A modified fluorescein derivative with improved water-solubility for turn-on fluorescent determination of Hg 2+ in aqueous and living cells

Conversion of Isocyanide to Amine in The Presence of Water and Hg(II) Ions: Kinetics and Mechanism as Detected by Fluorescence Spectroscopy and Mass Spectrometry

“…For the description of the variation of the corresponding fluorescence intensity of 1,5-DIN with time, an irreversible, consecutive "ABCD" (A→B→C→D) reaction model was used Equations (8) and (9).…”

“…where k 1 , k 2 and k 3 are the rate coefficients for the reaction A→B, B→C and C→D, respectively. The values of k 1 , k 2 and k 3 were obtained by fitting Equations (8) and (9) to the experimental fluorescence intensity curves. For fitting of the parameters of Equations (6)- (9) to the experimental kinetic data and thus to determine the corresponding rate coefficients, a home-made parameter estimation software based on the Gauss-Newton-Marquardt procedure was applied [18].…”

“…Of these, in terms of selectivity and sensitivity, fluorometry appears to be the most cost effective and less time-consuming method. Various fluorometric probes have been designed and applied for Hg(II) determination in water such as those that are based on fluorescence quenching (turn-off) [7], enhancement (turn-on) [8] and recording the fluorescence emissions at two different wavelengths (ratiometric) [9][10][11][12]. The latter, ratiometric method is preferred since it provides self-calibration in a straightforward way and does not suffer from effects such as instrumental efficiencies and probe concentration.…”

Dual chemosensor for the rapid detection of mercury(ii) pollution and biothiols

“…In addition, mercury can affect human body organs through binding to the thiol groups of amino acids and proteins, thereby causing serious damage to the central nervous system, brain, kidney, and respiratory system. [14][15][16][17][18][19] The US Environmental Protection Agency (EPA) and the World Health Organization (WHO) have set the maximum allowed concentration of Hg 2+ in drinking water at 2 ppb (10 nM). 20 The determination of Hg 2+ pollution in environment is usually carried out by conventional advanced methods such as inductively coupled plasma mass spectroscopy (ICP-MS), 21 atomic absorption spectroscopy (AAS), 22 high performance liquid chromatography (HPLC), 23 and inductively coupled plasma-atomic emission spectrometry (ICP-AES).…”

“…25,26 Therefore, there is a critical need for sensitive cehmosensors for the rapid and selective detection of Hg 2+ pollution in the environment. 17,25,27 Cys is a biothiol that plays a crucial role in biological processes. Cys deficiency can result in many diseases such as liver cirrhosis, delayed growth and muscle loss.…”