In this paper, a real-world transportation problem is addressed, concerning the collection and the transportation of biological sample tubes from sampling points to a main hospital. Blood and other biological samples are collected in different centers during morning hours. Then, the samples are transported to the main hospital, for their analysis, by a fleet of vehicles located in geographically distributed depots. Each sample has a limited lifetime and must arrive to the main hospital within that time. If a sample cannot arrive to the hospital within the lifetime, either is discarded or must be processed in dedicated facilities called Spoke Centers.