A Microtiter Plate Procedure for Evaluating Fungal Functional Diversity

Dobranic, Jason K.; Zak, John C.

doi:10.2307/3761529

Cited by 84 publications

(44 citation statements)

References 25 publications

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“…A tetrazolium dye sensitive to reduction is included with each C substrate on bacterial microtiter plates. Fungal microtiter plates do not include the tetrazolium dye due to toxicity to some fungi [61]. This dye develops a purple color if catabolized, while fungal microtiter plates utilize turbidity as a measurement.…”

Section: Microbial Activity and Net N Transformationsmentioning

confidence: 99%

Short-Term Belowground Responses to Thinning and Burning Treatments in Southwestern Ponderosa Pine Forests of the USA

Overby

Hart

2016

Forests

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Microbial-mediated decomposition and nutrient mineralization are major drivers of forest productivity. As landscape-scale fuel reduction treatments are being implemented throughout the fire-prone western United States of America, it is important to evaluate operationally how these wildfire mitigation treatments alter belowground processes. We quantified these important belowground components before and after management-applied fuel treatments of thinning alone, thinning combined with prescribed fire, and prescribed fire in ponderosa pine (Pinus ponderosa) stands at the Southwest Plateau, Fire and Fire Surrogate site, Arizona. Fuel treatments did not alter pH, total carbon and nitrogen (N) concentrations, or base cations of the forest floor (O horizon) or mineral soil (0-5 cm) during this 2-year study. In situ rates of net N mineralization and nitrification in the surface mineral soil (0-15 cm) increased 6 months after thinning with prescribed fire treatments; thinning only resulted in net N immobilization. The rates returned to pre-treatment levels after one year. Based on phospholipid fatty acid composition, microbial communities in treated areas were similar to untreated areas (control) in the surface organic horizon and mineral soil (0-5 cm) after treatments. Soil potential enzyme activities were not significantly altered by any of the three fuel treatments. Our results suggest that a variety of one-time alternative fuel treatments can reduce fire hazard without degrading soil fertility.

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Section: Microbial Activity and Net N Transformationsmentioning

confidence: 99%

Short-Term Belowground Responses to Thinning and Burning Treatments in Southwestern Ponderosa Pine Forests of the USA

Overby

Hart

2016

Forests

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“…where P i (in the BIOLOG experiment) is a probability that represents the difference of OD value between a medium pore and control, namely P i ¼ ðC−RÞ=∑ðC−RÞ (Dobranic and Zak, 1999). The natural logarithm was used in this paper where P i (in the DGGE experiment) stands for the observed number of clones of a given species divided by the total number of organisms.…”

Section: Statistical Analysis Of Biolog and Dggementioning

confidence: 99%

Structural and metabolic responses of microbial community to sewage-borne chlorpyrifos in constructed wetlands

Zhang

Wang

Zhang

et al. 2016

Journal of Environmental Sciences

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Long-term use of chlorpyrifos poses a potential threat to the environment that cannot be ignored, yet little is known about the succession of substrate microbial communities in constructed wetlands (CWs) under chlorpyrifos stress. Six pilot-scale CW systems receiving artificial wastewater containing 1 mg/L chlorpyrifos were established to investigate the effects of chlorpyrifos and wetland vegetation on the microbial metabolism pattern of carbon sources and community structure, using BIOLOG and denaturing gradient gel electrophoresis (DGGE) approaches. Based on our samples, BIOLOG showed that Shannon diversity (H V ) and richness (S) values distinctly increased after 30 days when chlorpyrifos was added. At the same time, differences between the vegetated and the non-vegetated systems disappeared. DGGE profiles indicated that H Vand S had no significant differences among four different treatments. The effect of chlorpyrifos on the microbial community was mainly reflected at the physiological level. Principal component analysis (PCA) of both BIOLOG and DGGE showed that added chlorpyrifos made a difference on test results.Meanwhile, there was no difference between the vegetation and no-vegetation treatments after addition of chlorpyrifos at the physiological level. Moreover, the vegetation had no significant effect on the microbial community at the genetic level. Comparisons were made between bacteria in this experiment and other known chlorpyrifos-degrading bacteria. The potential chlorpyrifos-degrading ability of bacteria in situ may be considerable.

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“…The substrates were classified into six substrate guilds, namely amines, amino acids, carbohydrates, carboxylic acids, polymers and miscellaneous, according to Dobranic and Zak (1999). Prior to the Biolog Ò analysis, the field-moist soil samples were wetted up to 50 % of their maximal WHC and pre-incubated during 1 week at 22°C.…”

Section: Biologmentioning

confidence: 99%

“…The absorbance values for individual wells/substrates were expressed as a proportion of the total sample absorbance on the plate, standardised to 1 for each sample. In addition, the mean absorbance for each site was divided into six substrate guilds: amines, amino acids, carbohydrates, carboxylic acids, polymers and miscellaneous, according to Dobranic and Zak (1999).…”

Section: Biologmentioning

confidence: 99%

The relationship between soil bacteria substrate utilisation patterns and the vegetation structure in temperate forests

et al. 2015

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The aim of the study was to explore the relationship between the functional diversity pattern of soil bacteria and the vegetation diversity and structure in temperate forests (Poland). Pine-dominated forests occur on soils with lower pH, fewer nutrient contents (P, Na, Mg, Mn and K) and higher C/N and C/P ratios than beechdominated forests and mixed broadleaved forest with hornbeam and ash. Both forest type and soil horizon (O and A) strongly influenced bacterial catabolic activity and the number of substrates decayed on Biolog Ò ECO plates. Pine forest soil bacteria were less active and less functionally diverse than those in deciduous forest soils. The community-level physiological profiles (CLPPs) were dissimilar (one-way analysis of similarities) between pine and mixed deciduous forests, but only in the O soil horizon. Carboxylic acids primarily contributed to the average dissimilarity in CLPP between forests (the similarity percentage procedure); these substrates are preferentially used by pine forest soil bacteria. The canonical correspondence analysis indicated that soil pH, nitrogen and organic matter contents and plant diversity index H 0 plant were related to bacterial CLPP in the O soil horizon. Only for the soil O horizon, the Mantel test showed a clear relationship between vegetation structure and bacterial CLPP.

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A Microtiter Plate Procedure for Evaluating Fungal Functional Diversity

Cited by 84 publications

References 25 publications

Short-Term Belowground Responses to Thinning and Burning Treatments in Southwestern Ponderosa Pine Forests of the USA

Short-Term Belowground Responses to Thinning and Burning Treatments in Southwestern Ponderosa Pine Forests of the USA

Structural and metabolic responses of microbial community to sewage-borne chlorpyrifos in constructed wetlands

The relationship between soil bacteria substrate utilisation patterns and the vegetation structure in temperate forests

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