A Modified Nylon Bag Technique for Determining Apparent Digestibilities of Protein in Feedstuffs for Pigs

Sauer, W. C.; Jørgensen, Henry; Berzins, R.

doi:10.4141/cjas83-027

Cited by 182 publications

(120 citation statements)

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“…In the same study it was also found that incubation with a pancreatin solution gave similar results to those obtained with the mobile nylon-bag technique (Sauer et al 1983(Sauer et al , 1989, which can be considered as an intermediate between in vivo and in vitro methods. In fact, many in vitro methods are based on consecutive incubations with pepsin and pancreatin, suggesting that pancreatin contains all the necessary enzymes for solubilizing the potentially digestible nutrients.…”

Section: Enzyme Speclficitysupporting

confidence: 65%

Critical Evaluation of in Vitro Methods for Estimating Digestibility in Simple-Stomach Animals

1991

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Section: Enzyme Speclficitysupporting

confidence: 65%

Critical Evaluation of in Vitro Methods for Estimating Digestibility in Simple-Stomach Animals

1991

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“…During days 10-14, faecal and urine samples for the mass balance experiments were collected. During days 15 -16, the pigs were surgically fitted with a distal ileal cannula according to previously established procedures (14) . Following a post-surgical recovery period (days [17][18][19][20], the pigs resumed their previous feeding schedule for the duration of the experiment.…”

Section: Animals and Experimental Designmentioning

confidence: 99%

Nutrient utilisation and intestinal fermentation are differentially affected by the consumption of resistant starch varieties and conventional fibres in pigs

et al. 2008

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This study examined the influence of different resistant starch (RS) varieties and conventional fibres on the efficiency of nutrient utilisation and intestinal fermentation in pigs. Thirty-six pigs (30 kg) were fed poultry meal-based diets supplemented with 10 % granular resistant corn starch (GCS), granular resistant potato starch (GPS), retrograded resistant corn starch (RCS), guar gum (GG) or cellulose for 36 d according to a completely randomised block design. Distal ileal and total tract recoveries were similar (P. 0·05) among the RS varieties. Distal ileal starch recovery was higher (P,0·05) in pigs consuming the RS diets (27 -42 %) as compared with the control group (0·64 %). Consumption of GCS reduced (P, 0·05) apparent total tract digestibility and whole-body retention of crude protein in comparison with the control group. Consumption of GPS reduced (P,0·05) total tract Ca digestibility and whole-body retention of Ca and P compared with the control group. However, consumption of RCS increased (P,0·05) total tract Ca digestibility compared with the control group. Caecal butyrate concentration was increased (P, 0·05) following consumption of RCS and GG in comparison with the control group. Consumption of all the RS varieties reduced (P,0·05) caecal indole concentrations compared with the control. Caecal butyrate concentrations were positively correlated (P,0·05; r 0·63 -0·83) with thermal properties among the RS varieties. We conclude that nutrient utilisation and intestinal fermentation are differentially affected by the consumption of different RS varieties and types of fibres. Thermal properties associated with different RS varieties may be useful markers for developing RS varieties with specific functionality.Resistant starch and fibre: Nutrients: Butyrate and indole: Pigs Despite a wealth of research in both human subjects (1,2) and various animal model species (3,4) , the relevance of resistant starch (RS) to human health and disease prevention remains unclear. Much of this uncertainty likely stems from the exceptional diversity that is encountered among RS varieties. Although the heterogeneous nature of traditional dietary fibre supplements is well documented, it is equally important to recognise that RS varieties originating from different plant sources and manufactured with alternative processing technologies will possess unique physiochemical properties (5) . RS is generally classified into four distinct types: physically entrapped, inaccessible starch; native granular starch; retrograded starch; chemically modified starch (6) .Due to its physiochemical attributes, RS is a particularly useful functional food additive to manage weight loss (7) , improve insulin sensitivity (8) and reduce dyslipidaemia (9) . Furthermore, RS consumption is believed to improve intestinal health and function by increasing micronutrient absorption and favourably altering bacterial fermentation patterns in the large intestine (10,11) . However, as literature reports do not often stress the importance of starch variety ...

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“…The duodenal cannula was similar to the re-entrant pancreatic cannula descrided by Hee et al (1985). The surgical technique of Sauer et al (1983) was adapted for the insertion of the ileal cannula, whereas the duodenal cannula was inserted using the technique described by Hee et al (1985). The gilts were fasted for a 24 h period and then sedated one hour prior to surgery with a 1 ml intramuscular injection of Atravet (Ayerst Laboratories, Montreal, Quebec, Canada).…”

Section: Animals and Managementmentioning

confidence: 99%

“…The ileal cannula was designed according to Sauer et al (1983) as modified according to de Lange et al (1989). The duodenal cannula was similar to the re-entrant pancreatic cannula descrided by Hee et al (1985).…”

Section: Animals and Managementmentioning

confidence: 99%

Determination of bacterial amino acid contribution to ileal digesta from pigs using <sup>35</sup>S and DAPA marker techniques

Dugan¹,

Sauer²,

Dugan³

et al. 1994

J. Anim. Feed Sci.

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A study was conducted using two duodenal and ileal cannulated, 45 kg gilts, to determine the bacterial amino acid contribution to amino acids in ileal digesta by using 35 S and diaminopimelic acid (DAPA) as bacterial protein markers. Both the 35 S and DAPA marker techniques are based on the assumption that a representative purified bacterial pellet is collected from ileal digesta samples. The 35 S technique was modified from previous methods by isolating a specific methionine sulphone marker fraction. However, results from the 35 S-methionine sulphone marker technique exceeded sensible physiological estimates (mean of 190.6%) of the bacterial amino acid contribution to ileal digesta amino acids. Whereas, the mean bacterial amino acid contribution to ileal digesta was 29.4% when determined using the DAPA marker technique. The values determined by DAPA are within the 20 to 34% range of values that have been reported for pigs fed cereal based diets. The amino acid composition was different (P^0.05) between bacteria and undigested non-bacterial residues for isoleucine, aspartic acid, glycine, cysteine and tyrosine. The high concentration of glycine in non-bacterial residues relative to other amino acids measured and the corresponding low concentration in bacterial samples suggests that the bacterial pellet fraction was free of contamination. It was therefore concluded, that further research into determining the bacterial contribution to amino acids in ileal digesta is warranted.

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A Modified Nylon Bag Technique for Determining Apparent Digestibilities of Protein in Feedstuffs for Pigs

Cited by 182 publications

References 0 publications

Critical Evaluation of in Vitro Methods for Estimating Digestibility in Simple-Stomach Animals

Critical Evaluation of in Vitro Methods for Estimating Digestibility in Simple-Stomach Animals

Nutrient utilisation and intestinal fermentation are differentially affected by the consumption of resistant starch varieties and conventional fibres in pigs

Determination of bacterial amino acid contribution to ileal digesta from pigs using <sup>35</sup>S and DAPA marker techniques

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