A modified microtiter-plate test for quantification of staphylococcal biofilm formation

Stepanović, Srdjan; Vuković, Dragana; Dakić, Ivana; Savić, Branislava; Švabić-Vlahović, Milena

doi:10.1016/s0167-7012(00)00122-6

Cited by 1,955 publications

(1,469 citation statements)

References 13 publications

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“…The light absorption was read by a spectrophotometer (SpectraMax plus 348 microplate reader, Molecular Devices) at 570 nm (ref. 58). The entire experiment was repeated independently.…”

Section: Mutant Phenotypingmentioning

confidence: 99%

Root-hair endophyte stacking in finger millet creates a physicochemical barrier to trap the fungal pathogen Fusarium graminearum

et al. 2016

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“…The light absorption was read by a spectrophotometer (SpectraMax plus 348 microplate reader, Molecular Devices) at 570 nm (ref. 58). The entire experiment was repeated independently.…”

Section: Mutant Phenotypingmentioning

confidence: 99%

Root-hair endophyte stacking in finger millet creates a physicochemical barrier to trap the fungal pathogen Fusarium graminearum

et al. 2016

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“…Biofilm production on polystyrene surface was determined using 96-well microtiter plates (Nunc), following the quantitative method described by Stepanovic et al (2000) with minor modifications. In short, 10 μL of an overnight culture at 37°C in Tryptic Soy Broth (TSB) supplemented with 0.25% glucose (for optimal growth of all species) was used to inoculate at 5% (cell concentration of ≈10 6 CFU mL −1 ) independent microtiter plate wells with 200 μL of the same medium.…”

Section: Detection and Quantification Of Biofilm Productionmentioning

confidence: 99%

Diversity and biofilm-forming capability of bacteria recovered from stainless steel pipes of a milk-processing dairy plant

Cherif-Antar

Moussa–Boudjemâa

Didouh

et al. 2015

Dairy Sci. & Technol.

105

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Bacteria may adhere to and develop biofilm structures onto dairy surfaces trying to protect themselves from adverse conditions such as pasteurization and CIP processes. Thus, biofilms are considered common sources of food contamination with undesirable bacteria. The purpose of this study was to evaluate the diversity of the microbiota attached to stainless steel surfaces in pre-and post-pasteurization pipe lines of a milk-processing plant. Seventy Gram-positive isolates were identified as Enterococcus faecalis (33), Bacillus cereus (26), Staphylococcus hominis (8), Staphylococcus saprophyticus (2), and Staphylococcus epidermidis-Staphylococcus aureus (1) species. Fifty-five Gram-negative isolates were identified to the species Escherichia coli (18), Klebsiella pneumoniae (13), Acinetobacter calcoaceticus (6), Serratia marcescens (6), Enterobacter spp. (5), Pseudomonas aeruginosa (4), Escherichia vulneris (2), and Proteus mirabilis (1). Fifty-five different strains were detected by the RAPD technique. These were subjected to an in vitro assay to evaluate their biofilm-forming capability. E. faecalis (7), A. calcoaceticus (4), K. pneumoniae (3), S. hominis (3), and P. aeruginosa (2) were the species in which more biofilm producer strains were encountered. The adhered microbiota was also assessed by the PCR-DGGE culture-independent technique. This analysis revealed a greater bacterial diversity than that revealed by culturing methods. In this way, in addition to the bacteria detected by culturing, DNA bands belonging to the genera Chrysobacterium and Streptomyces were also identified. This study emphasizes that knowledge of attached Dairy Sci. & Technol. (2016) 96:27-38 DOI 10.1007

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“…To complement our data on the effects of the test agents on biofilm formation, a mature biofilm assay was performed using S. mutans UA159 as previously described (Stepanovic et al 2000). Briefly, BHIS was inoculated with overnight cultures of S. mutans UA159 in a flat bottom 48-well cell culture plate and then incubated for 24 h at 37°C anaerobically without agitation to form mature biofilms.…”

Section: Mature Biofilm Assaysmentioning

confidence: 99%

Inhibition of Streptococcus mutans biofilm formation, extracellular polysaccharide production, and virulence by an oxazole derivative

Chen

Ren

Zhou

et al. 2015

Appl Microbiol Biotechnol

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Dental caries, a biofilm-related oral disease, is a result of disruption of the microbial ecological balance in the oral environment. Streptococcus mutans, which is one of the primary cariogenic bacteria, produces glucosyltransferases (Gtfs) that synthesize extracellular polysaccharides (EPSs). The EPSs, especially water-insoluble glucans, contribute to the formation of dental plaque, biofilm stability, and structural integrity, by allowing bacteria to adhere to tooth surfaces and supplying the bacteria with protection against noxious stimuli and other environmental attacks. The identification of novel alternatives that selectively inhibit cariogenic organisms without suppressing oral microbial residents is required. The goal of the current study is to investigate the influence of an oxazole derivative on S. mutans biofilm formation and the development of dental caries in rats, given that oxazole and its derivatives often exhibit extensive and pharmacologically important biological activities. Our data shows that one particular oxazole derivative, named 5H6, inhibited the formation of S. mutans biofilms and prevented synthesis of extracellular polysaccharides by antagonizing Gtfs in vitro, without affecting the growth of the bacteria. In addition, topical applications with the inhibitor resulted in diminished incidence and severity of both smooth and sulcal surface caries in vivo with a lower percentage of S. mutans in the animals' dental plaque compared to the control group (P<0.05). Our results showed that this oxazole derivative has the capacity to inhibit biofilm formation and cariogenicity of S. mutans.

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A modified microtiter-plate test for quantification of staphylococcal biofilm formation

Cited by 1,955 publications

References 13 publications

Root-hair endophyte stacking in finger millet creates a physicochemical barrier to trap the fungal pathogen Fusarium graminearum

Root-hair endophyte stacking in finger millet creates a physicochemical barrier to trap the fungal pathogen Fusarium graminearum

Diversity and biofilm-forming capability of bacteria recovered from stainless steel pipes of a milk-processing dairy plant

Inhibition of Streptococcus mutans biofilm formation, extracellular polysaccharide production, and virulence by an oxazole derivative

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