A mixture of fipronil and fungicides induces alterations on behavioral and oxidative stress parameters in zebrafish

Bevilaqua, Fernanda; Sachett, Adrieli; Chitolina, Rafael; Garbinato, Cristiane; Gasparetto, Henrique; Marcon, Matheus; Mocelin, Ricieri; Dallegrave, Eliane; Conterato, Greicy M. M.; Piato, Ângelo; Siebel, Anna Maria

doi:10.1007/s10646-019-02146-7

Cited by 24 publications

(12 citation statements)

References 58 publications

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“…The reduction in the content of GSH in the kidney and brain of FPN-treated mice (5 and 10 mg/kg bwt) in the same FPN-treated line (5 mg/kg bwt) resulted in a decrease in the concentration of GSH in the testis [ 6 ]. These results are probably attributed to Fipronil’s oxidative anxiety, including reduced GSH levels, and antioxidant activity and consequent lipoperoxidation [ 45 ]. FPN-administered rats showed an overexpression of TNF-α and IL-6 pro-inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 99%

Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats

Tohamy

El-kazaz

Alotaibi

et al. 2021

Animals

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The study investigated the ability of boswellic acid (BA) to alleviate the testicular and oxidative injury FPN insecticide intoxication in the male rat model. Rats were randomly assigned to six equivalent groups (six rats each) as the following: control rats orally administered with 2 mL physiological saline/kg of body weight (bwt); boswellic acid (BA1) rats orally administered 250 mg BA/kg bwt; boswellic acid (BA2) rats orally administered 500 mg BA/kg bwt; fipronil (FPN) rats orally administered 20 mg FPN/kg bwt; (FPN + BA1) rats orally administered 20 mg FPN/kg bwt plus 250 mg BA/kg bwt, and (FPN + BA2) rats orally administered 20 mg FPN/kg bwt plus 500 mg BA/kg bwt. After 60 days, semen viability percentage and live spermatozoa percentage were decreased, and a considerably increased abnormality of the sperm cells in FPN-administered rats improved substantially with the co-administration of BA. BA had refinement of the histological architecture of testes and sexual glands. Quantitative analysis recorded a noticeable decline in the nuclear cell-proliferating antigen (PCNA) percentage area. FPN triggered cell damage, which was suggested by elevated malondialdehyde and interleukin 6, tumor necrosis factors alpha, and decreased glutathione level. Proapoptotic factor overexpression is mediated by FPN administration, while it decreased the antiapoptotic protein expression. Similarly, BA has shown significant upregulation in steroidogenic and fertility-related gene expression concerning the FPN group. Pathophysiological damages induced by FPN could be alleviated by BA’s antioxidant ability and antiapoptotic factor alongside the upregulation of steroidogenic and fertility-related genes and regimented the detrimental effects of FPN on antioxidant and pro-inflammatory biomarkers.

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Section: Discussionmentioning

confidence: 99%

Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats

Tohamy

El-kazaz

Alotaibi

et al. 2021

Animals

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“…Some authors indicated that this compound is highly toxic to zebrafish larvae (Xu et al 2018 ; Park et al 2020 ) and a fish species Aristichthys nobilis , possibly due to high affinity of GABA receptors (Zhang et al 2018 ). In zebrafish embryos, larvae and adults adverse changes of behavioral parameters and oxidative stress were also found (Stehr et al 2006 ; Bevilaqua et al 2020 ). FIP also induces toxic effects in marine and freshwater crustaceans such as estuarine copepod Amphiascus tenuiremis (Chandler et al 2004 ), kuruma prawn, sand shrimp, and a surrogate mysid species Americamysis bahia (Hano et al 2019 ), blue crab Callinectes sapidus (Goff et al 2017 ), juvenile brown shrimp Farfantepenaeus aztecus (Al-Badran et al 2018 ), and grass shrimp Palaemonetes pugio (Key et al 2003 ).…”

Section: Introductionmentioning

confidence: 99%

Short-term effects of pesticide fipronil on behavioral and physiological endpoints of Daphnia magna

Bownik

Szabelak

2021

Environ Sci Pollut Res

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Fipronil (FIP) is an organic pesticide with many practical uses. Although some results indicated toxic effects in some terrestrial and aquatic animal species, little is known on its influence on behavioral and physiological endpoints of cladocerans. The aim of our study was to determine the short-term effects of FIP at concentrations of 0.1 μg/L, 1 μg/L, 10 μg/L, and 100 μg/L on Daphnia magna sublethal indices: behavioral (swimming speed, distance traveled) and physiological endpoints (heart rate, post-abdominal claw activity and thoracic limb movements). The results showed that FIP induced reduction of swimming speed and distance traveled in a concentration- and time-dependent manner at all the concentrations used. The lowest concentration of the insecticide temporarily stimulated post-abdominal claw activity after 24 h and thoracic limb activity after 48 h; however, the highest concentrations reduced all the studied physiological endpoints. IC50 values showed that thoracic limb activity, swimming speed, and distance traveled were most sensitive to FIP after 24-h exposure. The most sensitive parameter after 48 h and 72 h was swimming speed and post-abdominal claw activity, respectively. The study indicated that (i) behavioral and physiological endpoints of Daphnia magna are reliable and valuable sublethal indicators of toxic alterations induced by FIP; however, they respond with different sensitivity at various times of exposure, (ii) FIP may alter cladoceran behavior and physiological processes at concentrations detected in the aquatic environment; therefore, it should be considered as an ecotoxicological hazard to freshwater cladocerans.

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“…The approval of new pesticides by MAPA requires knowledge of their isolated effects and possible interactions in tank mixtures. Pesticide mixtures have been used to pest control simultaneously and reduce management costs [6,7]. Legalizing this practice occurred with the Normative Instruction No.…”

Section: Introductionmentioning

confidence: 99%

“…The risks associated with tank mixtures mainly concern physical and chemical incompatibility and knowledge of synergistic, antagonistic, and additive interactions among the compounds [9]. In addition, the pursuit of sustainable production in the forestry sector lacks research on the impact of pesticide mixtures [7].…”

Section: Introductionmentioning

confidence: 99%

Expansion of Planted Forests: The Risk of Pesticides Mixtures

Barroso

Duque

Custódio

et al. 2022

Forests

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Planted forests include forests established through human planting or deliberate seeding. They are systems that offer us timber and non-timber forest products and ecosystem services, such as wildlife protection, carbon sequestration, soil, and watershed maintenance. Brazil has 7.6 million hectares of planted forests, with 72% of the total area occupied by Eucalyptus spp. A favorable climate and management and genetic improvement research are the main factors responsible for high productivity. In recent years, the expansion of planted areas has been accompanied by the commercial release of several pesticides, mainly herbicides. A recent change in the Brazilian legislation allows mixing phytosanitary products in a spray tank, having a new approach to managing pests, diseases, and weeds. Antagonism is the main risk of tank mixes, and to reduce the dangers associated with this practice, we review all products registered for growing Eucalyptus. This literature review aims to identify the effects of product mixtures registered for Eucalyptus reported for other crops. In addition, environmental and social risk assessment has been widely adopted to export wood and cellulose, making the results of this review an indispensable tool in identifying the nature and degree of risks associated with pesticides. The results classify the effects of the mixtures as an additive, antagonistic or synergistic. The use of pesticide tank mixtures has the potential for expansion. However, there are still challenges regarding variations in the effects and applications in different climatic conditions. Therefore, studies that prove efficient mixtures for the forest sector are essential and the training of human resources.

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A mixture of fipronil and fungicides induces alterations on behavioral and oxidative stress parameters in zebrafish

Cited by 24 publications

References 58 publications

Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats

Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats

Short-term effects of pesticide fipronil on behavioral and physiological endpoints of Daphnia magna

Expansion of Planted Forests: The Risk of Pesticides Mixtures

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