A mitogenic lectin from Rhizoctonia bataticola arrests growth, inhibits metastasis, and induces apoptosis in human colon epithelial cancer cells

Abstract

The correlation between colorectal cancer (CRC) progression and altered expression of N-glycans can be considered in search for new biomarkers and anticancer agents to control CRC. Earlier N-glycan specific mitogenic lectin from Rhizoctonia bataticola (RBL) has been reported which has growth inhibitory and apoptotic effect on human ovarian and leukemic cells, but mitogenic effect on normal PBMCs revealing its clinical potential. Here, we report the effect of RBL on human colon cancer HT 29, SW480, and SW620 cell growth and its differential binding to human normal colon and cancer tissues. RBL has strong binding to both primary and metastatic colon cancer cells with MFI of 403, 404, and 192, respectively for HT 29, SW480, and SW620 cells. RBL shows dose and time dependent growth inhibitory effect with IC of 5, 6.4, and 6.8 µg/mL, respectively for HT 29, SW480, and SW620 cells. RBL inhibited the clonogenicity of colon carcinoma cells. RBL arrests metastatic SW620 cell growth at S phase, increased hypodiploid population by 6.1%, 14.3%, and 23.2%, respectively at 12, 24, and 36 h. Further, RBL induces SW620 cell apoptosis in time dependent manner, showed increased release of ROS and nuclear degradation compared to lectin untreated control. Adhesion, wound healing, invasion, and migration assays demonstrated anti-metastasis effect of RBL in SW620 cells apart from its growth inhibitory effect. Anti angiogenic effect of RBL was demonstrated by CAM assay. All these results show the promising potential of RBL both as diagnostic and therapeutic agent.

Tepary Bean (Phaseolus acutifolius) Lectins Induce Apoptosis and Cell Arrest in G0/G1 by P53(Ser46) Phosphorylation in Colon Cancer Cells

“…Specifically, in the metastatic SW-620 colon cancer cell line, arrest was found in the S phase and was time-dependent. Arrest was also induced by Rhizoctonia bataticola lectin (RBL) on cells with chromosomic structural loss [31]. Pea lectins provoke differential cell cycle arrest, dependent on the cell line, causing G2/M arrest in SW-48 and G0/G1 arrest in SW-480 cells, both from human colon cancer [32].…”

The fucose‐specific lectin ANL from Aspergillus niger possesses anti‐cancer activity by inducing the intrinsic apoptosis pathway in hepatocellular and colon cancer cells

“…Lectins in general are known to induce cell cycle arrest at either G 0 /G 1 , S, or G 2 /M phases or in combination to induce apoptosis. A noted example is the mitogenic fungal lectin RBL, from Rhizoctonia bataticola , which arrests cell growth of the human metastatic colon cancer cell‐line SW620 increasing the number sub‐G 1 hypodiploid cells within the population in a time‐dependent manner—a typical marker of apoptosis 18,19 . RBL also induces apoptosis in SW620 cells by production of excess ROS which leads to nuclear degradation 18 .…”

Sialic acid-binding lectin-modified fructose-coated nanoparticles: a promising targeted therapeutic synthetic for breast cancers

“…With the biological multifunction such as the insecticide activity, the anti-infection and antitumor capabilities [18], cSBL is currently utilized into the research of treating several types of cancers. The proliferation of tumor cells and tumor angiogenesis can be inhibited when lectins bind to carbohydrate-binding sites and the resultant obstructions in migration and invasion of tumor cells occur [19]. Of note, lectin solely has robust binding capability to primary and metastatic cancer tissues but not to normal human tissues [19], consistent with its outstanding cell killing effects on tumor cells such as breast cancer, cervical cancer, oral cancer, glioblastoma and T cell leukemia but not on healthy cells, for instance, diagonalized cells, fibroblasts and lymphocytes [20][21][22].…”