frequently used model, the rat renal transplantation procedure, can be used for advanced training in microsurgical and microurological techniques and offers several advantages over other microsurgical models. In the heterotopic renal transplant model, the left kidney from a donor rat is transferred to the inguinofemoral region of a syngeneic recipient. The method of revascularization can be varied from end-to-end repairs of vessels of equal diameter to those of unequal diameter. In addition to the variety of possible vascular repairs, an advantage of this model over others is that the kidney graft can be harvested by a technician, thus reducing the time commitments of the trainee. This model is ideal for microsurgeons, immunologists, and urologists, since it affords the opportunity for developing technical skills while preparing an experimental model that can be used for further research. We describe the model, the operative techniques, and the variations one can employ in performing the vascular repairs.

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Heterotopic renal transplantation in the rat: An advanced microsurgical training exercise

Korber

Kraemer

1988

Microsurgery

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Influence of renal denervation on vascular responsiveness of isolated rat intrarenal arteries

Högestätt

Johansson

Andersson

et al. 1988

Acta Physiologica Scandinavica

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Microsurgical renal denervation of the rat has been reported to increase blood loss and bleeding time after a standardized kidney resection. To investigate the vascular effects of denervation, isolated intrarenal arteries were studied using sensitive 'isometric' recording equipment. Four pieces of evidence were obtained to indicate an effective functional denervation I week after renal nerve transection: (i) Phentolamine reduced the K+-induced contraction in controls but not in denervated arteries. (ii) The K+-induced contraction was significantly smaller in denervated than in control arteries. (iii) Noradrenaline (NA) was a significantly more potent vasoconstrictor (4 x) in denervated than in control arteries. (iv) Cocaine increased the NA sensitivity in control arteries (3 x), whereas it failed to do so in denervated vessels. Vasopressin, 5-hydroxytryptamine (5-HT), NA (in the presence of cocaine), prostaglandin F2 alpha (PGF2 alpha) and dopamine (DA) produced concentration-dependent contractions in the mentioned order of potency. Denervated arteries were found to be about two to three times more sensitive to the vasoconstrictors than control arteries. Angiotensin I and II had no contractile effect in any of the vessel segments examined. Indomethacin-pretreated arteries also failed to respond to angiotensin II. Neuropeptide Y produced only weak contractions and failed to influence the NA concentration-response relationship in either control or denervated arteries. In conclusion, renal denervation caused a general supersensitivity of the vascular smooth muscle cells to both circulating and non-circulating vasoconstrictors. Our results cannot explain the increased blood loss and bleeding time seen after denervation, but rather support the view that the enhanced bleeding was caused by an interrupted vasoconstrictor influence of the sympathetic nerves.

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Influence of renal sympathectomy, sodium depletion and prostaglandin synthetase inhibition on prostaglandin production and [³H]PGE₂ binding characteristics in rat kidney

Eriksson

Larsson

Kullendorff

et al. 1990

Acta Physiologica Scandinavica

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The effects of renal sympathectomy (unilateral, renal microsurgical denervation), sodium depletion (hypovolaemia) and prostaglandin synthetase inhibition on the rate of prostaglandin synthesis and [3H]PGE2 binding characteristics were studied in the rat kidney. The intrarenal rate of prostaglandin synthesis was measured by monitoring the urinary excretion of 6-keto-PGF1 alpha, the stable hydration product of prostacyclin. Dietary sodium restriction was associated with a 99% decrease in urinary sodium excretion (P less than 0.001) and a 17% decrease of urine volume (n.s.). Renal denervation or sodium deprivation changed neither the rate of excretion of 6-keto-PGF1 alpha nor the density or affinity of [3H]PGE2 binding sites as compared to control. However, in sodium-depleted rats, prostaglandin synthesis inhibition, induced by naproxen, decreased the urinary excretion of 6-keto-PGF1 alpha by 40% (P = 0.011) and increased the number of [3H]PGE2 binding sites by almost 30% (P = 0.031) with no change in binding affinities as compared with sodium-depleted controls. In contrast, sulindac was not able to suppress the renal synthesis and excretion of 6-keto-PGF1 alpha, and did not modulate the [3H]PGE2 binding characteristics. The lack of effect on the excretion of 6-keto-PGF1 alpha and on the [3H]PGE2 binding characteristics supports the view that sulindac spares renal prostaglandin synthesis.

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A microsurgical method for experimental kidney denervation

Cited by 4 publications

References 6 publications

Heterotopic renal transplantation in the rat: An advanced microsurgical training exercise

Heterotopic renal transplantation in the rat: An advanced microsurgical training exercise

Influence of renal denervation on vascular responsiveness of isolated rat intrarenal arteries

Influence of renal sympathectomy, sodium depletion and prostaglandin synthetase inhibition on prostaglandin production and [³H]PGE₂ binding characteristics in rat kidney

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A microsurgical method for experimental kidney denervation

Cited by 4 publications

References 6 publications

Heterotopic renal transplantation in the rat: An advanced microsurgical training exercise

Heterotopic renal transplantation in the rat: An advanced microsurgical training exercise

Influence of renal denervation on vascular responsiveness of isolated rat intrarenal arteries

Influence of renal sympathectomy, sodium depletion and prostaglandin synthetase inhibition on prostaglandin production and [3H]PGE2 binding characteristics in rat kidney

Contact Info

Product

Resources

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Influence of renal sympathectomy, sodium depletion and prostaglandin synthetase inhibition on prostaglandin production and [³H]PGE₂ binding characteristics in rat kidney