A moderate protective effect of quercetin against γ-irradiation- and storage-induced oxidative damage in red blood cells for transfusion

Żbikowska, Halina Małgorzata; Antosik, Adam; Szejk, Magdalena; Bijak, Michał; Nowak, Paweł

doi:10.3109/09553002.2013.877173

Cited by 11 publications

(7 citation statements)

References 47 publications

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“…These events were already seen and widely discussed in our earlier studies [14,23]. During storage, oxidative damage to lipids and proteins contributes to RBC injury.…”

Section: Discussionsupporting

confidence: 69%

“…One approach to improve the RBC quality during storage is to reduce oxidative stress by addition of antioxidants. Using this model system, we have recently shown a protective effect of trolox (manuscript in press) and to less extent quercetin [23] against oxidative damage of RBCs cold stored for up to 20 days. Although storage of RBCs, manually isolated from CPD-preserved whole blood, in plastic tubes does not represent a prevalent method utilized in blood banks, the usage of RBCs derived from one donor for screening different antioxidants in broad concentration ranges seems to be advantageous.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Pre-Storage Irradiation on the Oxidative Stress Markers, Membrane Integrity, Size and Shape of the Cold Stored Red Blood Cells

Antosik

Czubak

Gajek

et al. 2015

Transfus Med Hemother

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Background: To investigate the extent of oxidative damage and changes in morphology of manually isolated red blood cells (RBCs) from whole blood, cold stored (up to 20 days) in polystyrene tubes and subjected to pre-storage irradiation (50 Gy) and to compare the properties of SAGM-preserved RBCs stored under experimental conditions (polystyrene tubes) with RBCs from standard blood bag storage. Methods: The percentage of hemolysis as well as the extracellular activity of LDH, thiobarbituric acid-reactive substances, reduced glutathione (GSH), and total antioxidant capacity (TAC) were measured. Changes in the topology of RBC membrane, shape, and size were evaluated by flow cytometry and judged against microscopy images. Results: Irradiation caused significant LDH release as well as increased hemolysis and lipid peroxidation, GSH depletion, and reduction of TAC. Prolonged storage of irradiated RBCs resulted in phosphatidylserine exposure on the cell surface. By day 20, approximately 60% of RBCs displayed non-discoid shape. We did not notice significant differences in percentage of altered cells and cell volume between RBCs exposed to irradiation and those not exposed. Conclusion: Irradiation of RBC transfusion units with a dose of 50 Gy should be avoided. For research purposes such as studying the role of antioxidants, storage of small volumes of RBCs derived from the same donor would be more useful, cheaper, and blood-saving.

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“…These events were already seen and widely discussed in our earlier studies [14,23]. During storage, oxidative damage to lipids and proteins contributes to RBC injury.…”

Section: Discussionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Influence of Pre-Storage Irradiation on the Oxidative Stress Markers, Membrane Integrity, Size and Shape of the Cold Stored Red Blood Cells

Antosik

Czubak

Gajek

et al. 2015

Transfus Med Hemother

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“…16,17 Therefore, antioxidant strategies are still needed in order to prevent the deleterious effect of ionizing radiation. 18,19 Nanodiamonds (ND) are promising materials for biological applications due to their biocompatibility. 20 However, ND biocompatibility varies as a function of its cellular uptake, as well as its physicochemical properties, including size, shape and surface charge.…”

Section: Introductionmentioning

confidence: 99%

Carboxylated nanodiamonds inhibit γ-irradiation damage of human red blood cells

et al. 2016

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Nanodiamonds when carboxylated (cNDs) act as reducing agents and hence could limit oxidative damage in biological systems. Gamma (γ)-irradiation of whole blood or its components is required in immunocompetent patients to prevent transfusion-associated graft versus host disease (TA-GVHD). However, γ-irradiation of blood also deoxygenates red blood cells (RBCs) and induces oxidative damage, including abnormalities in cellular membranes and hemolysis. Using atomic force microscopy (AFM) and Raman spectroscopy, we examined the effect of cNDs on γ-irradiation mediated deoxygenation and morphological damage of RBCs. γ-Radiation induced several morphological phenotypes, including stomatocytes, codocytes and echinocytes. While stomatocytes and codocytes are reversibly damaged RBCs, echinocytes are irreversibly damaged. AFM images show significantly fewer echinocytes among cND-treated γ-irradiated RBCs. The Raman spectra of γ-irradiated RBCs had more oxygenated hemoglobin patterns when cND-treated, resembling those of normal, non-irradiated RBCs, compared to the non-cND-treated RBCs. cND inhibited hemoglobin deoxygenation and morphological damage, possibly by neutralizing the free radicals generated during γ-irradiation. Thus cNDs have the therapeutic potential to preserve the quality of stored blood following γ-irradiation.

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“…Previous efforts involving dehydroascorbate (DHA)/ascorbic acid (AA) [10] or GSH [11] had brought some encouraging results. Quite recent studies have shown the benefit of addition of AA [12,13], AA and N-acetylcysteine (NAC) [14] or quercetin [15] to the storage solutions but the effects such additives might have on the RBCs are not well understood. The ideal concentrations should be also established in which they shall be added to display the optimal effect.…”

Section: Introductionmentioning

confidence: 99%

Vitamin C and Trolox decrease oxidative stress and hemolysis in cold-stored human red blood cells

et al. 2017

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Objectives: To investigate the effects of sodium ascorbate (SA) (5-3125 μM) and a combination of SA and Trolox (25 and 125 μM) on oxidative changes generated in red blood cells (RBCs) followed by up to 20 days refrigerated storage. Methods: RBCs were isolated from CPD-preserved human blood. Percentage of hemolysis and extracellular activity of lactate dehydrogenase (LDH) were measured to assess the RBC membrane integrity. Lipid peroxidation (LPO), glutathione (GSH) and total antioxidant capacity (TAC) were quantified by thiobarbituric acid-reactive substances, Ellman's reagent and 2,2 ′ -azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS ·+ )-based assay, respectively. Results: SA failed to reduce the storage-induced hemolysis and RBC membrane permeability. Addition of SA resulted in a concentration-independent LPO inhibition and increased TAC. A combination of SA/ Trolox supplemented to the RBC medium significantly inhibited hemolysis, LDH leakage, LPO, GSH depletion and enhanced TAC. Discussion: The effects of vitamin C action are closely concentration-dependent and may be modulated by a variety of compounds (e.g. Hb degradation products) released from RBCs during the prolonged storage, changing its properties from anti-to pro-oxidative. The two different class antioxidants (SA/Trolox) could possibly cooperate to be good potential RBC storage additives ensuring both antiradical and membrane stabilizing protection.

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A moderate protective effect of quercetin against γ-irradiation- and storage-induced oxidative damage in red blood cells for transfusion

Cited by 11 publications

References 47 publications

Influence of Pre-Storage Irradiation on the Oxidative Stress Markers, Membrane Integrity, Size and Shape of the Cold Stored Red Blood Cells

Influence of Pre-Storage Irradiation on the Oxidative Stress Markers, Membrane Integrity, Size and Shape of the Cold Stored Red Blood Cells

Carboxylated nanodiamonds inhibit γ-irradiation damage of human red blood cells

Vitamin C and Trolox decrease oxidative stress and hemolysis in cold-stored human red blood cells

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