A Modification of the Mycoplasma Serum‐Drop Growth Inhibition Test

Møller, Birger R.

doi:10.1111/j.1365-2672.1979.tb01173.x

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Cited by 6 publications

(1 citation statement)

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“…mycoides SC (2) was used as a positive control, and monospecific rabbit serum against LppQ (1) was used as a negative control. Observation of growth inhibition was carried out under a light microscope as previously described (33,38).…”

Section: Methodsmentioning

confidence: 99%

A Metabolic Enzyme as a Primary Virulence Factor ofMycoplasma mycoidessubsp.mycoidesSmall Colony

Pilo¹,

Vilei²,

et al. 2005

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Section: Methodsmentioning

confidence: 99%

A Metabolic Enzyme as a Primary Virulence Factor ofMycoplasma mycoidessubsp.mycoidesSmall Colony

Pilo¹,

Vilei²,

et al. 2005

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Detection and speciation of common cell culture mycoplasmas by an enzyme-linked immunosorbent assay with biotin-avidin amplification and microporous membrane solid phase

Gabridge

Lundin

Gladd

1986

In Vitro Cell Dev Biol

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An enzyme-linked immunosorbent assay (ELISA) was developed in order to serve in detecting and speciating mycoplasmas isolated from cell cultures. Its main features included a biotin-streptavidin amplification step and a solid phase consisting of a microporous membrane. Cell samples in the form of suspensions were applied to nitrocellulose or ion exchange membranes immobilized in commercially-available microtiter, multiwell manifolds. The blocking buffer contained 1% purified alpha-casein. The primary antibodies were monoclonal and the polyclonal secondary antibody was biotinylated. The enzyme utilized was streptavidin-horseradish peroxidase. The substrate-dye complex consisted of either 4-chloro-1-naphthol and hydrogen peroxide or ortho phenylene diamine (OPD) and hydrogen peroxide. The presence of homologous antiserum in the reaction sequence gave clearly visible, colored reactions on the membrane when 50 ul with approximately 10(5) or more cfu/ml were present. This new biotin-avidin microporous membrane (BAMM-ELISA) test can be used both to detect mycoplasmas and to speciate them. The BAMM-ELISA is simple, rapid, sensitive, specific and economical. As such, it has potential for aiding in the control of mycoplasma contamination in cell culture, and could prove useful in clinical diagnostic applications as well.

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An overview of infectious agents of salpingitis, their biology, and recent advances in methods of detection

Mårdh

1980

American Journal of Obstetrics and Gynecology

123

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A Modification of the Mycoplasma Serum‐Drop Growth Inhibition Test

Cited by 6 publications

References 12 publications

A Metabolic Enzyme as a Primary Virulence Factor ofMycoplasma mycoidessubsp.mycoidesSmall Colony

A Metabolic Enzyme as a Primary Virulence Factor ofMycoplasma mycoidessubsp.mycoidesSmall Colony

Detection and speciation of common cell culture mycoplasmas by an enzyme-linked immunosorbent assay with biotin-avidin amplification and microporous membrane solid phase

An overview of infectious agents of salpingitis, their biology, and recent advances in methods of detection

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