A miniaturized electrothermal array for rapid analysis of temperature preference behaviors in ecology and ecotoxicology

Henry, Jason; Bai, Yutao; Kreuder, Florian; Saaristo, Minna; Kaslin, Jan; Wlodkowic, Donald

doi:10.1016/j.envpol.2022.120202

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…We could not find any study that exposed planarians to another NSAID in a thermotaxis essay. Henry et al set up a protocol highlighting planarian thermotaxis and showed that ibuprofen, another NSAID, managed to modulate thermotaxis on zebrafish, but unfortunately did not expose planarians to it ( Henry et al, 2022 ). In our study, not only did we show that meloxicam could reduce noxious reactions induced by an irritant, but that it also managed to modulate planarian heat avoidance, making them prone to spend more time in hotter conditions.…”

Section: Discussionmentioning

confidence: 99%

Behavioral and pharmacological characterization of planarian nociception

Reho,

Menger,

Goumon

et al. 2024

Front. Mol. Neurosci.

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IntroductionPain mostly arises because specialized cells called nociceptors detect harmful or potentially harmful stimuli. In lower animals with less convoluted nervous system, these responses are believed to be purely nociceptive. Amongst invertebrate animal models, planarians are becoming popular in a wide range of pharmacological and behavioral studies beyond the field of regeneration. Recent publications led the way on pain studies by focusing on nociceptive behaviors such as the ‘scrunching’ gait displayed under various noxious stimuli, as opposed to the ‘gliding’ gait planarians usually adopt in normal conditions.MethodsIn this study, we adapted commonly used nociceptive tests to further explore nociception in planarians of the species Girardia dorotocephala. By using behavioral analysis in open fields and place preferences, we managed to set up chemical, thermal and mechanical nociceptive tests. We also adapted RNA interference protocols and explored the effects of knocking down TRPA1 ion channels, one of the main effectors of chemically and thermally-induced nociceptive responses in vertebrates.ResultsConsequently, we demonstrated the reliability of the scrunching gait in this planarian species, which they displayed in a dose-dependent manner when exposed to the irritant AITC. We also showed that suppressing the expression of TRPA1 ion channels completely suppressed the scrunching gait, demonstrating the involvement of TRPA1 nociceptors in this nociceptive reaction. Besides, we also explored the effects of two common analgesics that both displayed strong antinociceptive properties. First, morphine reduced the chemically-induced nociceptive scrunching gaits by more than 20% and shifted the EC50 of the dose–response curve by approximately 10 μM. Secondly, the NSAID meloxicam drastically reduced chemically-induced scrunching by up to 60% and reduced heat avoidance in place preference tests.DiscussionThus, we managed to characterize both behavioral and pharmacological aspects of G. dorotocephala’s nociception, further developing the use of planarians as a replacement model in pain studies and more globally the study of invertebrate nociception.

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Section: Discussionmentioning

confidence: 99%

Behavioral and pharmacological characterization of planarian nociception

Reho,

Menger,

Goumon

et al. 2024

Front. Mol. Neurosci.

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“…T-maze chambers were designed using a CorelDraw X3 (Corel Corporation, Ottawa, Ontario, Canada) computer-assisted design (CAD) package. Vessels were fabricated in a fully biocompatible poly(methyl methacrylate) (PMMA) transparent or dyed thermoplastic using a noncontact 30W infrared laser machining system (Universal Laser Systems, Scottsdale, AZ, USA) as described previously. , Fabricated PMMA layers were thermally bonded at 100 °C in a fan-assisted oven for 90 min to create watertight test chambers.…”

Section: Methodsmentioning

confidence: 99%

“…Behavioral data were acquired using a custom built digital video imaging system consisting of a BlackMagic Micro Studio 4K digital camera (BlackMagic Design, Australia) mounted on a vibration-less photographic column (Polaroid M3, Polaroid Inc., USA). The camera was custom converted to infrared imaging (IR, 850 nm) and paired with a true 1:1 macro-objective lens with a focal length of 30 mm (Olympus, Japan). , Videos were recorded at a resolution of 1920 × 1080 pixels (1080p) and a rate of up to 50 fps. The files were acquired using a PC HDMI interface (BlackMagic 4K Decklink Mini card, BlackMagic Design, Australia) or directly using the recording capabilities of TrackingBot software.…”

Section: Methodsmentioning

confidence: 99%

“…The camera was custom converted to infrared imaging (IR, 850 nm) and paired with a true 1:1 macro-objective lens with a focal length of 30 mm (Olympus, Japan). 28,29 Videos were recorded at a resolution of 1920 × 1080 pixels (1080p) and a rate of up to 50 fps. The files were acquired using a PC HDMI interface (BlackMagic 4K Decklink Mini card, BlackMagic Design, Australia) or directly using the recording capabilities of TrackingBot software.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Toward Real-Time Animal Tracking with Integrated Stimulus Control for Automated Conditioning in Aquatic Eco-Neurotoxicology

Bai,

Henry,

Cheng

et al. 2023

Environ. Sci. Technol.

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Aquatic eco-neurotoxicology is an emerging field that requires new analytical systems to study the effects of pollutants on animal behaviors. This is especially true if we are to gain insights into one of the least studied aspects: the potential perturbations that neurotoxicants can have on cognitive behaviors. The paucity of experimental data is partly caused by a lack of low-cost technologies for the analysis of higher-level neurological functions (e.g., associative learning) in small aquatic organisms. Here, we present a proof-ofconcept prototype that utilizes a new real-time animal tracking software for onthe-fly video analysis and closed-loop, external hardware communications to deliver stimuli based on specific behaviors in aquatic organisms, spanning three animal phyla: chordates (fish, frog), platyhelminthes (flatworm), and arthropods (crustacean). The system's open-source software features an intuitive graphical user interface and advanced adaptive threshold-based image segmentation for precise animal detection. We demonstrate the precision of animal tracking across multiple aquatic species with varying modes of locomotion. The presented technology interfaces easily with low-cost and open-source hardware such as the Arduino microcontroller family for closed-loop stimuli control. The new system has potential future applications in eco-neurotoxicology, where it could enable new opportunities for cognitive research in diverse small aquatic model organisms.

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