A model of chronic IgE-mediated food allergy in ovalbumin-sensitized mice

Saldanha, J.C.S.; Gargiulo, Daniela Longo; Silva, S.S.; Carmo-Pinto, F.H.; Andrade, Mariléia Cháves; Alvarez-Leite, Jacqueline I.; Teixeira, Mauro Martins; Cara, Denise Carmona

doi:10.1590/s0100-879x2004000600005

Cited by 62 publications

(45 citation statements)

References 20 publications

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“…Also the positive controls developed high levels of all antibody subclasses, which is in line with previous studies [31,32]. Comparable to this route, even subcutaneous immunizations with OVA adsorbed to aluminum hydroxide were reported to induce high levels of IgG1 and IgE [33,34]. Furthermore, in our experiments both immunization regimens, i.p.…”

Section: Discussionsupporting

confidence: 92%

Dose-dependent food allergy induction against ovalbumin under acid-suppression: A murine food allergy model

et al. 2008

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Background-Animal models are essential for analyzing the allergenic potential of food proteins and for investigating mechanisms underlying food allergy. Based on previous studies revealing acid-suppression medication as risk factor for food allergy induction, we aimed to establish a mouse model mimicking the natural route of sensitization in patients.Methods-The effect of acid-suppressing medication on murine gastric pH was assessed by intragastric pH measurements after two injections of a proton pump inhibitor (PPI). To investigate dose-dependency, mice were fed different concentrations of ovalbumin (OVA; 0.2, 0.5, 1.0, 2.5 or 5.0 mg) either with or without anti-ulcer medication. Additionally, different routes of exposure (i.p. vs. oral) were compared in a second immunization experiment. Sera were screened for OVAspecific antibody titers (IgG1, IgG2a and IgE) in ELISA and RBL assay. Clinical reactivity was evaluated by measuring rectal temperature after oral challenge and by type I skin tests.Results-Two intravenous injections of PPI significantly elevated the gastric pH from 2.97 to 5.3. Only oral immunization with 0.2 mg OVA under anti-acid medication rendered elevated IgG1, IgG2a and IgE titers compared to all other concentrations. Protein feeding alone altered antibody titers only marginally. Even though also i.p. immunizations induced high levels of specific IgE, only oral immunizations under anti-acids induced anaphylactic reactions evidenced by a significant decrease of body temperature.Conclusion-Only low-dosage ovalbumin feedings under anti-acid medication resulted in IgE mediated food allergy. Based on this knowledge we have established a suitable food allergy model for further investigations of food adverse reactions.

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Section: Discussionsupporting

confidence: 92%

Dose-dependent food allergy induction against ovalbumin under acid-suppression: A murine food allergy model

et al. 2008

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“…20 Cobalt-or vehicle-treated rats were injected with 20 mg/kg Evans blue via tail vein. After 20 min, kidneys were removed and the cortex dissected.…”

Section: Evaluation Of Vascular Permeabilitymentioning

confidence: 99%

Cobalt promotes angiogenesis via hypoxia-inducible factor and protects tubulointerstitium in the remnant kidney model

Tanaka

Kojima

Ohse

et al. 2005

Laboratory Investigation

220

141

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Tubulointerstitial hypoxia has been implicated in a number of progressive renal diseases, and several lines of evidence indicate that the administration of angiogenic growth factors ameliorates tubulointerstitial injury. We hypothesized that induction of hypoxia-inducible factors (HIF) mediates renoprotection by their angiogenic properties. At 5-9 weeks after subtotal nephrectomy, cobalt was administered to rats to activate HIF. Histological evaluation demonstrated that the tubulointerstitial injury was significantly ameliorated in animals that received cobalt (score: 2.5170.12 (cobalt) vs 3.2170.24 (vehicle), Po0.05). Furthermore, animals receiving cobalt had fewer vimentin-and TdT-mediated dUTP nick-end labeling (TUNEL)-positive tubular cells. The renoprotective effect of cobalt was associated with the preservation of peritubular capillary networks (rarefaction index: 13.770.4 (cobalt) vs 18.670.9 (vehicle), Po0.01). This improvement in capillary networks was accompanied by an increased number of proliferating (PCNA-positive) glomerular and peritubular endothelial cells. The angiogenesis produced by this method was not accompanied by an increase in vascular permeability. Furthermore, in vitro experiments clarified that HIF-1 in tubular epithelial cells promotes proliferation of endothelial cells and that HIF-2 overexpressed in renal endothelial cells mediates migration and network formation. Collectively, these findings demonstrate a renoprotective role of HIF through angiogenesis and provide a rationale for therapeutic approaches to target HIF for activation.

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“…Therefore, our group has developed a murine model of food allergy to ovalbumin (OVA) in which sensitized mice are challenged orally for 7 days. In this model, several signals similar to those that occur in patients with food allergy are developed, such as antigenic aversion, increased anti-OVA IgE production, intestinal oedema and intestinal eosinophil infiltration, as well as a marked weight and adipose tissue loss [13,14]. In addition, we have demonstrated that this allergic process induces an adipose tissue inflammation with an increase in the number of inflammatory cells and proinflammatory cytokine levels in this tissue, resulting in systemic metabolic alterations including the decrease in the serum glucose, triglycerides and total cholesterol [14].…”

Section: Introductionmentioning

confidence: 99%

Prolonged ingestion of ovalbumin diet by sensitized mice improves the metabolic consequences induced by experimental food allergy

Batista¹,

Pereira²,

Noviello³

et al. 2014

Clinical and Experimental Immunology

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SummaryThe prevalence of food allergy is rising in the western world. Allergen restriction is the chosen treatment in this condition, but continuous ingestion of the antigen has shown positive results in clinical trials. In a previous study, we have shown several allergic and metabolic alterations after 7 days of ovalbumin (OVA) ingestion by sensitized mice. The aim of this study was to investigate whether prolonged ingestion of antigen by sensitized mice would reverse the metabolic consequences caused by experimental food allergy. For this, allergic and metabolic parameters were analysed after prolonged ingestion of an OVA diet by OVA-sensitized mice. As shown previously, after 7 days of OVA consumption, sensitized mice showed increased serum levels of anti-OVA immunoglobulin (Ig)E and IgG1, aversion to the antigen ingestion, marked body and adipose tissue weight loss, followed by adipose tissue inflammation and decreased serum levels of adipokines, glucose and triglycerides. However, after 14 days of oral challenge, sensitized mice showed an anti-OVA IgE level similar to the mice that were only sensitized, but the specific IgG1 did not change. With this prolonged ingestion of OVA, sensitized mice were protected from OVA-induced anaphylaxis when the antigen was given systemically at a dose of 2 mg/animal. Moreover, various parameters analysed were significantly ameliorated, including adipose tissue inflammation, body and adipose tissue loss, as well as serum levels of adipokines and triglycerides. Therefore, our data suggest that prolonged ingestion of OVA by sensitized mice results in an improvement of the metabolic consequences caused by experimental food allergy.

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A model of chronic IgE-mediated food allergy in ovalbumin-sensitized mice

Cited by 62 publications

References 20 publications

Dose-dependent food allergy induction against ovalbumin under acid-suppression: A murine food allergy model

Dose-dependent food allergy induction against ovalbumin under acid-suppression: A murine food allergy model

Cobalt promotes angiogenesis via hypoxia-inducible factor and protects tubulointerstitium in the remnant kidney model

Prolonged ingestion of ovalbumin diet by sensitized mice improves the metabolic consequences induced by experimental food allergy

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