This paper presents a frequency-reconfigurable dual-band radio frequency (RF) crossover based on quarter-wavelength coupled lines (CLs) and open stubs. Initially, an even–odd-mode analysis was conducted for the design, and closed-form equations were found. Then an advanced design system (ADS) was utilized to support and further optimize the theoretical analysis. Afterwards, high-frequency simulation software (HFSS) was used to simulate the proposed design. The proposed device is printed on a 1.524 mm RO4003C printed-circuit board (εr=3.55). The frequency tunability is achieved by employing two varactor diodes connected to the open stubs. When the biasing voltage is altered, the capacitance of the SMV1405 varactor can change from 2.67 pF to 0.63 pF. Accordingly, the two operating frequencies can be continuously tuned from 2.06 GHz to 2.40 GHz and from 5.44 GHz to 5.84 GHz. For the low-frequency range, return loss and isolation are above 15 dB, and the insertion loss is less than 1.1 dB. As for the high-frequency range, the return loss is greater than 20 dB, the isolation is better than 15 dB, and the insertion loss is lower than 1.6 dB. The measurement results agreed well with the simulation results, and the crossover overall size is 45.5 mm × 29.4 mm. The proposed device can be utilized for various application areas, such as 5G smartphone applications and satellite communication.