A model for xenotransplantation of human malignant astrocytomas into the brain of normal adult rats

Lg, Strömblad; Brun, Arne; Lg, Salford; Stenevi, Ulf

doi:10.1007/bf01405848

Cited by 13 publications

(4 citation statements)

References 9 publications

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“…Attempts to mimick natural heterogeneity by coinoculation experiments performed with A15A5 and F98, both nitroso-methylurea-induced glioma cell lines in BDIX and Fisher rats respectively, have failed (Whittle et al 1998). As established in several studies intracranial inoculation in nude rats of spheroids of tumor cells from human biopsy specimens or human tumors xenografted into an animal model maintains more features of the original tumor than tumors derived from cell lines (Strömblad et al 1982;Engebraaten et al 1999). …”

Section: Morphology Of C6-derived Tumorsmentioning

confidence: 45%

Rat C6 glioma as experimental model system for the study of glioblastoma growth and invasion

Grobben

Deyn

Slegers

2002

Cell and Tissue Research

318

247

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Infiltration of the central nervous system by neoplastic cells in patients with glioblastoma multiforme (GBM) leads to neurological dysfunction and eventually to death. The elucidation of the mechanisms underlying the aggressive nature of GBM aims at improving radio-, chemo- and gene therapy. This review is focused on the use of rat C6 glioma as an experimental model system for GBM and provides an overview of the experimental data published in the literature using this cell line in elucidating the mechanism of tumor growth, angiogenesis and invasion, and in the design and evaluation of anticancer therapies. Understanding the stages of malignant brain tumor progression requires a series of experimental approaches with a varying degree of complexity. Implantation of malignant cells into animal brain tissue closely resembles in vivo tumor growth and has the advantage over simplified models that inflammatory and vascular mechanisms are activated. However, the complexity of these models makes it difficult to identify the individual processes involved in sustained tumor growth, angiogenesis and invasion. In cell culture models, the effect of growth factors, extracellular matrix components, proteases and adhesion molecules can be investigated. The secretion of tumor-derived factors into the medium can also be analyzed when simplified models are used. This review is a compilation of experimental data focused on the characterization of tumor-related processes and on the evaluation of new therapies for the treatment of malignant glial neoplasms using rat C6 glioma as a model system.

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Section: Morphology Of C6-derived Tumorsmentioning

confidence: 45%

Rat C6 glioma as experimental model system for the study of glioblastoma growth and invasion

Grobben

Deyn

Slegers

2002

Cell and Tissue Research

318

247

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“…40 Direct implantation of glioma fragments after careful verification of their tumor-cell content has been successfully used in in vivo invasion studies in which human astrocytomas were grafted into the rat brain. 29,51 Our results indicate that, with an appropriate invasion substrate, selected fragments can be used in an ex vivo invasion assay. …”

Section: Importance Of Avoiding Preculturing Stepsmentioning

confidence: 44%

Invasion of human glioma biopsy specimens in cultures of rodent brain slices: a quantitative analysis

Boüard¹,

Christov²,

Guillamo³

et al. 2002

Journal of Neurosurgery

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“…Despite these advantages, nude rats have been used only to a limited extent for experimental studies on human cancers, and only a few reports have documented xenotransplantation of human tumors into the rat brain, e.g. glioma [15], astrocytoma [16] and SCLC [17]. In view of future applications of monoclonal antibodies (moab's) in the detection of cerebral me-tastases of SCLC it would be important to know the penetration of moab's into the cerebral outgrowths of SCLC.…”

Section: Introductionmentioning

confidence: 45%

Intracerebral and subcutaneous xenografts of human SCLC in the nude rat: comparison of monoclonal antibody localization and tumor infiltrating lymphocytes

et al. 1993

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In the WAG/Rij nude rat, subcutaneous (s.c.) and intracerebral (i.c.) xenografts of the human SCLC cell line GLC-28 were evaluated for their growth behavior, in vivo monoclonal antibody binding and presence of tumor infiltrating lymphocytes. For the i.c. xenografts, two models of cerebral tumor growth were studied, one in the cerebral cortex and one in the lateral ventricle of the brain. In the s.c. and both i.c. xenografts models, in vivo localization of anti-carcinoma moab MOC-31 occurred within 4 hours after i.p. injection, with a maximal binding at 24 h after injection. A pronounced tumor infiltration of predominantly NK cells was observed for s.c. and intraventricular xenografts, but not for the GLC-28 tumors xenografted in the cerebral cortex. The presented nude rat/GLC-28 xenograft models may be used for the in vivo testing of experimental imaging techniques or alternative treatment strategies relevant to brain metastases of human SCLC.

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A model for xenotransplantation of human malignant astrocytomas into the brain of normal adult rats

Cited by 13 publications

References 9 publications

Rat C6 glioma as experimental model system for the study of glioblastoma growth and invasion

Rat C6 glioma as experimental model system for the study of glioblastoma growth and invasion

Invasion of human glioma biopsy specimens in cultures of rodent brain slices: a quantitative analysis

Intracerebral and subcutaneous xenografts of human SCLC in the nude rat: comparison of monoclonal antibody localization and tumor infiltrating lymphocytes

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