A Model of Human Acute Lymphoblastic Leukemia in Immune-Deficient SCID Mice

Kamel‐Reid, Suzanne; Letarte, Michelle; Sirard, Christian; Doedens, Monica; Grunberger, Tom; Fulop, G. M.; Freedman, Melvin H.; Phillips, Robert A.; Dick, John E.

doi:10.1126/science.2595371

Cited by 229 publications

(105 citation statements)

References 33 publications

Supporting

Mentioning

103

Contrasting

Order By: Relevance

“…Previous studies of human leukemias comparing Nude and SCID mice demonstrated the superiority of SCID mice in supporting the growth of BALL-1, Molt-4, CEM and A-1 human leukemia cell lines. 14,18,26 The results of these studies were very similar to those of the present investigations which showed the superiority of SCID over Nude mice in the growth of each of the cell lines examined.…”

Section: Discussionsupporting

confidence: 87%

Xenotransplantation of human lymphoid malignancies is optimized in mice with multiple immunologic defects

Hudson

et al. 1998

Leukemia

View full text Add to dashboard Cite

While it is known that mice with genetic immune defects are useful for establishing durable engraftment of human tumor xenografts, the relative role of components of host innate and adoptive immunity in engraftment has not been determined. We directly compared the ability of four strains of genetically immunodeficient mice (NOD/SCID, SCID, Nude and Rag-1-deficient) to successfully engraft and support the human cell lines Daudi, Raji, Namalwa and Molt-4 as subcutaneous tumors. We additionally examined the effect of further immunosuppression of the mice by whole body irradiation at a dose of 600 cGy for Nude and Rag-1 and 300 cGy for SCID mice and by administration of anti-natural killer (asialo-GM1) antibody on tumor growth. Mice with each of the defects supported xenografts to varying degrees. We found differences in growth characteristics in the cell lines tested, with Namalwa consistently producing the largest tumors. With all cell lines studied, optimal growth was achieved using NOD/SCID mice. Overall, tumor growth was somewhat enhanced by pretreatment with radiation with little additional benefit from the addition of anti-asialo-GM1 antibody. The importance of multiple components of the innate and adoptive immune system in xenotransplantation were best demonstrated when results in untreated NOD/SCID mice were compared to SCID, nude and RAG-1-deficient mice. The NOD/SCID mouse with or without additional immunosuppression provides the optimal model for the study of the biology and treatment of human leukemias and lymphomas.

show abstract

Section: Discussionsupporting

confidence: 87%

Xenotransplantation of human lymphoid malignancies is optimized in mice with multiple immunologic defects

Hudson

et al. 1998

Leukemia

View full text Add to dashboard Cite

show abstract

“…Experimental evidence for human CSCs is considered to be best established by demonstrating the ability of cells that are directly isolated from patients to produce malignant daughter populations in transplanted immunodeficient mice. This approach was pioneered with samples of cells from patients with various types of leukaemia (acute lymphoid leukaemia (ALL) 31 , acute myeloid leukaemia (AML) 32 and chronic myeloid leukaemia (CML) 33 ). Subsequently, the same approach has been successfully applied to solid tumours, including human cancers arising in the breast 34 , brain 14 , colon 16,35 , ovary 36 , lung 17 , and head and neck 37 .…”

Section: When a Clonal Hierarchy Is In Questionmentioning

confidence: 99%

Cancer stem cell definitions and terminology: the devil is in the details

Valent

Bonnet

Maria³

et al. 2012

Nat Rev Cancer

625

564

View full text Add to dashboard Cite

| The cancer stem cell (CSC) concept has important therapeutic implications, but its investigation has been hampered both by a lack of consistency in the terms used for these cells and by how they are defined. Evidence of their heterogeneous origins, frequencies and their genomic, as well as their phenotypic and functional, properties has added to the confusion and has fuelled new ideas and controversies. Participants in The Year 2011 Working Conference on CSCs met to review these issues and to propose a conceptual and practical framework for CSC terminology. More precise reporting of the parameters that are used to identify CSCs and to attribute responses to them is also recommended as key to accelerating an understanding of their biology and developing more effective methods for their eradication in patients. PERSPECTIVES NATURE REVIEWS | CANCER VOLUME 12 | NOVEMBER 2012 | 767

show abstract

“…To confirm our in vitro results in the human in vivo system, we used mice with severe combined immunodeficiency (SCID). Recent studies have demonstrated that the SCID mouse has several defects in both humoral and cellmediated immunity, and can serve as a recipient of grafts derived from a variety of human tissues, [22][23][24][25][26][27] including rheumatoid synovium. Thus, proliferative rheumatoid synovium can grow as a xenograft in SCID mice and pre- serve the histologic components of rheumatoid synovia.…”

Section: Figure 4 Immunohistochemical Analysis Of the Effects Of Hfasmentioning

confidence: 99%

Induction of apoptosis in the rheumatoid synovium by Fas ligand gene transfer

et al. 1998

View full text Add to dashboard Cite

We have recently reported that local administration of antiactivity against RA synoviocytes via the Fas/FasL system Fas monoclonal antibody (MAb) in human T cell leukemia in vitro. Histopathological and immunohistochemical studvirus type 1 (HTLV-1) carrying mice improved arthritis due ies showed that local injection of irradiated-hFasL transfecto the induction of apoptosis. This finding strongly indicated tants eliminated synoviocytes and mononuclear cells in the beneficial therapeutic effect of Fas-mediated apoptosis engrafted human rheumatoid synovium of SCID-RA mice. in rheumatoid arthritis (RA). To establish further the theraFurthermore, in situ nick end labeling analysis confirmed peutic effect of Fas-mediated apoptosis on RA taking into that the cells in engrafted synovium frequently underwent consideration safety and practicality, we investigated the apoptosis by irradiated-hFasL transfectants. Our results effect of cells transfected with human Fas ligand (hFasL) clearly demonstrated that hFasL transfectants induced gene on proliferating human rheumatoid synovium apoptosis by cell-to-cell interaction via the Fas/FasL sysengrafted in severe combined immunodeficiency (SCIDtem. Thus, ex vivo gene transfer of FasL may represent a RA) mice. The hFasL transfectants exhibited cytotoxic novel therapeutic strategy for RA.

show abstract

A Model of Human Acute Lymphoblastic Leukemia in Immune-Deficient SCID Mice

Cited by 229 publications

References 33 publications

Xenotransplantation of human lymphoid malignancies is optimized in mice with multiple immunologic defects

Xenotransplantation of human lymphoid malignancies is optimized in mice with multiple immunologic defects

Cancer stem cell definitions and terminology: the devil is in the details

Induction of apoptosis in the rheumatoid synovium by Fas ligand gene transfer

Contact Info

Product

Resources

About