A model for baculovirus production with continuous insect cell cultures

Gooijer, C.D. de; Lier, F.L.J. van; End, E.J. van den; Vlak, Just M.; Tramper, J.

doi:10.1007/bf00263855

Cited by 38 publications

(11 citation statements)

References 9 publications

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“…min), which depends on the cell line. This expression for the infection rate is a more general form than originally given by de Gooijer et al (1989), who assumed viral infection was first order with cell concentration when non-occluded infectious virus was in excess. When virus is not in excess, the infection rate also depends first order on viral concentration.…”

Section: Infection Rates Of Different Cell Linesmentioning

confidence: 85%

“…Tn 5B1-4 and Tn F cells showed the highest infection rates (ki = 4.4 x 10 -9 cell/ml, min) compared to the cell lines Sf-21, Sf-9 and Tn 368 which showed infection rates five-to 10-fold lower. Detailed comparison between our measured infection rates and those determined by de Gooijer et al (1989) is not possible without detailed knowledge of viral concentrations in their experiments. * Measured using the number of plaques formed at 5 days after a 15 min incubation at 22 °C with 5000 p.f.u./ml and 106 cells/ml.…”

Section: Infection Rates Of Different Cell Linesmentioning

confidence: 99%

See 1 more Smart Citation

Equilibrium and kinetic analysis of Autographa californica nuclear polyhedrosis virus attachment to different insect cell lines

Wickham¹,

Shuler²,

Hammer³

et al. 1992

Journal of General Virology

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The kinetic and equilibrium attachment of Autographa californica nuclear polyhedrosis virus (AcMNPV) to seven insect cell lines was evaluated. Kinetic experiments revealed differences of up to 10-fold in the infection rates among cell lines. Equilibrium binding also varied between cell lines and was saturable. The Tn 5B1-4 and Tn F cell lines had the highest virus binding affinities and infection rates and exhibited diffusion-limited attachment. The rate of infection appears to be limited by the rate of attachment. For the Tn 5B1-4 cells the physical to infective particle ratio for AcMNPV was 5-3. From the Scatchard analyses, the cell lines Tn 5BI-4 and Tn F displayed affinities of 2-35x 10 L° M -1 and 1-60x 101° M -1, respectively, with 6000 and 13 700 binding sites per cell. The insect cell line Hz 1075, which is not susceptible to AcMNPV infection, displayed a much lower, but saturable, binding of AcMNPV with 900 sites/cell and an affinity of 1-1xl01° M -1. Unlabelled AcMNPV, but not Lymantria dispar MNPV could compete with labelled AcMNPV for binding sites. There were 93 to 96% reductions in virus cell binding following pretreatments of cells with three proteases, suggesting the involvement of a cellular protein component in virus binding. Tunicamycin, an inhibitor of N-linked glycosylation and expression of some membrane proteins on the cell surface, reduced virus binding in a dose-dependent manner suggesting a role for glycoprotein(s) in binding. However there was no evidence for the direct involvement of oligosaccharides in attachment. Metabolic inhibitors of oligosaccharide trimming and competition binding assays using simple sugars caused no measurable reductions in virus binding. These findings suggest that AcMNPV attachment to insect cells is receptor-mediated via a glycoprotein component(s); the direct involvement of oligosaccharide moieties in binding is unlikely.

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Section: Infection Rates Of Different Cell Linesmentioning

confidence: 85%

Section: Infection Rates Of Different Cell Linesmentioning

confidence: 99%

Equilibrium and kinetic analysis of Autographa californica nuclear polyhedrosis virus attachment to different insect cell lines

Wickham¹,

Shuler²,

Hammer³

et al. 1992

Journal of General Virology

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show abstract

“…This requires the design and optimization of bioreactors of relatively large volume, and the optimization of their operation strategy. Mathematical modeling has been an important tool in this task (de Gooijer et al, 1989;Kumar & Shuler, 1995;Power & Nielsen, 1996).…”

Section: Significance Of Baculovirus-insect Cell Culture Systemmentioning

confidence: 99%

Optimization of the Strategy for Recombinant Baculovirus Infection of Suspended Insect Cells

Zhou¹,

Zhang²

2011

Pesticides in the Modern World - Pests Control and Pesticides Exposure and Toxicity Assessment

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“…To infect a cell an NOY enters the cell by endocytosis or fusion (12). The viral DNA is replicated in the nucleus of the cell and about 12-14 hours post infection virus particles are released from the cell (13,14). NOYs are responsible for the spread of the infection to other cells.…”

Section: Replicationmentioning

confidence: 99%

“…Based on their model they calculated that the number of receptors on Sf -9 cells ranges from 10 5 to 10 7 per cell. The affinity of the virus to the receptors was predicted to be in the range 10 4 -10 5 M-1 • The infection rate has been proposed to follow first-order kinetics with regard to viableinsect-cell concentration (14,28). With this assumption and using cell balances De Gooijer et al (14) calculated a first-order reaction constant in the order of 1.1O-6 .s-1 using data from continuous bioreactor systems.…”

Section: S Modellingmentioning

confidence: 99%