A miR169c-NFYA10 module confers tolerance to low-nitrogen stress to Betula luminifera

Cheng, Lili; Wu, Fangmin; Lin, Yongjun; Han, Xiao; Xu, Xiaoshan; Zhang, Yuting; Yang, Qi; Huang, Hui; Tong, Zaikang; Zhang, Mengjie

doi:10.1016/j.indcrop.2021.113988

Cited by 6 publications

(4 citation statements)

References 61 publications

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“…Then, 27 uniformly growing individuals were selected and randomly divided into two groups, namely, CK and LN. Seedlings in the CK group were cultivated using half-strength liquid MS media including 15 mm KNO 3 , whereas the seedlings in the LN group were cultivated using half-strength MS media containing 0.03 mm KNO 3 (the only source of N), as described previously ( Cheng et al, 2021 ). Then, the leaves and roots were sampled individually after 0.5 and 24 h after the CK and LN treatments, at 11:00 a.m., respectively.…”

Section: Methodsmentioning

confidence: 99%

“…To verify the effectiveness of LN stress treatment, two marker genes NRT2.5 and NRT2.7 were quantified via qRT-PCR ( Supplementary Table S1 ). The large ribosomal subunit 39 ( RPL39 ) was used as a reference gene to normalize the expression levels of marker genes ( Cheng et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…(Supplementary Table S1). The large ribosomal subunit 39 (RPL39) was used as a reference gene to normalize the expression levels of marker genes (Cheng et al, 2021).…”

Section: The Expression Patterns Of Low-nitrogen-responsive Marker Genesmentioning

confidence: 99%

“…TamiR2275 was induced by N starvation stress, which regulated N acquisition and photosynthetic function when plants are challenged by N deprivation ( Qiao et al, 2018 ) . Our previous study demonstrated that a potential regulatory mechanism of the miR169c- NFYA10 module is involved in the low-nitrogen (LN) stress response of B. luminifera ( Cheng et al, 2021 ). However, the expression profiles of miRNAs in response to LN stress are waiting to be elucidated in B. luminifera .…”

Section: Introductionmentioning

confidence: 99%

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Identification of Nitrogen Starvation-Responsive miRNAs to Reveal the miRNA-Mediated Regulatory Network in Betula luminifera

Lin

Chu

et al. 2022

Front. Genet.

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Because of the immobility, plants encounter a series of stresses, such as varied nutrient concentrations in soil, which regulate plant growth, development, and phase transitions. Nitrogen (N) is one of the most limiting factors for plants, which was exemplified by the fact that low nitrogen (LN) has a great adverse effect on plant growth and development. In the present study, we explored the potential role of microRNAs (miRNAs) in response to LN stress in Betula luminifera. We identified 198 miRNAs using sRNA sequencing, including 155 known and 43 novel miRNAs. Among them, 98 known miRNAs and 31 novel miRNAs were differentially expressed after 0.5 h or 24 h of LN stress. Based on degradome data, 122 differential expressed miRNAs (DEmiRNAs) including 102 known miRNAs and 20 novel miRNAs targeted 203 genes, comprising 321 miRNA–target pairs. A big proportion of target genes were transcription factors and functional proteins, and most of the Gene Ontology terms were enriched in biological processes; moreover, one Kyoto Encyclopedia of Genes and Genomes term “ascorbate and aldarate metabolism” was significantly enriched. The expression patterns of six miRNAs and their corresponding target genes under LN stress were monitored. According to the potential function for targets of DEmiRNAs, a proposed regulatory network mediated by miRNA–target pairs under LN stress in B. luminifera was constructed. Taken together, these findings provide useful information to elucidate miRNA functions and establish a framework for exploring N signaling networks mediated by miRNAs in B. luminifera. It may provide new insights into the genetic engineering of the high use efficiency of N in forestry trees.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…(Supplementary Table S1). The large ribosomal subunit 39 (RPL39) was used as a reference gene to normalize the expression levels of marker genes (Cheng et al, 2021).…”

Section: The Expression Patterns Of Low-nitrogen-responsive Marker Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Nitrogen Starvation-Responsive miRNAs to Reveal the miRNA-Mediated Regulatory Network in Betula luminifera

Lin

Chu

et al. 2022

Front. Genet.

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Genome-wide identification and expression analysis of autophagy-related genes (ATGs), revealing ATG8a and ATG18b participating in drought stress in Phoebe bournei

Liu,

Zhang

et al. 2024

Environmental and Experimental Botany

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Genome-wide identification of the bHLH transcription factor family and the regulatory roles of PbbHLH74 in response to drought stress in Phoebe bournei

Fu,

Wang,

Sun

et al. 2024

International Journal of Biological Macromolecules

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A miR169c-NFYA10 module confers tolerance to low-nitrogen stress to Betula luminifera

Cited by 6 publications

References 61 publications

Identification of Nitrogen Starvation-Responsive miRNAs to Reveal the miRNA-Mediated Regulatory Network in Betula luminifera

Identification of Nitrogen Starvation-Responsive miRNAs to Reveal the miRNA-Mediated Regulatory Network in Betula luminifera

Genome-wide identification and expression analysis of autophagy-related genes (ATGs), revealing ATG8a and ATG18b participating in drought stress in Phoebe bournei

Genome-wide identification of the bHLH transcription factor family and the regulatory roles of PbbHLH74 in response to drought stress in Phoebe bournei

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