A Minireview of the Methods for Listeria monocytogenes Detection

Liu, Aiping; Shen, Lihua; Zeng, Zhenghai; Sun, Min; Liu, Yuntao; Liu, Shuliang; Cheng, Li; Wang, Xiaohong

doi:10.1007/s12161-017-0991-2

Cited by 35 publications

(19 citation statements)

References 98 publications

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“…Member of genus Listeria is a non-spore bacterium, being anaerobic facultative, a small size, Gram-positive, and rod-shaped (0.5-4.0 µm diameter and 0.5-2.0 µm long). Listeria monocytogenes can contaminate a wide range of foods, including yogurt, cheese, meat, ham, smoked salmon, poultry, seafood and vegetable products [2,7].Bacillus cereus is a facultative aerobic to anaerobic, Gram-positive, rod-shaped, and spore-forming bacteria. Spore endurance to unfavorable conditions has assisted the widespread of Bacillus [8,9].…”

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confidence: 99%

“…Spore endurance to unfavorable conditions has assisted the widespread of Bacillus [8,9]. Although the culture method is the gold standard for bacteria identification, it is inefficient, time-consuming (more than 1 week), requires laboratory operator expertise, and identification depends on specific microbiological and biochemical testing [7,9,10]. Besides these methods, there are also other detection methods such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA).…”

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confidence: 99%

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confidence: 99%

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Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus

Astantri

Prakoso

Triyana

et al. 2020

Veterinary Sciences

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The aim of this study is to determine the performance of a lab-made electronic nose (e-nose) composed of an array of metal oxide semiconductor (MOS) gas sensors in the detection and differentiation of Listeria monocytogenes (L. monocytogenes) and Bacillus cereus (B. cereus) incubated in trypticsoy broth (TSB) media. Conventionally, the detection of L. monocytogenes and B. cereus is often performed by enzyme link immunosorbent assay (ELISA) and polymerase chain reaction (PCR). These techniques require trained operators and expert, expensive reagents and specific containment. In this study, three types of samples, namely, TSB media, L. monocytogenes (serotype 4b American Type Culture Collection (ATCC) 13792), and B. cereus (ATCC) 10876, were used for this experiment. Prior to measurement using the e-nose, each bacterium was inoculated in TSB at 1 × 10 3 -10 4 CFU/mL, followed by incubation for 48 h. To evaluate the performance of the e-nose, the measured data were then analyzed with chemometric models, namely linear and quadratic discriminant analysis (LDA and QDA), and support vector machine (SVM). As a result, the e-nose coupled with SVM showeda high accuracy of 98% in discriminating between TSB media and L. monocytogenes, and between TSB media and B. cereus. It could be concluded that the lab-made e-nose is able to detect rapidly the presence of bacteria L. monocytogenes and B. cereus on TSB media. For the future, it could be used to identify the presence of L. monocytogenes or B. cereus contamination in the routine and fast assessment of food products in animal quarantine.Listeria monocytogenes causes the highest case of hospitalization (up to 91%) among other foodborne illnesses [4]. Listeriosis is infectious to humans and mammals, including the ruminant and monogastric animals. The clinical signs of listeriosis in humans include gastroenteritis, diarrhea, meningitis, bacteremia, and it causes encephalitis, septicemia, abortion, mastitis, and gastroenteritis in cows [5,6]. Member of genus Listeria is a non-spore bacterium, being anaerobic facultative, a small size, Gram-positive, and rod-shaped (0.5-4.0 µm diameter and 0.5-2.0 µm long). Listeria monocytogenes can contaminate a wide range of foods, including yogurt, cheese, meat, ham, smoked salmon, poultry, seafood and vegetable products [2,7].Bacillus cereus is a facultative aerobic to anaerobic, Gram-positive, rod-shaped, and spore-forming bacteria. Spore endurance to unfavorable conditions has assisted the widespread of Bacillus [8,9]. Although the culture method is the gold standard for bacteria identification, it is inefficient, time-consuming (more than 1 week), requires laboratory operator expertise, and identification depends on specific microbiological and biochemical testing [7,9,10]. Besides these methods, there are also other detection methods such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). However, the PCR-based technique requires sophisticated equipment, complicated techniques, and lengthy processes such as ...

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confidence: 99%

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confidence: 99%

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Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus

Astantri

Prakoso

Triyana

et al. 2020

Veterinary Sciences

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“…Listeria genus consists of rod-shape Gram-positive bacteria and includes seventeen different species [123]. Among them, Listeria monocytogenes (LM) is responsible to listeriosis in humans [123] and it was classified as an opportunistic, dangerous pathogen, especially for population high-risk groups such as pregnant women, children, old, and immunosuppressed people. Listeriosis could lead to serious diseases such as meningitis, fetal anomalies, abortion, febrile gastroenteritis or even generalized infection [123].…”

Section: Pathogenic Bacteriamentioning

confidence: 99%

“…Despite the low incidence, when compared to other common foodborne diseases (e.g., Salmonellosis or Escherichia coli infections), LM infection is associated to a greater number of hospitalizations and to a higher mortality rate (20-30%) [123]. In addition, the fact that LM can grow in different food commodities (i.e., dairy products, raw and preserved animal meats and vegetable products) [124] and in adverse environments exacerbates the problem.…”

Section: Pathogenic Bacteriamentioning

confidence: 99%

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

Curulli

2021

Preprint

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Safety and quality are key issues for the food industry. Consequently, there is a growing demand to preserve the food chain and products against substances toxic, harmful to human health such as contaminants, allergens, toxins, or pathogens. For this reason, it is mandatory to develop highly sensitive, reliable, rapid, and cost-effective sensing systems/devices such as electrochemical sensors/biosensors. Generally, conventional techniques are limited by long time of analyses, expensive and complex procedures, and they require skilled personnel. Therefore, the development of performant electrochemical biosensors can significantly support the screening of food chain and products. Here, we report some of the recent developments in this area and analyze the contributions produced by electrochemical biosensors in the food screening and the challenges to address.

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CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

et al. 2022

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Infectious pathogens cause severe human illnesses and great deaths per year worldwide. Rapid, sensitive, and accurate detection of pathogens is of great importance for preventing infectious diseases caused by pathogens and optimizing medical healthcare systems. Inspired by a microbial defense system (i.e., CRISPR/ CRISPR-associated proteins (Cas) system, an adaptive immune system for protecting microorganisms from being attacked by invading species), a great many new biosensors have been successfully developed and widely applied in the detection of infectious viruses and pathogenic bacteria. Moreover, advanced nanotechnologies have also been integrated into these biosensors to improve their detection stability, sensitivity, and accuracy. In this review, the recent advance in CRISPR/Cas systems-based nano/biosensors and their applications in the detection of infectious viruses and pathogenic bacteria are comprehensively reviewed. First of all, the categories and working principles of CRISPR/Cas systems for establishing the nano/biosensors are simply introduced. Then, the design and construction of CRISPR/Cas systems-based nano/biosensors are comprehensively discussed. In the end, attentions are focused on the applications of CRISPR/Cas systems-based nano/biosensors in the detection of infectious viruses and pathogenic bacteria. Impressively, the remaining opportunities and challenges for the further design and development of CRISPR/Cas system-based nano/biosensors and their promising applications are proposed.

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A Minireview of the Methods for Listeria monocytogenes Detection

Cited by 35 publications

References 98 publications

Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus

Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

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