A microtest system for the serial assay of phytotoxic compounds using photoautotrophic cell suspension cultures of Chenopodium rubrum

Abstract

Chenopodium rubrum photoautotrophic cell suspensions were grown in plastic tissue culture dishes under photoautotrophic conditions. Growth was monitored by measuring cell number, packed cell volume, chlorophyll content and oxygen production. Such microtiter dishes are suitable systems for the serial assay of growth inhibition and various physiological effects (i.e. chlorophyll fluorescence, cell viability, oxygen production) of photoautotrophic cells as caused by herbicides and fungal phytotoxins. The applicability of the test system is discussed.

Metribuzin resistance in photoautotrophic Chenopodium rubrum cell cultures

“…The determination of the PI,, (-log I,,) values and R/S (I,, resistant/I,, wild-type) values involved estimation of the concentration required for 50% inhibition (I,,) [16].…”

Noninvasive determination of toxic stress biomarkers by high-throughput screening of photoautotrophic cell suspension cultures with multicolor fluorescence imaging

“…Cell suspension cultures are grown in axenic conditions, the cells are exposed to all treatments directly and homogeneously, which results in fast and homogeneous signal readouts. Cell suspension cultures are suitable for experiments in microtiter plates as indicated by herbicide testing [11–13], allowing for high-throughput data acquisition. Besides the application of cell suspension cultures in biotechnology [14–18] and basic research of various processes at the cellular level [19–23], successful studies of herbicides [12, 13, 24], toxicity screenings [4], salt tolerance [25] and deriving crop lines resistant to various toxicant types [26, 27] were also conducted.…”

“…Among cell suspension cultures, it is PA cell suspension cultures that are especially valuable. The presence of functional chloroplasts enables testing of substances that directly affect photosynthesis and the use of methods based on photosynthesis monitoring [13, 17, 23, 28]. PA cell suspension cultures are available for various plant species including crop, medicinal and model species [29].…”