A Modified Miniscope System for Simultaneous Electrophysiology and Calcium Imaging in vivo

Wu, Xiaoting; Yang, Xiangyu; Song, Lulu; Wang, Yan; Li, Yamin; Liu, Yuanyuan; Yang, Xiaowei; Wang, Yijun; Li, Weidong

doi:10.3389/fnint.2021.682019

Cited by 28 publications

(15 citation statements)

References 24 publications

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“…Moreover, the E-Scope can be used with multiple different extracellular electrophysiological recording techniques including silicon microprobes as shown here, as well as tetrode arrays (Howe & Blair, 2022) or flexible electrode arrays (Chung et al, 2019). Several approaches now exist for linking different types of electrodes to GRIN lenses for simultaneous electrophysiological recordings and calcium imaging from the same brain region (Cobar et al, 2022;McCullough et al, 2022;Wu et al, 2021). The E-Scope would be able to couple with these electrode systems and imaging systems as well.…”

Section: Discussionmentioning

confidence: 99%

Correlated signatures of social behavior in cerebellum and anterior cingulate cortex

Hur

Safaryan

Yang

et al. 2023

Preprint

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The cerebellum has been implicated in the regulation of social behavior. Its influence is thought to arise from communication, via the thalamus, to forebrain regions integral in the expression of social interactions, including the anterior cingulate cortex (ACC). However, the signals encoded or the nature of the communication between the cerebellum and these brain regions remains poorly understood. Here, we describe an approach that overcomes technical challenges in exploring the coordination of distant brain regions at high temporal and spatial resolution during social behavior. We developed the E-Scope, an electrophysiology-integrated miniature microscope, to synchronously measure extracellular electrical activity in the cerebellum along with calcium imaging of the ACC. This single coaxial cable device combined these data streams to provide a powerful tool to monitor the activity of distant brain regions in freely behaving animals. During social behavior, we recorded the spike timing of multiple single units in cerebellar right Crus I (RCrus I) Purkinje cells (PCs) or dentate nucleus (DN) neurons while synchronously imaging calcium transients in contralateral ACC neurons. We find that during social interactions, a significant subpopulation of cerebellar PCs were robustly inhibited, while most modulated neurons in the DN were activated. As expected, we find that there are higher correlations in the activity of cerebellar and ACC neurons that are similarly excited or inhibited by social interaction than in the activity of those modulated in an opposing manner. Surprisingly, these distinctions in correlations largely disappear when only non-social bouts were analyzed, suggesting that cerebellar-cortical interactions were social behavior specific. Our work provides new insights into the complexity of cerebellar activation and co-modulation of the ACC during social behavior, and a valuable open-source tool for simultaneous, multimodal recordings in freely behaving mice.

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Section: Discussionmentioning

confidence: 99%

Correlated signatures of social behavior in cerebellum and anterior cingulate cortex

Hur

Safaryan

Yang

et al. 2023

Preprint

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“…Optical in vivo imaging using cranial and spinal windows can be compared to imaging with miniaturized microscopes (miniscopes). In general, using the cranial and spinal window provides more expandability to combine with other techniques and a larger optical FOV [ 128 , 129 ]. Furthermore, multimodal imaging is easier for simultaneous acquisition of various data (neuronal activity, cerebral hemodynamics, structural imaging).…”

Section: Discussionmentioning

confidence: 99%

Cranial and Spinal Window Preparation for in vivo Optical Neuroimaging in Rodents and Related Experimental Techniques

Yeon

Kim

et al. 2022

Exp Neurobiol

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Optical neuroimaging provides an effective neuroscience tool for multi-scale investigation of the neural structures and functions, ranging from molecular, cellular activities to the inter-regional connectivity assessment. Amongst experimental preparations, the implementation of an artificial window to the central nervous system (CNS) is primarily required for optical visualization of the CNS and associated brain activities through the opaque skin and bone. Either thinning down or removing portions of the skull or spine is necessary for unobstructed long-term in vivo observations, for which types of the cranial and spinal window and applied materials vary depending on the study objectives. As diversely useful, a window can be designed to accommodate other experimental methods such as electrophysiology or optogenetics. Moreover, auxiliary apparatuses would allow the recording in synchrony with behavior of large-scale brain connectivity signals across the CNS, such as olfactory bulb, cerebral cortex, cerebellum, and spinal cord. Such advancements in the cranial and spinal window have resulted in a paradigm shift in neuroscience, enabling in vivo investigation of the brain function and dysfunction at the microscopic, cellular level. This Review addresses the types and classifications of windows used in optical neuroimaging while describing how to perform in vivo studies using rodent models in combination with other experimental modalities during behavioral tests. The cranial and spinal window has enabled longitudinal examination of evolving neural mechanisms via in situ visualization of the brain. We expect transformable and multi-functional cranial and spinal windows to become commonplace in neuroscience laboratories, further facilitating advances in optical neuroimaging systems.

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“…Efforts have been made to combine optical and electrical recording modalities using transparent electrodes underlying a cranial window, but this limits the recording area to superficial structures 10 . The popular Miniscope 11 platform has also been used to achieve simultaneous optical and electrical recordings, 12 but this method is limited to one-photon imaging, and fabricating the device may prove difficult to labs without specialized microelectromechanical equipment for manufacturing the electrode array. More easily constructed single electrode-based designs have been developed, 13 but this method is restricted to head-fixed animals and faces limitations in isolating single units presented by single electrode recording modalities.…”

Section: Introductionmentioning

confidence: 99%

GRINtrode: a neural implant for simultaneous two-photon imaging and extracellular electrophysiology in freely moving animals

et al. 2022

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Significance: In vivo imaging and electrophysiology are powerful tools to explore neuronal function that each offer unique complementary information with advantages and limitations. Capturing both data types from the same neural population in the freely moving animal would allow researchers to take advantage of the capabilities of both modalities and further understand how they relate to each other.Aim: Here, we present a head-mounted neural implant suitable for in vivo two-photon imaging of neuronal activity with simultaneous extracellular electrical recording in head-fixed or fibercoupled freely moving animals.Approach: A gradient refractive index (GRIN) lens-based head-mounted neural implant with extracellular electrical recording provided by tetrodes on the periphery of the GRIN lens was chronically implanted. The design of the neural implant allows for recording from head-fixed animals, as well as freely moving animals by coupling the imaging system to a coherent imaging fiber bundle. Results:We demonstrate simultaneous two-photon imaging of GCaMP and extracellular electrophysiology of neural activity in awake head-fixed and freely moving mice. Using the collected information, we perform correlation analysis to reveal positive correlation between optical and local field potential recordings. Conclusion:Simultaneously recording neural activity using both optical and electrical methods provides complementary information from each modality. Designs that can provide such bimodal recording in freely moving animals allow for the investigation of neural activity underlying a broader range of behavioral paradigms.

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A Modified Miniscope System for Simultaneous Electrophysiology and Calcium Imaging in vivo

Cited by 28 publications

References 24 publications

Correlated signatures of social behavior in cerebellum and anterior cingulate cortex

Correlated signatures of social behavior in cerebellum and anterior cingulate cortex

Cranial and Spinal Window Preparation for in vivo Optical Neuroimaging in Rodents and Related Experimental Techniques

GRINtrode: a neural implant for simultaneous two-photon imaging and extracellular electrophysiology in freely moving animals

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