A modified weight-of-evidence approach to evaluate the allergenic potential of food proteins

Sun, Ninghui; Tekutyeva, Liudmila; Wang, Shiping; Pu, Qiankun; Zhou, Chuanpeng; Wang, Jing; Che, Huilian

doi:10.1039/c4tx00211c

Cited by 9 publications

(3 citation statements)

References 43 publications

(42 reference statements)

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“…This indicated that TM induced mast cells to release a large number of cytokines and proteases, which significantly enhanced intestinal permeability and seriously destroyed the physiological function of mucosa. 36 However, the extent of histopathological changes in the spleen, lung, and jejunum of mice administered with TM and OVA by gavage was significantly lighter than those observed with intraperitoneal injection. In other words, it was suggested that gastrointestinal digestion may weaken the immunogenic potential of TM and reduce hypersensitivity in mice to some extent.…”

Section: ■ Results and Discussionmentioning

confidence: 88%

“…The villi of jejunum exhibited severe rupturing and exfoliation, with a large number of inflammatory cell infiltration and goblet cell proliferation. This indicated that TM induced mast cells to release a large number of cytokines and proteases, which significantly enhanced intestinal permeability and seriously destroyed the physiological function of mucosa . However, the extent of histopathological changes in the spleen, lung, and jejunum of mice administered with TM and OVA by gavage was significantly lighter than those observed with intraperitoneal injection.…”

Section: Resultsmentioning

confidence: 96%

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Digestion-Resistant Linear Epitopes as Dominant Contributors to Strong Allergenicity of Tropomyosin in Antarctic Krill (Euphausia superba)

Wang,

Lin,

Liu

et al. 2023

J. Agric. Food Chem.

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Although tropomyosin has been identified as a major allergen in Antarctic krill, the digestive fate of Antarctic krill tropomyosin and its relationship with allergenicity are unknown. In this study, Antarctic krill tropomyosin was administered to BALB/c mice via both gavage and intraperitoneal injection to explore its sensitizing and eliciting capacity, and its digestion products were analyzed for structural changes and digestion-resistant linear epitopes. Mice gavaged with tropomyosin exhibited lower levels of specific IgE and IgG1, mast cell degranulation, vascular permeability, and anaphylaxis symptoms than those in the intraperitoneal injection group. This may be due to the destruction of macromolecular aggregates, loose expansion of the tertiary structure, complete disappearance of α-helix, and significant changes in molecular force upon the digestion of tropomyosin. Nevertheless, the intragastric administration of Antarctic krill tropomyosin still triggered strong allergic reactions, which was attributed to the existence of seven digestion-resistant linear epitopes (Glu26–His44, Thr111–Arg125, Glu157–Glu164, Glu177–Gly186, Val209–Ile225, Arg244–Arg255, and Val261–Ile270).

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Section: ■ Results and Discussionmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 96%

Digestion-Resistant Linear Epitopes as Dominant Contributors to Strong Allergenicity of Tropomyosin in Antarctic Krill (Euphausia superba)

Wang,

Lin,

Liu

et al. 2023

J. Agric. Food Chem.

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“…And so far, the evaluation of the allergenicity of the BLG-free milk has not yet been investigated. For evaluating the allergenic potential of food proteins, animal models are a useful tool for a direct assessment of the sensitizing potential of food 22 , such as the Balb/c mice model 23 – 25 . One of the important characteristics responsible for the allergenicity of food proteins is their stability against pepsin digestion 26 .…”

Section: Introductionmentioning

confidence: 99%

Production of hypoallergenic milk from DNA-free beta-lactoglobulin (BLG) gene knockout cow using zinc-finger nucleases mRNA

Sun

Wang

Han

et al. 2018

Sci Rep

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The whey protein β-lactoglobulin (BLG) is a major milk allergen which is absent in human milk. Here, we for the first time generated DNA-free BLG bi-allelic knockout cow by zinc-finger nuclease (ZFNs) mRNA and produced BLG-free milk. According to the allergenicity evaluation of BLG-free milk, we found it can trigger lower allergic reaction of Balb/c mice including the rectal temperature drop and the allergen-specific immunoglobulin IgE production; BLG free-milk was easily digested by pepsin at 2 min, while BLG in control milk was still not completely digested after 60 min, and the binding of IgE from cow’s milk allergy (CMA) patients to BLG free-milk was significantly lower than that to the control milk. Meanwhile, the genome sequencing revealed that our animal is free of off-target events. Importantly, editing animal genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to foods produced by genome edited animals. Finally, the ZFNs-mediated targeting in cow could be transmitted through the germline by breeding. These findings will open up unlimited possibilities of modifying milk composition to make it more suitable for human health and also improve the functional properties of milk.

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Overviews of Food Allergy and Evaluating Methods Used in Allergenic Assessment of GMOs with Application Examples Conducted in Our Laboratory

Huang

2017

Safety Assessment of Genetically Modified Foods

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A modified weight-of-evidence approach to evaluate the allergenic potential of food proteins

Abstract: Schematic interpretation of a modified weight-of-evidence approach for evaluating the allergenic potential of food proteins.

Cited by 9 publications

References 43 publications

Digestion-Resistant Linear Epitopes as Dominant Contributors to Strong Allergenicity of Tropomyosin in Antarctic Krill (Euphausia superba)

Digestion-Resistant Linear Epitopes as Dominant Contributors to Strong Allergenicity of Tropomyosin in Antarctic Krill (Euphausia superba)

Production of hypoallergenic milk from DNA-free beta-lactoglobulin (BLG) gene knockout cow using zinc-finger nucleases mRNA

Overviews of Food Allergy and Evaluating Methods Used in Allergenic Assessment of GMOs with Application Examples Conducted in Our Laboratory

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