A Modified Bollman's Technique for Cannulation of the Rat's Thoracic Duct: Lymph Flow Standardization

He, Gallo-Torres; On, Miller

doi:10.3181/00379727-130-33604

Cited by 18 publications

(5 citation statements)

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“…Since the same concentration of the vitamin E acetate ester was administered in each instance, it was possible to carry out appropriate comparisons under the several conditions studied. Infusion of saline at a constant rate has assured not only an adequate hydration, but a uniform lymph flow as well (18). The results of this study have provided definitive evidence that the presence of bile salts and pancreatic juice in the intestine is a requisite for the intestinal absorption and the lymphatic transport of vitamin E.…”

Section: Discussionmentioning

confidence: 58%

“…The detailed procedure for the cannulation of the abdominal thoracic duct in the rat has appeared (18). In some instances, rats with thoracic duct fistula but with reconstituted enterohepatic circulation were used.…”

Section: Surgerymentioning

confidence: 99%

“…In a last group of experiments, the effect on tocopherol absorption of infusion of bile alone into the duodenum was studied. For this purpose, bile, uncontaminated with pancreatic juice (18), was collected for up to one week from several rats and the pooled collections kept at 4 C until use. Three animals were cannulated in the thoracic duct and thrice cannulated in the common bile duct (Fig.…”

Section: The Effect Of the Infusion Of Pancreatic Juice Or Bile Alonementioning

confidence: 99%

“…It was, therefore, considered important to carry out a study on the influence of biliary or pancreatic secretions or both on the intestinal absorption of vitamin E. This investigation was facilitated by the recent development of a new technique for the cannulation of the abdominal thoracic duct inthe rat (18) and the availability of d,l,-a-tocopheryl-l',2"-aH-acetate.…”

Section: Introductionmentioning

confidence: 99%

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Obligatory role of bile for the intestinal absorption of vitamin E

Gallo-Torres

1970

Lipids

134

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Normal, white female rats subjected to cannulation of the abdominal thoracic duct have been utilized for a study on the essentiality of biliary and pancreatic secretions for the intestinal absorption of vitamin E. In all animals the thoracic duct lymph was collected. Some rats had the enterohepatic circulation undisturbed and in others bile or pancreatic juice or both were drained to the exterior by appropriate catheters in the common bile duct. On the first postoperative day, the animals received intragastrically an emulsion containing protein, carbohydrate, monoolein, 2 mg ofd,l‐α‐tocopheryl acetate plus 50 μC ofd,l‐α‐tocopheryl‐1’,2’‐3H‐acetate. The appearance of radioactive α‐tocopherol and its derivatives was determined in lymph, hourly, after emulsion administration. The obligatory role of bile in intestinal absorption ofd,l‐α‐tocopheryl‐1’,2’‐3H‐acetate has been established. Pancreatic juice seems to be necessary for the hydrolysis of the vitamin E acetate ester. The simultaneous infusion of bile and pancreatic juice promotes absorption of about 10% of the administered dose into the lymph. A chromatographic separation of the radioactive vitamin E fractions revealed that most of the vitamin E, which is actively transfered from the intestinal lumen to the lymph, is nonesterified. An oxidation product of α‐tocopherol, presumably itsp‐quinone, appears in small amounts in the lymph, but almost no labeled α‐tocopheryl acetate could be detected under these experimental conditions.

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Section: Discussionmentioning

confidence: 58%

Section: Surgerymentioning

confidence: 99%

Section: The Effect Of the Infusion Of Pancreatic Juice Or Bile Alonementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Obligatory role of bile for the intestinal absorption of vitamin E

Gallo-Torres

1970

Lipids

134

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“…a-Tocopherol (770 nmol) was dispersed in cream (triacylglycerol content 458 pnol) and was administered by stomach tube. Rats were prepared as described byGallo-Torres & Miller (1969). (B), Cholesterol; (a), phospholipids; (d), triacylglycerols.…”

mentioning

confidence: 99%