A Minimal Midzone Protein Module Controls Formation and Length of Antiparallel Microtubule Overlaps

Bieling, Peter; Telley, Ivo A.; Surrey, Thomas

doi:10.1016/j.cell.2010.06.033

Cited by 302 publications

(495 citation statements)

References 61 publications

Supporting

Mentioning

452

Contrasting

Order By: Relevance

“…KIF4 (kinesin-4 family) is recruited to the central spindle via its interaction with PRC1, a cross-linker that stabilizes antiparallel microtubule overlaps 159 . In addition to their function in pre-anaphase, KIF4 motors also have a key role in controlling the size of the central spindle through regulation of microtubule dynamics 160,161 KIF4 recruitment to the central spindle is controlled by the phosphorylation of its C-terminal tail by a KIF20A (kinesin-6)-dependent pool of the Aurora B mitotic kinase 162 . KIF2A depolymerase activity is also targeted to distal (minus) ends of central spindle microtubules and drives their shrinkage in order to control the size of the central spindle 163 .…”

Section: Central Spindle Mechanicsmentioning

confidence: 99%

Prime movers: the mechanochemistry of mitotic kinesins

Cross

McAinsh

2014

Nat Rev Mol Cell Biol

188

203

View full text Add to dashboard Cite

Section: Central Spindle Mechanicsmentioning

confidence: 99%

Prime movers: the mechanochemistry of mitotic kinesins

Cross

McAinsh

2014

Nat Rev Mol Cell Biol

188

203

View full text Add to dashboard Cite

“…However, in vivo the length of overlapped MTs is rather minimal in the phragmoplast [6 ]. In vertebrates, the overlap length of PRC1-bundled anti-parallel MTs is controlled via overlap length-dependent microtubule growth inhibition by the Kinesin-4 motor, after it reaches the plus ends via its processive motility that is enhanced by a direct interaction with PRC1 [29 ]. In plants like A. thaliana, Kinesin-4 motors have been assigned to regulate cell elongation in interphase [30].…”

Section: Anti-parallel Mts In the Phragmoplastmentioning

confidence: 99%

The rise and fall of the phragmoplast microtubule array

Lee

Liu

2013

Current Opinion in Plant Biology

View full text Add to dashboard Cite

The cytokinetic apparatus, the phragmoplast, contains a bipolar microtubule (MT) framework that has the MT plus ends concentrated at or near the division site. This anti-parallel MT array provides tracks for the transport of Golgi-derived vesicles toward the plus ends so that materials enclosed are subsequently deposited at the division site. Here we will discuss a proposed model of the centrifugal expansion of the phragmoplast that takes place concomitantly with the assembly of the cell plate, the ultimate product of vesicle fusion. The expansion is a result of continuous MT assembly at the phragmoplast periphery while the MTs toward the center of the phragmoplast are disassembled. These events are the result of MT-dependent MT polymerization, bundling of antiparallel MTs coming from opposite sides of the division plane that occurs selectively at the phragmoplast periphery, positioning of the plus ends of cross-linked MTs at or near the division site by establishing a minimal MT-overlapping zone, and debundling of anti-parallel MTs that is triggered by phosphorylation of MT-associated proteins. The debundled MTs are disassembled at last by factors including the MT severing enzyme katanin. IntroductionThe innovation of the phragmoplast marks a significant advancement in the evolution from green algae to land plants [1]. The phragmoplast contains a core of two mirrored sets of anti-parallel microtubules (MTs) whose plus ends are concentrated at or near the midzone of this cytokinetic apparatus (Figure 1) [2]. The ultimate mission of this bipolar MT array is to allow Golgi-derived vesicles to be transported unidirectionally toward MT plus ends so that the materials enclosed in these vesicles are deposited for the assembly of the cell plate. Phragmoplast MTs, like those MTs in spindles during mitosis, undergo continuous remodeling throughout cytokinesis [3,4]. Upon the completion of anaphase, MTs are polymerized and coalesced in the spindle midzone, and a bipolar array is established as the Kinesin-5 motor acts on anti-parallel MTs to slide them against each other ( Figure 1a) [5,6 ,7]. Concomitant with the addition of more MTs to the array, the MTs are shortened at their minus ends, which are facing the reforming daughter nuclei (Figure 1b). One of the most spectacular phenomena in cytokinesis is the centrifugal expansion of the phragmoplast MT array from the cell center to the edge. During the expansion, new MT filaments are added to the periphery of the phragmoplast while older ones in the inner part of the phragmoplast array are disassembled ( Figure 1c). This is particularly challenging because those opposing activities occur simultaneously within a few microns of each other. The assembly of new MTs uses tubulin subunits released from the depolymerization of older MTs [3].Before MT-associated proteins (MAPs) and MT-based motors were identified, microscopic observations had revealed many structural details of the phragmoplast in elegant systems like the endosperm of the African blood lily Haemanthus and tobacc...

show abstract

“…In vitro experiments at the single molecule level reveal that both PRC1 and Ase1p move diffusively along a single microtubule and have a preference for anti-parallel bundling compared with parallel bundling (Bieling et al, 2010;Kapitein et al, 2008;Subramanian et al, 2010). PRC1 interacts with various motors and MAPs as discussed below.…”

Section: Prc1mentioning

confidence: 99%

“…It moves along the microtubules towards their plus ends and upon its accumulation at the plus end, reduces microtubule polymerisation and depolymerisation dynamics (Bieling et al, 2010;Bringmann et al, 2004). KIF4 was the first kinesin found to associate with mitotic chromosomes and was thus termed chromokinesin (Wang and Adler, 1995).…”

Section: Kif4mentioning

confidence: 99%