A Modified Method for the Assay of Factor IX

Sen, Nicholas; Sen, Radharani; Denson, K. W. E.; Biggs, Rosemary

doi:10.1055/s-0038-1655032

Cited by 9 publications

(7 citation statements)

References 2 publications

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“…Two-stage assays of factor VIII and factor IX were performed according to Pool and Miller [20], and Sen et at. [27], respectively.…”

Section: Methodsmentioning

confidence: 97%

Changes in Coagulation Factors and Platelet Function in Response to Progestational Agents

McGrath¹,

Castaldi²

1975

Pathophysiol Haemos Thromb

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Levels of coagulation factors I, II, V, VII-X, VIII, IX, X, plasminogen and antithrombin III, were determined at midcycle in 17 test subjects using oral contraceptive agents, and in 10 control subjects. Immunoassay of factor II and antithrombin III was also carried out, and platelet aggregation quantitatively assessed. Significant increases were found in the activity of factors II, V, VII-X, X and plasminogen, but factor II antigen levels did not change. Platelet aggregation with collagen was significantly enhanced. No change occurred in antithrombin III activity or antigen levels.

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“…Two-stage assays of factor VIII and factor IX were performed according to Pool and Miller [20], and Sen et at. [27], respectively.…”

Section: Methodsmentioning

confidence: 97%

Changes in Coagulation Factors and Platelet Function in Response to Progestational Agents

McGrath¹,

Castaldi²

1975

Pathophysiol Haemos Thromb

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“…Factor I (fibrinogen) [16]; prothrombin time (PT), partial thromboplastin time (PTT), assays of factors II, V, Vili, XI, plasminogen [9]; factor VII [23]; factor IX [43]; factor X [22]; thrombin, recalcification test of Bidwell and Dike [6]; antithrombin III [13]; active factor IX [41]; protein [47]; phosphate, molybdate method of Varley [49]; calcium [52]; aluminium, determined spectrophotometrically by the British Aluminium Company Limited ; sterility tests, carried out by routine bacteriological techniques ; anti-A and anti-B, determined by saline haemagglutination; Au (1) antigen tests, carried out by the North London Blood Transfusion Centre, using the method of counter-current electrophoresis.…”

Section: Technical Methodsmentioning

confidence: 99%

Small Scale Preparation and Clinical Use of Factor IX-Prothrombin Complex

Barrowcliffe¹,

Stableforth²,

Dormandy³

1973

Vox Sanguinis

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A procedure is described for the preparation of a concentrate of factors II, VII, IX and X, using a modification of the method of Bruning and Loeliger, involving aluminium hydroxide adsorption of ACD plasma followed by phosphate elution and dialysis. The concentrate produced had an average factor IX activity of 7.4 U/ml with a yield of 61% and a purification of 34.5. Clinical trials in patients with either a congenital deficiency of factor IX or liver disease showed that the concentrate was well tolerated and was an effective haemostatic agent. The average factor IX response was 1.09% for each U/kg injected, giving a recovery of 48%.

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“…Das [16] and factor IX assay technique by Prof. N.N. Sen at Oxford [17]. First case of antithrombin III deficiency was reported from the country by Dr. Dipika Mohanty in PGIMER, Chandigarh [18].…”

Section: Coagulation Disordersmentioning

confidence: 99%

Haematology Research in India: Past, Present and Future

Ghosh

2011

Indian J Hematol Blood Transfus

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Haematology research in India is relatively recent in origin. However the pioneers in the field not only did exemplary work when compared to advanced western countries, they also made it a point to develop centres of excellence and human resources for future of haematology work in this country. In this brief overview an effort has been made to give a taste of quality and expanse of haematology research in this country. This review does not claim to have described every bit of haematology research in this country. Our pioneers worked under extremely difficult and trying circumstances on a subject which was limited to funding available from Indian Council of Medical Research. Now the times have changed, several funding agencies in the country are able to provide substantial fund for research. Modern state of the art basic research institutions are tying up with medical colleges for good quality research and the seeds which our pioneers had planted have grown into a mighty tree. It would not be an exaggeration to say we are on the threshold of the golden era of haematology research in this country.

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A Modified Method for the Assay of Factor IX

Abstract: SummaryA modification of the method for the assay of factor IX proposed by Biggs et al. (1961) (1) is described. The modification is likely to prove more reliable than the original method.

Cited by 9 publications

References 2 publications

Changes in Coagulation Factors and Platelet Function in Response to Progestational Agents

Changes in Coagulation Factors and Platelet Function in Response to Progestational Agents

Small Scale Preparation and Clinical Use of Factor IX-Prothrombin Complex

Haematology Research in India: Past, Present and Future

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