A modified TOF-ratio to assess rocuronium-induced neuromuscular block: a comparison with the usual TOF-ratio

Schmartz, Denis; Bernard, Paul; Sghaier, Raouf; Fils, Jean‐François; Fuchs‐Buder, Thomas

doi:10.1016/j.accpm.2022.101088

Cited by 5 publications

(1 citation statement)

References 37 publications

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“…Two different monitors were simultaneously used to control and validate the neuromuscular blockade induced in macaques: TOF-Watch® (Alsevia pharma®, Paris, France), which measures T1 to T4 values and calculates the T4/T1 ratio, and ToFscan® (Idmed®, Marseille, France) that additionally calculates the T4/Tref ratio which is a reliable marker of neuromuscular blockade recovery and allows a comparison when the calibration could not be done or when the stimulation is too important 14 . Paediatric hardware was used, when available, to adapt to the macaque's morphology.…”

Section: Tof Monitoring Protocolmentioning

confidence: 99%

Optimization of neuromuscular blockade protocols in cynomolgus macaques: monitoring, doses and antagonism

Letscher,

Lemaitre,

Burban

et al. 2023

Preprint

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BackgroundNeuromuscular blocking agents (NMBAs) are a crucial component of anaesthesia and intensive care. NMBAs are a family of molecules defined by their ability to compete with acetylcholine for binding to the acetylcholine receptor at the neuromuscular junction. This functional homology relies on the presence of ammonium groups in all NMBAs that, however, display vastly different chemical structures. Among animal models, non-human primates (NHP) are an essential model for a great diversity of human disease models but remain poorly characterized for the effectiveness of the diverse NMBAs.MethodsSeven healthy male cynomolgus macaques were randomly assigned to this study. Experiments using macaques were approved by the local ethical committee (CEtEA #44). All animals were anaesthetized according to institutional guidelines, with ketamine and medetomidine, allowing IV line placement and tracheal intubation. Anaesthesia was maintained with isofluorane. Either rocuronium bromine or atracurium besylate was evaluated, with reversal with sugammadex. Monitoring was performed with two devices, TOF-Watch® and ToFscan®, measuring the T4/T1 and the T4/Tref ratios, respectively. Nonparametric Mann-Whitney statistical analyses were done when indicated.ResultsNMBA monitoring required adaptation compared to humans, such as stimulus intensity and electrodes placement, to be efficient and valid in Cynomolgus macaques. When administered, both NMBAs induced deep and persistent neuro-muscular blockade at equivalent doses to clinical doses in humans. Rocuronium-induced profound neuromuscular blockade could be reverted using the cyclodextrin sugammadex’s reversal agent. We report no adverse effects in these models by clinical observation, blood chemistry, or complete blood count.ConclusionThese results support the use of non-human primate models for neuromuscular blockade monitoring and testing novel NMBA or their reversal agents.

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Section: Tof Monitoring Protocolmentioning

confidence: 99%