A microtiter-plate screening method for biofilm disinfection and removal

Pitts, Betsey; Hamilton, Martin A.; Zelver, Nick; Stewart, Philip S.

doi:10.1016/s0167-7012(03)00034-4

Cited by 291 publications

(210 citation statements)

References 23 publications

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“…The development of low cost, high throughput biofilm screening methods in microtitre-plates, have made it possible to identify genes essential for surface-attached biomass production in liquid media [50,51]. This system has been heavily used to identify potential anti-biofilm agents, by measuring the reduction in surface attached biomass on the sides of wells after treatment with potential therapeutic agents [51][52][53][54].…”

Section: In Vitro Investigation Of Biofilmsmentioning

confidence: 99%

“…This system has been heavily used to identify potential anti-biofilm agents, by measuring the reduction in surface attached biomass on the sides of wells after treatment with potential therapeutic agents [51][52][53][54]. In addition to this, The Center for Disease Control (CDC) approved biofilm reactor [55][56][57], and drip flow reactors, have proved excellent for assessing biofilm formation on biological and non-biological materials [58][59][60].…”

Section: In Vitro Investigation Of Biofilmsmentioning

confidence: 99%

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The Limitations of In Vitro Experimentation in Understanding Biofilms and Chronic Infection

Roberts

Kragh

Bjarnsholt

et al. 2015

Journal of Molecular Biology

173

153

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We have become increasingly aware that during infection, pathogenic bacteria often grow in multicellular biofilms which are often highly resistant to antibacterial strategies. In order to understand how biofilms form and contribute to infection, in vitro biofilm systems such as microtitre plate assays and flow cells, have been heavily used by many research groups around the world. Whilst these methods have greatly increased our understanding of the biology of biofilms, it is becoming increasingly apparent that many of our in vitro methods do not accurately represent in vivo conditions. Here we present a systematic review of the most widely used in vitro biofilm systems, and we discuss why they are not always representative of the in vivo biofilms found in chronic infections. We present examples of methods that will help us to bridge the gap between in vitro and in vivo biofilm work, so that our bench-side data can ultimately be used to improve bedside treatment.

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Section: In Vitro Investigation Of Biofilmsmentioning

confidence: 99%

Section: In Vitro Investigation Of Biofilmsmentioning

confidence: 99%

The Limitations of In Vitro Experimentation in Understanding Biofilms and Chronic Infection

Roberts

Kragh

Bjarnsholt

et al. 2015

Journal of Molecular Biology

173

153

View full text Add to dashboard Cite

show abstract

“…Bioluminescence [31] and a microtiter plate test [32] may also be used. Biofilm enumeration significantly depends on the technique used to remove and detach the bacterial cells from the substrate (e.g.…”

Section: Disinfection Efficacy Of Hssp Against E Coli Biofilm Estimmentioning

confidence: 99%

Disinfection Treatment of Heated Scallop-Shell Powder on Biofilm of <i>Escherichia coli</i> ATCC 25922 Surrogated for <i>E. coli</i> O157:H7

Kubo¹,

Ohshima²,

Irie³

et al. 2013

JBNB

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The ability of heated scallop-shell powder (HSSP) to disinfect Escherichia coli ATCC 25922 biofilm was investigated. On account of its cryotolerance and cell surface characteristics, the E. coli strain is reportedly a useful surrogate for E. coli O157: H7 in surface attachment studies. In this study, an E. coli ATCC 25922 biofilm was formed on a glass plate, and immersed in a slurry of HSSP. Following treatment, the disinfection ability of the HSSP toward the biofilm was non-destructively and quantitatively measured by conductimetric assay. The disinfection efficacy increased with HSSP concentration and treatment time. HSSP treatment (10 mg/mL, pH 12.5) for 20 min completely eliminated biofilm bioactivity (approximately 10 8 CFU/cm 2 in non-treated biofilms). In contrast, treatment with NaOH solution at the same pH, and treatment with sodium hypochlorite (200 g/mL) reduced the activity by approximately one to three log 10 . Fluorescence microscopy confirmed that no viable cells remained on the plate following HSSP treatment (10 mg/mL). Although alkaline and sodium hypochlorite treatments removed cells from the biofilm, under these treatments, many viable cells remained on the plate. To elucidate the mechanism of HSSP activity against E. coli ATCC 25922, the active oxygen generated from the HSSP slurry was examined by chemiluminescence analysis. The luminescence intensity increased with increasing concentration of HSSP slurry. The results suggested that, besides being alkaline, HSSP generates active oxygen species with sporicidal activity. Thus, HSSP treatment could also be effective for controlling biofilms of the toxic strain E. coli O157: H7, implicated in food poisoning.

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“…Bioluminescence (Dhir and Dodd, 1995) and a microtiter plate test (Pitts et al, 2003) can also be used. Biofilm enumeration de- biofilms until a technique for removing attached bacteria from substrates is established.…”

Section: Resultsmentioning

confidence: 99%

Ability of Heated Scallop-Shell Powder to Disinfect Staphylococcus aureus Biofilm

Sawai

Ko-jiro

Kikuchi

2013

FSTR

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The ability of heated scallop-shell powder (HSSP) to disinfect Staphylococcus aureus biofilm was investigated. A biofilm of S. aureus NBRC 13276 was produced on a glass plate. Scallop-shell powder was exposed to heat treatment at 1000℃ in air for 1 h, followed by immersion of the biofilm plate into the HSSP slurry. After treatment, the disinfection ability of the HSSP toward the biofilm was measured nondestructively and quantitatively using a conductometric assay. Disinfection efficacy against S. aureus biofilm increased with HSSP concentration and treatment time. HSSP treatment (10 mg/mL, pH 12.5) of 60 min completely eliminated the biofilm bioactivity (which was approximately 10 7 CFU/cm 2 ). In contrast, treatment with NaOH solution (pH 12.5) at a pH identical to the HSSP slurry and treatment with sodium hypochlorite (200 µg/mL) caused an approximately four-to five-order reduction in activity. Fluorescence microscopy confirmed that no viable cells remained on the plate after HSSP treatment. Although alkaline and sodium hypochlorite treatments had a stripping effect on the biofilm, many viable cells remained on the plate. HSSP exerted a disinfection effect against S. aureus biofilm that was greater than that provided by alkaline or sodium hypochlorite treatment. This disinfection effect can be effective for controlling bacteria responsible for food poisoning. The use of HSSP in food processing is expected to not only be a source of minerals but also prolong the shelf-life of foodstuffs and reduce the pollution resulting from discarded shells.

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A microtiter-plate screening method for biofilm disinfection and removal

Cited by 291 publications

References 23 publications

The Limitations of In Vitro Experimentation in Understanding Biofilms and Chronic Infection

The Limitations of In Vitro Experimentation in Understanding Biofilms and Chronic Infection

Disinfection Treatment of Heated Scallop-Shell Powder on Biofilm of <i>Escherichia coli</i> ATCC 25922 Surrogated for <i>E. coli</i> O157:H7

Ability of Heated Scallop-Shell Powder to Disinfect Staphylococcus aureus Biofilm

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A microtiter-plate screening method for biofilm disinfection and removal

Cited by 291 publications

References 23 publications

The Limitations of In Vitro Experimentation in Understanding Biofilms and Chronic Infection

The Limitations of In Vitro Experimentation in Understanding Biofilms and Chronic Infection

Disinfection Treatment of Heated Scallop-Shell Powder on Biofilm of &lt;i&gt;Escherichia coli&lt;/i&gt; ATCC 25922 Surrogated for &lt;i&gt;E. coli&lt;/i&gt; O157:H7

Ability of Heated Scallop-Shell Powder to Disinfect Staphylococcus aureus Biofilm

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Disinfection Treatment of Heated Scallop-Shell Powder on Biofilm of <i>Escherichia coli</i> ATCC 25922 Surrogated for <i>E. coli</i> O157:H7