A model for transcription termination by RNA polymerase I

Lang, Walter H.; Morrow, Bernice E.; Ju, Qida; Warner, Jonathan R.; Reeder, Ronald H.

doi:10.1016/0092-8674(94)90261-5

Cited by 99 publications

(133 citation statements)

References 27 publications

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“…Then Ϸ90% of transcripts terminate before probe r4 with little signal detectable above background levels beyond probe r5. These TRO signals closely match the in vitro and steady-state in vivo data previously obtained for Pol I termination (11,15). It should be noted that during the TRO reaction, it is likely that Pol I will extend the transcript position by several hundred nucleotides (29).…”

Section: Resultssupporting

confidence: 87%

“…In contrast, Pol I termination in yeast has been shown to use a DNA-binding factor, Reb1p, which identifies a well defined sequence element at the 3Ј ends of the primary Pol I transcription unit. Reb1p then promotes termination just upstream, over a T rich sequence that appears to operate as a polymerase release element (11,12). In higher eukaryotes, a specific release factor has been identified (13), whereas in yeast, a similar release process may occur, although no release factor has so far been identified (14).…”

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confidence: 99%

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Transcriptional termination by RNA polymerase I requires the small subunit Rpa12p

Prescott

Osheim

Jones

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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We identify Rpa12p of RNA polymerase I (Pol I) as a termination factor. Combined analyses using transcription run-on, electron microscopy-visualized chromatin spreading and RT-PCR have been applied to the rRNA-encoding genes of Saccharomyces cerevisiae. These confirm that Pol I termination occurs close to the Reb1p-dependent terminator in wild-type strains. However, deletion mutants for the 3 end-processing enzyme Rnt1p or the Rpa12p subunit of Pol I both show Pol I transcription in the spacer. For ⌬rpa12, these spacer polymerases are devoid of nascent transcripts, suggesting they are immediately degraded. The homology of Rpa12p to the small subunit Rpb9p of Pol II and Rpc11p of Pol III, both implicated in transcriptional termination, points to a common termination mechanism for all three classes of RNA polymerase.

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Section: Resultssupporting

confidence: 87%

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confidence: 99%

Transcriptional termination by RNA polymerase I requires the small subunit Rpa12p

Prescott

Osheim

Jones

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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“…Another sequence element situated upstream of the Reb1-binding site in yeast and the Sal box in mammals, which is necessary for termination, acts as a transcript release element (Lang et al 1994;Jeong et al 1995;Lang and Reeder 1995). This sequence is composed of a stretch of Ts.…”

Section: Rnapi Terminationmentioning

confidence: 99%

“…The weakness of the resulting AÁU heteroduplex might destabilize RNAPI pausing at the Reb1/TTF-Imediated pause site (Kuhn and Grummt 1989;Lang and Reeder 1995). Consequently, rRNA precursors terminated at T1 are formed ;12-20 bp upstream of the Reb1-binding site and within the T stretch, 93 nt downstream from the mature 39-end of 25S RNA (Lang et al 1994;Jeong et al 1995).…”

Section: Rnapi Terminationmentioning

confidence: 99%

Transcription termination by nuclear RNA polymerases

Richard

Manley²

2009

Genes Dev.

291

326

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Gene transcription in the cell nucleus is a complex and highly regulated process. Transcription in eukaryotes requires three distinct RNA polymerases, each of which employs its own mechanisms for initiation, elongation, and termination. Termination mechanisms vary considerably, ranging from relatively simple to exceptionally complex. In this review, we describe the present state of knowledge on how each of the three RNA polymerases terminates and how mechanisms are conserved, or vary, from yeast to human.

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“…Thus, the rDNA promoter has been precisely located upstream from the TIS and protein factors that control the rDNA promoter activity have been identified (Moss and Stefanovsky, 1995). In a similar manner rDNA terminators have been located in the IGS downstream from the 25s coding sequence, and factors that interact with this element have been identified in mammals (Evers et al, 1995) and in yeast (Lang et al, 1994).…”

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confidence: 97%

Two Ribosomal DNA‐Binding Factors Interact with a Cluster of Motifs on the 5′ External Transcribed Spacer, Upstream from the Primary Pre‐rRNA Processing Site in a Higher Plant

Caparrós-Ruiz

Lahmy

Piersanti

et al. 1997

European Journal of Biochemistry

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In radish the primary processing site in pre-rRNA has been mapped to a TTTTCGCGC sequence Nuclear genes coding for the 25S, 5.8s and 18s rRNAs are organised as head-to-tail tandem repeats of the rRNA sequences separated by two short spacers and a large intergenic spacer (IGS). Transcription of this unit by RNA polymerase I gives a primary rRNA precursor (pre-rRNA) comprising the rRNA and transcribed spacer sequences. Pre-rRNA processing results in removal of spacer sequences by a combination of endonucleolytic cleavages at specific sites, exonucleolytic RNA degradation, and base modification of rRNAs. All these events occur in the nucleolus (Sollner-Webb and Mougey, 1991 ;Moss and Stefanovsky, 1995).In eucaryotes, ribosomal DNA (rDNA) transcription starts at a major transcription initiation site (TIS) located in the IGS and proceeds through the 5' external transcribed spacer (5' ETS). The first pre-rRNA-processing event occurs in the nascent transcript and corresponds to an endonucleolytic cleavage at a speCorrespondence to M. Echevem'a,

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A model for transcription termination by RNA polymerase I

Cited by 99 publications

References 27 publications

Transcriptional termination by RNA polymerase I requires the small subunit Rpa12p

Transcriptional termination by RNA polymerase I requires the small subunit Rpa12p

Transcription termination by nuclear RNA polymerases

Two Ribosomal DNA‐Binding Factors Interact with a Cluster of Motifs on the 5′ External Transcribed Spacer, Upstream from the Primary Pre‐rRNA Processing Site in a Higher Plant

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