A model for the study of lymph node metastasis from oral carcinoma by serial transplantation of metastatic tumor in hamsters

Expression of Adhesion Molecules in the Vascular Invasion Process of O-1N, Hamster Squamous Cell Carcinoma with High Potential of Lymph Node Metastasis.

“…The tumor used is a squamous cell carcinoma termed O-lN that has been maintained for over 60 generations by serial transplantation of lymph node metastases into the buccal pouch in hamsters. The original tumor is a 9,10-dimethyl-1,2-benzanthracene-induced squamous cell carcinoma of the tongue, inducing metastases in the submandibular lymph node [2]. Metastatic tumors of the submandibular lymph nodes were removed from donor hamsters and minced into small pieces of approximately 2 mm 3 in size under aseptic conditions.…”

“…Thus, lymph node metastasis is the most significant factor that affects the prognosis of patients with oral cancer [1]. In order to elucidate the mechanism of metastasis of oral carcinomas, we have established an animal model of lymph node metastasis (termed O-lN) by serial transplantation of a metastatic tumor to the submandibular lymph node from a chemically induced squamous cell carcinoma of the tongue into the buccal pouch of hamsters [2]. This model proved to be quite reliable because of the high and constant rate of nodal metastasis and the close resemblance to human oral carcinomas in the process of metastasis [3].…”

Detection of Hamster Squamous Cell Carcinoma Cells(O-1N) in Blood and Lymph Nodes by Reverse Transcriptase-Polymerase Chain Reaction for Cytokeratin 19.

“…In order to elucidate the mechanism of metastasis of oral carcinomas, we have established an animal model of lymph node metastasis (termed O-1 N) by serial transplantation of a metastatic tumor to the submandibular lymph node from a chemically induced squamous cell carcinoma of the tongue into the buccal pouch in hamsters (3). This model proved to be quite reliable because of the high and constant rate of nodal metastasis and the close resemblance to human oral carcinomas in the process of metastasis (4).…”