A modified broth dilution assay for antibiotic sensitivity testing of Mycobacterium avium-intracellulare using paraffin slide cultures

Ollar, Robert A.; Brown, Sheldon T.; Dale, Jeremy W.; Felder, Mitchell S.; Brown, I. N.; Edwards, F F; Armstrong, Donald

doi:10.1016/0041-3879(91)90008-g

Cited by 8 publications

(3 citation statements)

References 17 publications

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“…Isolates were tested by a modification of previously described broth microdilution methods (5,11). Isolates from frozen 7H9 broth stocks were plated as a confluent lawn on Middlebrook 7H10 agar plates.…”

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confidence: 99%

Polyclonal Mycobacterium avium infections in patients with AIDS: variations in antimicrobial susceptibilities of different strains of M. avium isolated from the same patient

Reyn¹,

Jacobs²,

Arbeit³

et al. 1995

J Clin Microbiol

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confidence: 99%

Polyclonal Mycobacterium avium infections in patients with AIDS: variations in antimicrobial susceptibilities of different strains of M. avium isolated from the same patient

Reyn¹,

Jacobs²,

Arbeit³

et al. 1995

J Clin Microbiol

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“…The final concentration of ethanol or dimethylformamide for the rifamycins was c0.5%. The dose and schedule of drug administration were selected to achieve therapeutic levels similar to those obtained in humans on the basis of the available pharmacokinetic data (6,15 The susceptibilities of SK095 to the study drugs, shown in Table 1, were determined by a previously described broth microdilution method (21).…”

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confidence: 99%

Azithromycin, rifabutin, and rifapentine for treatment and prophylaxis of Mycobacterium avium complex in rats treated with cyclosporine

Brown

Edwards

Bernard

et al. 1993

Antimicrob Agents Chemother

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Azithromycin, rifabutin, and rifapentine were used to treat or prevent disseminated Mycobacterium avium complex (MAC) infections produced in rats immunosuppressed with cyclosporine. Animals with bacteremic infections were treated 1 week after intravenous inoculation with 107 CFU of MAC with azithromycin, 100 mg/kg of body weight administered subcutaneously for 5 days and then 75 mg/kg on Monday, Wednesday, and Friday, or with rifabutin or rifapentine, 20 mg/kg administered intraperitoneally on Monday through Friday. All three drugs showed efficacy after 1 and 2 months. Rifabutin cleared the organisms from tissues more rapidly than azithromycin or rifapentine. To approximate prophylaxis, treatment was started 2 weeks before intravenous inoculation with 104 organisms. MAC infections were undetectable in treated animals after 4 months, while control animals had disseminated infections. These findings support the rationale for clinical trials of treatment and prophylaxis with these agents. The cyclosporine-treated rat appears to be a useful model in which to evaluate compounds for the treatment and prophylaxis of disseminated MAC infections.Disseminated infection with the Mycobacterium avium complex (MAC) is the most common cause of bacteremia in patients with AIDS, occurring in up to 50% of these patients (14). Survival of MAC-and human immunodeficiency virusinfected patients is half that of those with similar stages of human immunodeficiency virus infection but who do not have disseminated MAC infections (13). Treatment regimens with significant activity against MAC have been described, but relapses have occurred and none of the regimens have reliably eradicated the infection (9,10,11,20,25). Thus, there is an urgent need for more effective agents and approaches to the treatment and prevention of MAC infections.Successful treatment of established infections probably requires combination therapy with two or more effective drugs having different mechanisms of action (17). Rifabutin and rifapentine are new rifamycin analogs which demonstrate significant in vitro activity against MAC isolates.Studies of treatment with these agents in animal models have produced contradictory results (4,5,15,16). Azithromycin is a new azilide antibiotic which, despite its low in vitro activity against MAC, has shown promise as a treatment in animal studies (12) and early human trials (26). This efficacy may stem from its unusual pharmacokinetic profile, which can result in high concentrations in tissues (6).A new model of disseminated MAC infection has recently been described in Sprague-Dawley rats immunosuppressed with cyclosporine (CsA) (2). We evaluated the response to treatment with azithromycin, rifabutin, or rifapentine in CsA-treated rats with bacteremic MAC infections. As a model of prophylaxis, we also tested whether these drugs prevent the progression of low-grade infection.(Results of the studies described here were presented in MATERIALS AND METHODSDrugs. Azithromycin was provided by Pfizer Central Research, Groton, Conn. ...

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“…The affinity of Acinetobacter calcoaceticus T for the device is not unexpected as organisms of this genus often are isolated from hydrocarbon-contaminated soils. A more surprising result is the low affinity of Pseudomonas aeruginosa , Mycobacterium avium , and Mycobacterium intracel lulare to the device surface, as these bacteria have been successfully isolated using clinical paraffin baiting techniques ( , ). This result may be attributed to the variability of commercially available paraffins and the ability of the organisms in question to be attracted to hydrocarbons of certain chain lengths, perhaps not abundant on the biochip surface.…”

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confidence: 99%

Culture-Based MEMS Device to Track Gordonia in Activated Sludge

Polaczyk¹,

Kinkle²,

Papautsky³

et al. 2006

Environ. Sci. Technol.

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Previously, we reported on the use of microelectromechanical systems (MEMS) fabrication technologies to develop paraffin surfaces for miniaturization of culture-based detection and rapid quantification of Mycolata in environmental samples. In the current study, the novel culture-based biochip was calibrated with a broad range of pure cultures of Mycolata including Gordonia spp. isolated from activated sludge foam. The biochip successfully recovered Gordonia amarae spiked into a sample of mixed liquor collected from a municipal activated sludge system. Comparisons of these results with molecular biology-based assays including 16S rRNA-targeted fluorescence in situ hybridization (FISH) and antibody staining demonstrated that the biochip provides a more rapid and user-friendly platform for reliable identification and quantification of Mycolata in full-scale municipal activated sludge sewage treatment plants. The results of this work successfully demonstrate an alternative platform technology for inexpensive monitoring of environmental microorganisms using existing expertise by potential users in the area of bacterial cultivation.

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A modified broth dilution assay for antibiotic sensitivity testing of Mycobacterium avium-intracellulare using paraffin slide cultures

Cited by 8 publications

References 17 publications

Polyclonal Mycobacterium avium infections in patients with AIDS: variations in antimicrobial susceptibilities of different strains of M. avium isolated from the same patient

Polyclonal Mycobacterium avium infections in patients with AIDS: variations in antimicrobial susceptibilities of different strains of M. avium isolated from the same patient

Azithromycin, rifabutin, and rifapentine for treatment and prophylaxis of Mycobacterium avium complex in rats treated with cyclosporine

Culture-Based MEMS Device to Track Gordonia in Activated Sludge

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