A modified dose-response model that describes the relationship between haemagglutination inhibition titre and protection against influenza infection

Huang, Yin; Anderson, Steven A.; Forshee, Richard A.; Yang, Hong

doi:10.1111/jam.13628

Cited by 7 publications

(4 citation statements)

References 24 publications

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“…Among them Hemagglutination Inhibition (HI) assay is the most frequently applied to detect antibodies that inhibit the interaction of influenza HA with receptors on red blood cells or cultured cells. It is an indirect assay in which the highest dilution of serum that prevents hemagglutination is called the HI titer of the serum [ 19 , 20 , 22 ]. Another common serological assay to measure the total serum antibodies against specific influenza antigen is ELISA test [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice

et al. 2018

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BackgroundIn this work, we report an electrochemical biosensor for the detection of anti-hemagglutinin antibodies against the swine virus H1N1 present in mice sera immunized with mixture of His6-H1 HA in monomeric and oligomeric form. The oriented immobilization of the recombinant His-tagged hemagglutinin (His6-H1 HA) consists of: (i) formation of a mixed layer of 4-mercaptobutanol (MBT) and the thiol derivative of dipyrromethene (DPM); (ii) complexation of Cu (II) by DPM; (iii) immobilization of His6-H1 HA via coordination bonds between Cu (II) sites from DPM–Cu (II) complex and imidazole nitrogen atoms of a histidine tag; (iv) filling free spaces with bovine serum albumin. The interactions between recombinant His6- H1 HA covalently attached to the electrode surface and the anti-hemagglutinin H1 antibodies present in mice sera were explored with Osteryoung square-wave voltammetry.ResultsThis analytical device was able to detect the antibodies present in vaccinated mice sera diluted from 1 × 109 to 1 × 108 fold.ConclusionsThe unprecedented sensitivity of described biosensor is much better than widely use ELISA test and other analytical methods for determination of antibodies against the influenza A viruses. It has been proved that redox active DPM-Cu (II) monolayer is a universal platform suitable for stable and oriented immobilization of any His-tagged sensing elements. Thus, this universal layer could be a base of numerous analytical devices suitable for detection of antibodies against different viruses.

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Section: Introductionmentioning

confidence: 99%

Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice

et al. 2018

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“…The inconsistency of HI titre on predicting infection rate was also noted in the prior study (Huang et al . 2018).…”

Section: Resultsmentioning

confidence: 99%

“…Huang et al . (2018) presented a risk assessment of an adverse human response (infection/illness) when exposed to influenza virus as a function of pre‐exposure HI titre level by a modelling approach. A beta‐Poisson dose–response model (Equation ) was used in that study.

P false(d false) = 1 ‐ {(][, 1 + \frac{d}{N_{50}} (2^{1 / α} ‐ 1))}^{‐ α},

where P ( d ) is the probability of positive response (infection, illness, or death) and d is the dose; N 50 represents the dose needed to cause a 50% chance of the response; α is the slope parameter.…”

Section: Methodsmentioning

confidence: 99%

“…Huang et al . (2018) developed a predictive model that incorporates HI titre as a variable and a parameter representing the effect of HI titre on the host susceptibility and response into classical dose–response models. The current study investigates a statistical modelling approach to compare HI and PVN titres in a mouse challenge model using several influenza strains.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of hemagglutination inhibition and hemagglutinin pseudovirus neutralization titres in relation to protection against influenza in a mouse model

Huang

Lin

Wang

et al. 2021

Lett Appl Microbiol

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The hemagglutination inhibition (HI) test has long been used as a standard measure of antibody response for inactivated influenza vaccines. However, the HI test has limitations, such as insensitivity when using some H3N2 virus strains and failure to detect neutralizing antibodies that target regions distant from the receptor binding site. We therefore examined a hemagglutinin pseudovirus neutralization (PVN) test as a possible supplement or alternative to the HI test. We evaluated the association of HI or PVN titres with protection against influenza infection in mice based on morbidity (where the illness was defined as 25% body weight loss). We assessed this relationship using dose–response models incorporating HI or PVN titres as a variable. The morbidity was correlated with the pre‐exposure titres, and such a correlation was well described by a modified dose–response model. The mathematical modelling suggests that PVN titres consistently show a stronger association with in vivo protection as compared to HI titres in mice. Given our findings, the PVN test warrants further investigation as a tool for evaluating antibody responses to influenza vaccines containing hemagglutinin. The resulting models may also be useful for analyzing human clinical data to identify potentially protective antibody titres against influenza illness.

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Chitosan nanoparticle formulation attenuates poly (I:C) induced innate immune responses against inactivated virus vaccine in Atlantic salmon (Salmo salar)

Andresen

Gjøen

2021

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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A modified dose-response model that describes the relationship between haemagglutination inhibition titre and protection against influenza infection

Cited by 7 publications

References 24 publications

Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice

Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice

Comparison of hemagglutination inhibition and hemagglutinin pseudovirus neutralization titres in relation to protection against influenza in a mouse model

Chitosan nanoparticle formulation attenuates poly (I:C) induced innate immune responses against inactivated virus vaccine in Atlantic salmon (Salmo salar)

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